Anti-Borealin/CDCA8 抗体 (ab74473)

製品の概要

  • 製品名
    Anti-Borealin/CDCA8 antibody
    Borealin/CDCA8 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Borealin/CDCA8
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide of 19 amino acids taken from near the N-terminus of Human Borealin/CDCA8. (NP_060571)

  • ポジティブ・コントロール
    • Rat kidney tissue lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab74473 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 31 kDa).

ターゲット情報

  • 機能
    Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. In the complex, it may be required to direct the CPC to centromeric DNA. Major effector of the TTK kinase in the control of attachment-error-correction and chromosome alignment.
  • 配列類似性
    Belongs to the borealin family.
  • 発生段階
    Cell-cycle regulated. Increases during G2/M phase and then reduces after exit from M phase.
  • ドメイン
    The C-terminal region (aa 207-280) represents the dimerization motif.
  • 翻訳後修飾
    Phosphorylated by TTK, essentially at Thr-88, Thr94, Thr-169 and Thr-230.
    Sumoylated by UBE2I and RANBP2. Desumoylated by SENP3 through the removal of SUMO2 and SUMO3.
  • 細胞内局在
    Nucleus > nucleolus. Cytoplasm. Cytoplasm > cytoskeleton > spindle. Chromosome > centromere. Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalizes with SENP3 in the nucleolus in interphase cells.
  • Information by UniProt
  • 参照データベース
  • 別名
    • BOR antibody
    • BOREA_HUMAN antibody
    • Borealin antibody
    • CDCA8 antibody
    • Cell division cycle associated 8 antibody
    • cell division cycle associated protein 8 antibody
    • Cell division cycle-associated protein 8 antibody
    • Dasra B antibody
    • Dasra-B antibody
    • DasraB antibody
    • FLJ10468 antibody
    • FLJ12042 antibody
    • hDasra B antibody
    • hDasra-B antibody
    • MESRGP antibody
    • OTTHUMP00000004514 antibody
    • pluripotent embryonic stem cell related gene 3 protein antibody
    • Pluripotent embryonic stem cell-related gene 3 protein antibody
    see all

画像

  • Lane 1 : Anti-Borealin/CDCA8 antibody (ab74473) at 1 µg/ml
    Lane 2 : Anti-Borealin/CDCA8 antibody (ab74473) at 2 µg/ml

    Lane 1 : Rat kidney lysate
    Lane 2 : Rat kidney lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 31 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)
  • Immunofluorescence of CDCA8 in Rat Kidney cells using ab74473 at 20 ug/ml.

プロトコール

参考文献

ab74473 has not yet been referenced specifically in any publications.

レビューと Q&A

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Purified protein (GST-Borealin of human origin)
Loading amount
1 µg
Specification
GST-Borealin of human origin
Gel Running Conditions
Reduced Denaturing (4-12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
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投稿 Apr 07 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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