製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to BOP1
  • アプリケーション
    適用あり: WB, IPmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Chimpanzee, Rhesus monkey, Gorilla
  • 免疫原

    Synthetic peptide corresponding to a region between residue 300 and 350 of Human BOP1 (NP_056016.1).

  • ポジティブ・コントロール
    • HeLa and 293T whole cell lysates.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab86653 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Predicted molecular weight: 84 kDa.
IP Use at 2-5 µg/mg of lysate.

ターゲット情報

  • 機能
    Component of the PeBoW complex, which is required for maturation of 28S and 5.8S ribosomal RNAs and formation of the 60S ribosome.
  • 配列類似性
    Belongs to the WD repeat BOP1/ERB1 family.
    Contains 7 WD repeats.
  • 細胞内局在
    Nucleus > nucleolus. Nucleus > nucleoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AU020183 antibody
    • AW146150 antibody
    • Block of proliferation 1 antibody
    • Block of proliferation 1 protein antibody
    • Bop1 antibody
    • Bop1 block of proliferation 1 antibody
    • BOP1_HUMAN antibody
    • D18861 antibody
    • Erb1p antibody
    • Kiaa0124 antibody
    • MGC109114 antibody
    • mKIAA0124 antibody
    • Ribosome biogenesis protein BOP1 antibody
    see all

画像

  • All lanes : Anti-BOP1 antibody (ab86653) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg


    Predicted band size : 84 kDa
    Observed band size : 100,110 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 120 kDa,65 kDa,90 kDa. We are unsure as to the identity of these extra bands.
  • Western Blot detection of BOP1 in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab86653 at 3 µg/mg lysate for IP and at 0.4 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.
    Detection: Chemiluminescence with exposure time of 90 seconds

参考文献

ab86653 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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