Anti-BPTF / FALZ 抗体 [2343C3a] (ab54356)

製品の概要

  • 製品名
    Anti-BPTF / FALZ antibody [2343C3a]
    BPTF / FALZ 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [2343C3a] to BPTF / FALZ
  • アプリケーション
    適用あり: WB, Dot blotmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant fragment (human) from an internal sequence.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at 4°C (stable for up to 12 months). Store at -20°C or -80°C.
  • バッファー
    Preservative: 0.05% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 精製度
    Protein G purified
  • 特記事項(精製)
    This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
  • ポリ/モノ
    モノクローナル
  • クローン名
    2343C3a
  • アイソタイプ
    IgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab54356 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent dilution. Predicted molecular weight: 311 kDa.
Dot blot Use at an assay dependent dilution.

ターゲット情報

  • 機能
    Histone-binding component of NURF (nucleosome-remodeling factor), a complex which catalyzes ATP-dependent nucleosome sliding and facilitates transcription of chromatin. Specifically recognizes H3 tails trimethylated on 'Lys-4' (H3K4me3), which mark transcription start sites of virtually all active genes. May also regulate transcription through direct binding to DNA or transcription factors.
  • 組織特異性
    Ubiquitously expressed, with highest levels in testis. Present in kidney, liver and brain. In the brain, highest levels are found in motor cortex (at protein level).
  • 配列類似性
    Belongs to the PBTF family.
    Contains 1 bromo domain.
    Contains 1 DDT domain.
    Contains 2 PHD-type zinc fingers.
  • 発生段階
    Abundantly expressed in the fetal brain. Present throughout the gray and white matter of the developing spinal cord at 18-22 gestational weeks. Expressed at low levels in adult brain and spinal cord and reexpressed in neurodegenerative diseases (at protein level).
  • ドメイン
    The second PHD-type zinc finger mediates binding to histone H3K4Me3. Has specificity for trimethyllysine; introducing a mutation in the Tyr-2876 residue can induce binding to dimethyllysine.
  • 翻訳後修飾
    Phosphorylation enhances DNA-binding.
    Highly susceptible to proteolysis.
  • 細胞内局在
    Cytoplasm. Nucleus. In brains of Alzheimer disease patients, present in a subset of amyloid-containing plaques.
  • Information by UniProt
  • 参照データベース
  • 別名
    • BPTF antibody
    • BPTF_HUMAN antibody
    • Bromodomain and PHD finger containing transcription factor antibody
    • Bromodomain and PHD finger-containing transcription factor antibody
    • Bromodomain PHD finger transcription factor antibody
    • FAC 1 antibody
    • fac1 antibody
    • FALZ antibody
    • Fetal Alz 50 clone 1 protein antibody
    • Fetal Alz 50 reactive clone 1 antibody
    • Fetal Alz-50 clone 1 protein antibody
    • Fetal Alzheimer antigen antibody
    • Nucleosome remodeling factor antibody
    • Nucleosome remodeling factor subunit BPTF antibody
    • Nucleosome remodeling factor, large subunit antibody
    • Nucleosome-remodeling factor subunit BPTF antibody
    • NURF 301 antibody
    • NURF301 antibody
    see all

Anti-BPTF / FALZ antibody [2343C3a] 画像

  • Anti-BPTF / FALZ antibody [2343C3a] (ab54356) + immunising recombinant protein fragment.

    Predicted band size : 311 kDa

プロトコール

Anti-BPTF / FALZ antibody [2343C3a] (ab54356) 使用論文

ab54356 has not yet been referenced specifically in any publications.

Product Wall

Application
Western blot
Sample
Human Cell lysate - whole cell (U2-OS cells)
Loading amount
50 µg
Specification
U2-OS cells
Gel Running Conditions
Non-reduced Denaturing (6)
Blocking step
Milk as blocking agent for 30 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: RT°C
Username

Ms. Ayelet Star

Verified customer

投稿 Feb 22 2013

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (U2-OS cells)
Specification
U2-OS cells
Fixative
Paraformaldehyde
Permeabilization
Yes - Triton 0.5%
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: RT°C
Username

Abcam user community

Verified customer

投稿 Feb 22 2013

Thank you very much for getting back to me and for confirming the sample type. It is much appreciated.
DISCOUNT CODE: 40403
Expiration date: 04/04/2013
Value: £227.00
I am very pleased to hear you would like to accept our offer and ...

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Thank you for your enquiry and your interest.
We can certainly consider offering a testing discount code. Would you be so kind to discuss with the customer what type human samples (cells, tissue sections, fixation) will be used for testing.
T...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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