製品の概要

  • 製品名
    Anti-Bovine Papilloma Virus E2 antibody [1E4]
    Bovine Papilloma Virus E2 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [1E4] to Bovine Papilloma Virus E2
  • アプリケーション
    適用あり: WB, IP, ICC/IFmore details
  • 種交差性
    交差種: Cow
  • 免疫原

    Mapped to amino acids 250-280.

  • エピトープ
    Not Determined.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab980 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
IP
ICC/IF
  • 追加情報
    IF (ICC): 1/500- 1/1000 (1hr, RT). Dilute in a diluent containing 1% BSA in PBS.
    IP: Use at an assay dependent dilution to isolate E2Tag-tagged proteins and associated proteins/protein binding partners. Use protein G-agarose.
    WB: 1/1000 - 1/5000 (1hr, RT). Dilute in a diluent containing 2% non-fat dry milk in TBST.
    (Background can be reduced by adding NaCl up to a concentration of 1.5 M.)Incubate
    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 関連性
      The bovine papilloma virus E2 protein is the master regulator of papillomavirus replication and transcription. Binds to the E2RE response element (5'-ACCNNNNNNGGT-3') present in multiple copies in the regulatory region. Can either activate or repress transcription depending on E2RE's position with regards to proximal promoter elements. Repression occurs by sterically hindering the assembly of the transcription initiation complex. The E1-E2 complex binds to the origin of DNA replication.
    • 細胞内局在
      Host cell nucleus
    • 別名
      • BPVgp4 antibody
      • E2 antibody
      • Regulatory protein E2 antibody

    画像

    • All lanes : Anti-Bovine Papilloma Virus E2 antibody [1E4] (ab980)

      Lane 1 : Purified recombinant Bovine Papillomavirus E2 protein at 15000 µg
      Lane 2 : Purified recombinant Bovine Papillomavirus E2 protein at 10000 µg
      Lane 3 : Purified recombinant Bovine Papillomavirus E2 protein at 5000 µg
      Lane 4 : Purified recombinant Bovine Papillomavirus E2 protein at 1000 µg


      Predicted band size : 45 kDa

    参考文献

    This product has been referenced in:
    • Della Gatta G  et al. Reverse engineering of TLX oncogenic transcriptional networks identifies RUNX1 as tumor suppressor in T-ALL. Nat Med 18:436-40 (2012). ChIP . Read more (PubMed: 22366949) »
    • Kurg R  et al. Effect of bovine papillomavirus E2 protein-specific monoclonal antibodies on papillomavirus DNA replication. J Virol 73:4670-7 (1999). Read more (PubMed: 10233926) »

    See all 2 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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