Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma (left) and mouse teratoma tissues labelling BOP1 with ab86652 at 1/200 (1µg/ml). Detection: DAB.
Immunoprecipitation - BOP1 antibody (ab86652)
Western Blot detection of BOP1 in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab86652 at 3 µg/mg lysate; Lane 2 represents control IgG IP. For the WB, ab86653 was used at 0.4 µg/ml.
Detection: Chemiluminescence with exposure time of 90 seconds.
All lanes : Anti-BOP1 antibody (ab86652) at 0.4 µg/ml
Lane 1 : immunoprecipitated BOP1 Lane 2 : control IgG