製品の概要

  • 製品名Anti-BOB1 antibody [EP3300]
    BOB1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP3300] to BOB1
  • アプリケーション適用あり: WB, IHC-P, ICCmore details
    適用なし: Flow Cyt or IP
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide corresponding to residues in human BOB1.

  • ポジティブ・コントロール
    • Ramos cell lysate. Human tonsil tissue.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab76010 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
IHC-P
ICC
  • 追加情報ICC: 1/100 - 1/250.
    IHC-P: 1/100 - 1/250. Perform heat mediated antigen retrieval using 0.01M Sodium Citrate Buffer, pH 6.0 before commencing with IHC staining protocol.
    WB: 1/5000 - 1/30000. Predicted molecular weight: 28 kDa.

    Is unsuitable for Flow Cyt or IP.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Transcriptional coactivator that specifically associates with either OCT1 or OCT2. It boosts the OCT1 mediated promoter activity and to a lesser extent, that of OCT2. It has no intrinsic DNA-binding activity. It recognizes the POU domains of OCT1 and OCT2. It is essential for the response of B-cells to antigens and required for the formation of germinal centers.
    • 組織特異性B-cell specific.
    • 関連疾患Note=A chromosomal aberration involving POU2AF1/OBF1 may be a cause of a form of B-cell leukemia. Translocation t(3;11)(q27;q23) with BCL6.
    • 配列類似性Belongs to the POU2AF1 family.
    • 翻訳後修飾Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation.
    • 細胞内局在Nucleus.
    • Information by UniProt
    • 参照データベース
    • 別名
      • B cell Oct binding protein 1 antibody
      • B cell specific coactivator OBF 1 antibody
      • B cell specific coactivator OBF1 antibody
      • B-cell-specific coactivator OBF-1 antibody
      • BOB 1 antibody
      • BOB-1 antibody
      • OBF 1 antibody
      • OBF1 antibody
      • OBF1_HUMAN antibody
      • OCA B antibody
      • OCA-B antibody
      • OCAB antibody
      • OCT binding factor 1 antibody
      • OCT-binding factor 1 antibody
      • POU class 2 associating factor 1 antibody
      • POU domain class 2 associating factor 1 antibody
      • POU domain class 2-associating factor 1 antibody
      • Pou2af1 antibody
      see all

    Anti-BOB1 antibody [EP3300] 画像

    • Anti-BOB1 antibody [EP3300] (ab76010) at 1/30000 dilution + Ramos cell lysate at 10 µg

      Secondary
      Goat anti-rabbit HRP at 1/1000 dilution

      Predicted band size : 28 kDa
      Observed band size : 35 kDa (why is the actual band size different from the predicted?)
    • ab76010, at 1/100 dilution, staining BOB1 in human tonsil by immunohistochemistry using paraffin-embedded tissue.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    Anti-BOB1 antibody [EP3300] (ab76010) 使用論文

    ab76010 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab76010.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"