製品の概要

  • 製品名Anti-Borealin/CDCA8 antibody
    Borealin/CDCA8 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Borealin/CDCA8
  • アプリケーション適用あり: WB, ELISA, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    A KLH conjugated synthetic peptide selected from the center region of human Borealin/CDCA8

  • ポジティブ・コントロール
    • HL60 cytoplasmic or nuclear lysates; Paraffin embedded Human lung carcinoma tissue

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab70910 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/100 - 1/500. Detects a band of approximately 31 kDa (predicted molecular weight: 31 kDa).
ELISA 1/1000.
IHC-P 1/10 - 1/50.

ターゲット情報

  • 機能Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. In the complex, it may be required to direct the CPC to centromeric DNA. Major effector of the TTK kinase in the control of attachment-error-correction and chromosome alignment.
  • 配列類似性Belongs to the borealin family.
  • 発生段階Cell-cycle regulated. Increases during G2/M phase and then reduces after exit from M phase.
  • ドメインThe C-terminal region (aa 207-280) represents the dimerization motif.
  • 翻訳後修飾Phosphorylated by TTK, essentially at Thr-88, Thr94, Thr-169 and Thr-230.
    Sumoylated by UBE2I and RANBP2. Desumoylated by SENP3 through the removal of SUMO2 and SUMO3.
  • 細胞内局在Nucleus > nucleolus. Cytoplasm. Cytoplasm > cytoskeleton > spindle. Chromosome > centromere. Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalizes with SENP3 in the nucleolus in interphase cells.
  • Information by UniProt
  • 参照データベース
  • 別名
    • BOR antibody
    • BOREA_HUMAN antibody
    • Borealin antibody
    • CDCA8 antibody
    • Cell division cycle associated 8 antibody
    • cell division cycle associated protein 8 antibody
    • Cell division cycle-associated protein 8 antibody
    • Dasra B antibody
    • Dasra-B antibody
    • DasraB antibody
    • FLJ10468 antibody
    • FLJ12042 antibody
    • hDasra B antibody
    • hDasra-B antibody
    • MESRGP antibody
    • OTTHUMP00000004514 antibody
    • pluripotent embryonic stem cell related gene 3 protein antibody
    • Pluripotent embryonic stem cell-related gene 3 protein antibody
    see all

Anti-Borealin/CDCA8 antibody 画像

  • Anti-Borealin/CDCA8 antibody (ab70910) at 1/100 dilution + HL60 cell line lysates at 35 µg

    Predicted band size : 31 kDa
    Observed band size : 31 kDa
    Additional bands at : 18 kDa,70 kDa. We are unsure as to the identity of these extra bands.
  • ab70910, at a 1/10 dilution, staining human Borealin/CDCA8 in lung carcinoma, using Immunohistochemistry, Formalin/PFA fixed Paraffin embedded tissue. ab70910 was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Anti-Borealin/CDCA8 antibody (ab70910) 使用論文

This product has been referenced in:

See 1 Publication for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Permeabilization Yes - 0.5% Triton in PBS
Specification HeLa
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative Paraformaldehyde
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投稿 Apr 06 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"