製品の概要

  • 製品名Anti-BNP antibody
    BNP 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to BNP
  • アプリケーション適用あり: ELISA, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant full length protein (Human) with tag.

  • ポジティブ・コントロール
    • BNP transfected 293T cell lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab66240 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA
WB
  • 追加情報ELISA: Use at an assay dependent dilution. Sandwich ELISA: The detection limit for recombinant tagged BNP is approximately 3ng/ml as a capture antibody.
    WB: Use at a concentration of 1 - 5 µg/ml. Detects a band of approximately 15 kDa (predicted molecular weight: 15 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 関連性Brain natriuretic peptide (BNP) circulates in blood as a peptide hormone with natriuretic, vasodilatory and renin inhibitory properties. BNP is secreted predominantly by the left ventricular myocytes in response to volume expansion and pressure overload. BNP belongs to a family of structurally similar peptide hormones, which includes atrial natriuretic peptide (ANP), BNP, C type natriuretic peptide (CNP) and urodilatin. These peptides are characterized by a common 17 amino acid ring structure with a disulfide bond between two cystein residues. This ring structure shows high homology between different natriuretic peptides (eleven of the 17 amino acid residues are homologous in the ring of each of the natriuretic peptides). BNP is a 32 amino acid peptide with disulfide bond between the cysteine residues Cys10 and Cys26. In earlier studies it has been demonstrated that BNP concentration in blood increases with the severity of congestive heart failure. Quantitative measurement of BNP in blood provides an objective indicator of congestive heart failure severity.
    • 細胞内局在Secreted
    • 参照データベース
    • 別名
      • BNP(1-28) antibody
      • BNP(1-29) antibody
      • BNP(1-30) antibody
      • BNP(1-32) antibody
      • BNP(2-31) antibody
      • BNP(3-29) antibody
      • BNP(3-30) antibody
      • BNP(3-32) antibody
      • BNP(4-27) antibody
      • BNP(4-29) antibody
      • BNP(4-30) antibody
      • BNP(4-31) antibody
      • BNP(4-32) antibody
      • BNP(5-29) antibody
      • BNP(5-31) antibody
      • BNP(5-32) antibody
      • Brain natriuretic peptide 32 antibody
      • Brain type natriuretic peptide antibody
      • Gamma brain natriuretic peptide antibody
      • Natriuretic peptide B antibody
      • Natriuretic peptide precursor B antibody
      • natriuretic peptide, brain type antibody
      • Natriuretic peptides B antibody
      • natriuretic protein antibody
      • NPPB antibody
      see all

    Anti-BNP antibody 画像

    • All lanes : Anti-BNP antibody (ab66240) at 1 µg/ml

      Lane 1 : BNP transfected 293T cell lysate
      Lane 2 : Non-transfected 293T cell lysate

      Lysates/proteins at 50 µg per lane.

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

      Predicted band size : 15 kDa
      Observed band size : 15 kDa
      Additional bands at : 10 kDa. We are unsure as to the identity of these extra bands.

    Anti-BNP antibody (ab66240) 使用論文

    ab66240 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab66240.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"