Anti-BORIS 抗体 [KT72] (ab111255)
Key features and details
- Rat monoclonal [KT72] to BORIS
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG2a
製品の概要
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製品名
Anti-BORIS antibody [KT72]
BORIS 一次抗体 製品一覧 -
製品の詳細
Rat monoclonal [KT72] to BORIS -
由来種
Rat -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Mouse BORIS
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading...
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精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
KT72 -
アイソタイプ
IgG2a -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab111255の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 0.1 - 1 µg/ml. Predicted molecular weight: 73 kDa.
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ICC/IF |
Use a concentration of 1 - 10 µg/ml.
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特記事項 |
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WB
Use a concentration of 0.1 - 1 µg/ml. Predicted molecular weight: 73 kDa. |
ICC/IF
Use a concentration of 1 - 10 µg/ml. |
ターゲット情報
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機能
Testis-specific DNA binding protein responsible for insulator function, nuclear architecture and transcriptional control, which probably acts by recruiting epigenetic chromatin modifiers. Plays a key role in gene imprinting in male germline, by participating in the establishment of differential methylation at the IGF2/H19 imprinted control region (ICR). Directly binds the unmethylated H19 ICR and recruits the PRMT7 methyltransferase, leading to methylate histone H4 'Arg-3' to form H4R3sme2. This probably leads to recruit de novo DNA methyltransferases at these sites (By similarity). Seems to act as tumor suppressor. In association with DNMT1 and DNMT3B, involved in activation of BAG1 gene expression by binding to its promoter. Required for dimethylation of H3 lysine 4 (H3K4me2) of MYC and BRCA1 promoters. -
組織特異性
Testis specific. Specifically expressed in primary spermatocytes. -
配列類似性
Belongs to the CTCF zinc-finger protein family.
Contains 11 C2H2-type zinc fingers. -
発生段階
Is up-regulated in primary spermatocytes (expression overlapping with the genome-wide erasure of methylation) to become silenced on activation of CTCF in post-meiotic germline cells (expression corresponding to DNA remethylation). -
細胞内局在
Cytoplasm. Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 140690 Human
- Omim: 607022 Human
- SwissProt: Q8NI51 Human
- Unigene: 131543 Human
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別名
- BORIS like protein antibody
- Brother of the regulator of imprinted sites antibody
- Cancer/testis antigen 27 antibody
see all
画像
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ICC/IF image of ab111255 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab111255, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96887, DyLight® 488 goat anti-rat IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% formaldehyde fixed (10 min) HeLa and HepG2 cells at 10µg/ml, and in 100% methanol fixed (5 min) Hek293 cells at 10µg/ml.
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All lanes : Anti-BORIS antibody [KT72] (ab111255)
Lane 1 : BORIS with N-terminal fused tag.
Lane 2 : BORIS with C-terminal fused tag.
Predicted band size: 73 kDa
Tag is a proprietary ~26 kDa tag.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab111255 は論文での使用が確認できていません。