製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Bok
  • アプリケーション
    適用あり: ELISA, IHC-P, WBmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide from the N-terminal region (BH3 domain) of human Bok.

  • ポジティブ・コントロール
    • HL-60 cell lysate; Parraffin embedded human hepatocarcinoma tissue.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 精製度
    Protein G purified
  • 特記事項(精製)
    ab71646 is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab71646 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/1000.
IHC-P 1/50 - 1/100.
WB 1/100 - 1/500. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).

ターゲット情報

画像

  • Anti-Bok antibody (ab71646) at 1/100 dilution + HL-60 cell lysate at 12.5 µg

    Secondary
    HRP-anti-rabbit

    Predicted band size : 23 kDa
    Observed band size : 23 kDa
  • ab71646 at 1/50 dilution, staining Bok in human hepatocarcinoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue, followed by peroxidase-conjugated secondary antibody and DAB staining.

参考文献

ab71646 has not yet been referenced specifically in any publications.

レビューと Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Hepatocytes and Bone marrow cells)
Loading amount
50 µg
Specification
Hepatocytes and Bone marrow cells
Gel Running Conditions
Reduced Denaturing (4-20% Tris Glycine Gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 May 05 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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