製品の概要
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製品名Anti-BNP antibody [EPR3735]
BNP 一次抗体 製品一覧 -
製品の詳細Rabbit monoclonal [EPR3735] to BNP
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由来種Rabbit
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アプリケーション適用あり: WBmore details
適用なし: IHC-P or IP -
種交差性交差種: Human
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免疫原
Synthetic peptide within Human BNP aa 50-150. The exact sequence is proprietary.
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ポジティブ･コントロール
- Human plasma and fetal heart lysate.
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特記事項
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at 4°C prior to reconstitution. Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
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バッファーPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
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精製度Tissue culture supernatant
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ポリ/モノモノクローナル
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クローン名EPR3735
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アイソタイプIgG
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研究分野
アプリケーション
Our Abpromise guarantee covers the use of ab92500 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Is unsuitable for IHC-P or IP.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
ターゲット情報
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関連性Brain natriuretic peptide (BNP) circulates in blood as a peptide hormone with natriuretic, vasodilatory and renin inhibitory properties. BNP is secreted predominantly by the left ventricular myocytes in response to volume expansion and pressure overload. BNP belongs to a family of structurally similar peptide hormones, which includes atrial natriuretic peptide (ANP), BNP, C type natriuretic peptide (CNP) and urodilatin. These peptides are characterized by a common 17 amino acid ring structure with a disulfide bond between two cystein residues. This ring structure shows high homology between different natriuretic peptides (eleven of the 17 amino acid residues are homologous in the ring of each of the natriuretic peptides). BNP is a 32 amino acid peptide with disulfide bond between the cysteine residues Cys10 and Cys26. In earlier studies it has been demonstrated that BNP concentration in blood increases with the severity of congestive heart failure. Quantitative measurement of BNP in blood provides an objective indicator of congestive heart failure severity.
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細胞内局在Secreted
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参照データベース
- Entrez Gene: 4879 Human
- Omim: 600295 Human
- SwissProt: P16860 Human
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別名
- BNP(1-28) antibody
- BNP(1-29) antibody
- BNP(1-30) antibody
see all
画像
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![Western blot - Anti-BNP antibody [EPR3735] (ab92500)](http://a.abcam.com/ps/products/92/ab92500/Images/BNP-Primary-antibodies-ab92500-1.jpg)
Western blot - Anti-BNP antibody [EPR3735] (ab92500)All lanes : Anti-BNP antibody [EPR3735] (ab92500) at 1/2000 dilution
Lane 1 : Human plasma
Lane 2 : Human fetal
heart lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 15 kDa
Observed band size: 30 kDa (why is the actual band size different from the predicted?)
プロトコール
参考文献
ab92500 has not yet been referenced specifically in any publications.
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"