製品の概要

  • 製品名Anti-BOP1 antibody
    BOP1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to BOP1
  • アプリケーション適用あり: WB, IPmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Chimpanzee, Rhesus monkey, Gorilla
  • 免疫原

    Synthetic peptide corresponding to a region between residue 300 and 350 of Human BOP1 (NP_056016.1).

  • ポジティブ・コントロール
    • HeLa and 293T whole cell lysates.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab86653 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Predicted molecular weight: 84 kDa.
IP Use at 2-5 µg/mg of lysate.

ターゲット情報

  • 機能Component of the PeBoW complex, which is required for maturation of 28S and 5.8S ribosomal RNAs and formation of the 60S ribosome.
  • 配列類似性Belongs to the WD repeat BOP1/ERB1 family.
    Contains 7 WD repeats.
  • 細胞内局在Nucleus > nucleolus. Nucleus > nucleoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AU020183 antibody
    • AW146150 antibody
    • Block of proliferation 1 antibody
    • Block of proliferation 1 protein antibody
    • Bop1 antibody
    • Bop1 block of proliferation 1 antibody
    • BOP1_HUMAN antibody
    • D18861 antibody
    • Erb1p antibody
    • Kiaa0124 antibody
    • MGC109114 antibody
    • mKIAA0124 antibody
    • Ribosome biogenesis protein BOP1 antibody
    see all

Anti-BOP1 antibody 画像

  • All lanes : Anti-BOP1 antibody (ab86653) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg


    Predicted band size : 84 kDa
    Observed band size : 100,110 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 120 kDa,65 kDa,90 kDa. We are unsure as to the identity of these extra bands.
  • Western Blot detection of BOP1 in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab86653 at 3 µg/mg lysate for IP and at 0.4 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.
    Detection: Chemiluminescence with exposure time of 90 seconds

Anti-BOP1 antibody (ab86653) 使用論文

ab86653 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab86653.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"