Anti-Bovine Serum Albumin 抗体 [BSA-7G10] (ab3781)

製品の概要

  • 製品名Anti-Bovine Serum Albumin antibody [BSA-7G10]
    Bovine Serum Albumin 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [BSA-7G10] to Bovine Serum Albumin
  • アプリケーション適用あり: ELISA, WBmore details
  • 種交差性
    交差種: Mouse, Rat, Cow
    交差が予測される動物種: Rabbit, Human
  • 免疫原

    Full length native protein (purified) (Cow).

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーConstituents: 1.2114% Tris, 0.7507% Glycine, 2% Sucrose
  • Concentration information loading...
  • 精製度Protein A purified
  • ポリ/モノモノクローナル
  • クローン名BSA-7G10
  • ミエローマunknown
  • アイソタイプIgG1
  • 軽鎖の種類kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab3781 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/1000 - 1/10000.
WB 1/1200 - 1/2000.

ターゲット情報

  • 関連性Albumin is a soluble, monomeric protein which comprises about one-half of the blood serum protein. Albumin functions primarily as a carrier protein for steroids, fatty acids, and thyroid hormones and plays a role in stabilizing extracellular fluid volume. Albumin is synthesized in the liver as preproalbumin which has an N-terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles to produce the secreted albumin.
  • 細胞内局在Secreted
  • 参照データベース
  • 別名
    • ALB antibody
    • Albumin (32 AA) antibody
    • Albumin (AA 34) antibody
    • Albumin antibody
    • BSA antibody
    • Cell growth inhibiting protein 42 antibody
    • Growth inhibiting protein 20 antibody
    • PRO0883 antibody
    • PRO0903 antibody
    • PRO1341 antibody
    • Serum albumin antibody
    see all

Anti-Bovine Serum Albumin antibody [BSA-7G10] (ab3781) 使用論文

This product has been referenced in:
  • Walkup WG  et al. Phosphorylation of synaptic GTPase-activating protein (synGAP) by Ca2+/calmodulin-dependent protein kinase II (CaMKII) and cyclin-dependent kinase 5 (CDK5) alters the ratio of its GAP activity toward Ras and Rap GTPases. J Biol Chem 290:4908-27 (2015). WB . Read more (PubMed: 25533468) »
  • Wagner CS  et al. Intracellular regulation of cross-presentation during dendritic cell maturation. PLoS One 8:e76801 (2013). Read more (PubMed: 24098562) »

See all 3 Publications for this product

Product Wall

Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing
Sample Mouse Cell lysate - whole cell (MEF)
Specification MEF
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

投稿 Jul 15 2014

Application Western blot
Loading amount 10 µg
Gel Running Conditions Reduced Denaturing (12% resolving, 4.8% stacking gel)
Sample Rabbit Tissue lysate - whole (Heparinized rabbit blood)
Specification Heparinized rabbit blood
Treatment Rabbit blood digested by mosquitoes for 0 & 18hrs
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

投稿 Jul 02 2014

Thank you for your reply.

I heard back from the lab that ab3781 can be used with this standard protocol:
- Blocking for 20 min to 1 hour with TBST 1X with 5% dry milk
- Wash briefly with TBST 1X
- Incubation with the antibody a...

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Thank you for your inquiry.

I heard back from the lab that this antibody has been successfully tested on BSA in WB. The blocking buffer was TBS-Tween 20 with 5% dry milk. Is this what you had tried?

What dilutions of the antibody ha...

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Thank you for contacting us.

Please type Bovine Serum Album in the search box or click on the following link

http://www.abcam.com/index.html?pageconfig=searchresults

I hope this information is helpful to you. Please do n...

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Thank you for your phone call.
I am very sorry to hear that these antibodies did not work together in sandwich ELISA. I am sending you the following discount codes as compensation for trying the antibodies in an untested application. Unfortunately...

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Thank you for contacting us. These two anti-BSA antibodies are provided as ascites. Monoclonal antibodies can be produced by growing hybridoma cells within the peritoneal cavity of a mouse. When injected into a mouse, the hybridoma cells multiply an...

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Thank you for your enquiry. Unfortunately, epitope mapping was not done on this product, therefore we do not have any information on the exact epitope binding region. I am sorry I can't be of more assistance in this occasion but if there is anyt...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Purified protein (brain)
Loading amount 10 µg
Specification brain
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

投稿 Oct 16 2006

This antibody is only available in liquid form.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"