製品の概要

  • 製品名Anti-BOULE antibody
    BOULE 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to BOULE
  • アプリケーション適用あり: ICCmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Cow, Dog
  • 免疫原

    Synthetic peptide:

    (C)QPEPIKTVWSIHY

    , corresponding to C terminal amino acids 271-283 of Human BOULE

  • ポジティブ・コントロール
    • IHC-P: FFPE human testis.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, 5mg/ml Tris, pH 7.3
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)This antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab28745 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC Use a concentration of 4 - 6 µg/ml.

ターゲット情報

  • 機能Probable RNA-binding protein, which may be required during spermatogenesis. May act by binding to the 3'-UTR of mRNAs and regulating their translation.
  • 組織特異性Testis specific. Not expressed in early embryos, primordial germ cells and spermatogonial cells. First expressed in the cytoplasm of spermatocytes and then persists through meiosis.
  • 配列類似性Belongs to the RRM DAZ family.
    Contains 1 DAZ-like domain.
    Contains 1 RRM (RNA recognition motif) domain.
  • 細胞内局在Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Bol (Drosophila boule homolog) like antibody
    • Bol antibody
    • Bol boule like antibody
    • Bol, boule like (Drosophila) antibody
    • bol, boule-like (Drosophila) antibody
    • Boll antibody
    • BOLL_HUMAN antibody
    • BOULE antibody
    • Boule like antibody
    • BOULE, Drosophila, homolog of antibody
    • Protein boule like antibody
    • Protein boule-like antibody
    • Putative uncharacterized protein BOLL antibody
    see all

Anti-BOULE antibody 画像

  • IHC image of BOULE staining in human testis formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab28745, 1µg/ml, for 15 mins at room temperature. A donkey anti-goat biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • In paraffin embedded Human Testis shows cytoplasm staining in spermatocytes, but not in spermatogonia. ab28745 (4µg/ml) staining following steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

Anti-BOULE antibody (ab28745) 使用論文

ab28745 has not yet been referenced specifically in any publications.

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