The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 121 kDa.
Use at an assay dependent dilution.
Use at an assay dependent concentration.
Brother of CDO (BOC) interacts with Hedgehog (Hh) via a specific FNIII domain and belongs to the Ihog (interference hedgehog) family of proteins. Zebrafish BOC is expressed predominantly in the developing neural plate and later in the neural tube and developing brain.
Cell membrane; Single pass type I membrane protein. Note: Enriched at sites of cell-cell contact.
Anti-BOC antibody (ab77556) at 0.3 µg/ml + human ileum lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 121 kDa Observed band size: 121 kDa
Exposure time: 1 hour
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BOC antibody (ab77556)This image is courtesy of an Abreview submitted by Thomas Quentin
ab77556 staining BOC in Human smotth muscle cells by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 100% antibody diluent for 30 minutes at room temperature; antigen retrieval was with Proteinase K + Citrate Buffer pH 6. Samples were incubated with primary antibody (1/200) for 12 hours at 4°C. An undiluted HRP-conjugated Mouse anti-goat monocloanl was used as the secondary antibody.