Anti-BOP1 抗体 (ab86652)
Key features and details
- Rabbit polyclonal to BOP1
- Suitable for: IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
-
製品名
Anti-BOP1 antibody
BOP1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to BOP1 -
由来種
Rabbit -
アプリケーション
適用あり: IP, IHC-Pmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Horse, Guinea pig, Cow, Pig, Rhesus monkey, Gorilla, Chinese hamster, Bat -
免疫原
Synthetic peptide corresponding to Human BOP1 aa 350-450.
Database link: Q14137 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
-
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab86652の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
IP |
Use at 2-5 µg/mg of lysate.
|
|
IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
特記事項 |
---|
IP
Use at 2-5 µg/mg of lysate. |
IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
-
機能
Component of the PeBoW complex, which is required for maturation of 28S and 5.8S ribosomal RNAs and formation of the 60S ribosome. -
配列類似性
Belongs to the WD repeat BOP1/ERB1 family.
Contains 7 WD repeats. -
細胞内局在
Nucleus > nucleolus. Nucleus > nucleoplasm. - Information by UniProt
-
参照データベース
- Entrez Gene: 23246 Human
- Entrez Gene: 12181 Mouse
- Entrez Gene: 300050 Rat
- Omim: 610596 Human
- SwissProt: Q14137 Human
- SwissProt: P97452 Mouse
- SwissProt: Q562C2 Rat
- Unigene: 645279 Human
see all -
別名
- AU020183 antibody
- AW146150 antibody
- Block of proliferation 1 antibody
see all
画像
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma (left) and mouse teratoma tissues labelling BOP1 with ab86652 at 1/200 (1µg/ml). Detection: DAB.
-
Western Blot detection of BOP1 in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab86652 at 3 µg/mg lysate; Lane 2 represents control IgG IP. For the WB, ab86653 was used at 0.4 µg/ml.
Detection: Chemiluminescence with exposure time of 90 seconds.
All lanes : Anti-BOP1 antibody (ab86652) at 0.4 µg/ml
Lane 1 : immunoprecipitated BOP1
Lane 2 : control IgG
Observed band size: 101 kDa why is the actual band size different from the predicted?
Additional bands at: 200 kDa, 55 kDa, 60-71 kDa. We are unsure as to the identity of these extra bands.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (2)
ab86652 は 2 報の論文で使用されています。
- Chen B et al. The Long Noncoding RNA CCAT2 Induces Chromosomal Instability Through BOP1-AURKB Signaling. Gastroenterology 159:2146-2162.e33 (2020). PubMed: 32805281
- Corsini NS et al. Coordinated Control of mRNA and rRNA Processing Controls Embryonic Stem Cell Pluripotency and Differentiation. Cell Stem Cell 22:543-558.e12 (2018). PubMed: 29625069