製品の概要

  • 製品名Anti-BNIP3 antibody [EPR4034]
    BNIP3 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR4034] to BNIP3
  • アプリケーション適用あり: WB, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human BNIP3 aa 1-100.

  • ポジティブ・コントロール
    • WB: Jurkat cells, Jurkat cells treated with etoposide, SH-SY5Y cells, SH-SY5Y cells treated with camptothecin and MCF-7 cells treated with Cocl2 lysates and rat kidney, mouse spleen and mouse kidney tissue lysates. IHC-P: Human renal adenocarcinoma and kidney tissues. ICC/IF: HeLa and SH-SY5Y cells.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab109362 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Detects a band of approximately 30 kDa (predicted molecular weight: 22 kDa).

For unpurified use at 1/1000 - 1/10000.

IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See protocols (link: http://www.abcam.com/protocols/ihc-antigen-retrieval-protocol).

(Heat to 98°C, allow to cool for 10-20 minutes).

For unpurified use at 1/50 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF 1/150.

For unpurified use at 1/100 - 1/250.

ターゲット情報

  • 機能Apoptosis-inducing protein that, which can overcome BCL2 suppression. May play a role in repartitioning calcium between the two major intracellular calcium stores in association with BCL2.
  • 配列類似性Belongs to the NIP3 family.
  • 細胞内局在Mitochondrion. Mitochondrion membrane. Coexpression with the EIB 19-kDa protein results in a shift in NIP3 localization pattern to the nuclear envelope. Colocalizes with ACAA2 in the mitochondria.
  • Information by UniProt
  • 参照データベース
  • 別名
    • BCL2 Adenovirus E1B 19kDa Interacting Protein 3 antibody
    • BCL2/adenovirus E1B 19 kDa protein interacting protein 3 antibody
    • BCL2/adenovirus E1B 19 kDa protein-interacting protein 3 antibody
    • BNIP 3 antibody
    • BNIP3 antibody
    • BNIP3_HUMAN antibody
    • NIP 3 antibody
    • NIP3 antibody
    see all

Anti-BNIP3 antibody [EPR4034] 画像

  • Anti-BNIP3 antibody [EPR4034] (ab109362) at 1/1000 dilution (purified) + SH-SY5Y cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size : 22 kDa
    Observed band size : 30 kDa (why is the actual band size different from the predicted?)

    Blocking and diluting buffer: 5% NFDM/TBST.

  • Anti-BNIP3 antibody [EPR4034] (ab109362) at 1/2000 dilution (purified) + Jurkat cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size : 22 kDa
    Observed band size : 30 kDa (why is the actual band size different from the predicted?)

    Blocking and diluting buffer: 5% NFDM/TBST.

  • All lanes : Anti-BNIP3 antibody [EPR4034] (ab109362) at 1/2000 dilution (purified)

    Lane 1 : Rat kidney tissue lysate
    Lane 2 : Mouse kidney tissue lysate
    Lane 3 : Mouse spleen tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size : 22 kDa
    Observed band size : 30 kDa (why is the actual band size different from the predicted?)

    Blocking and diluting buffer: 5% NFDM/TBST.

  • All lanes : Anti-BNIP3 antibody [EPR4034] (ab109362) at 1/1000 dilution (unpurified)

    Lane 1 : Jurkat cell lysate treated with etoposide
    Lane 2 : Jurkat cell lysate
    Lane 3 : SH-SY5Y cell lysate treated with camptothecin
    Lane 4 : SH-SY5Y cell lysate
    Lane 5 : MCF-7 cell lysate treated with Cocl2

    Lysates/proteins at 10 µg per lane.


    Predicted band size : 22 kDa
    Observed band size : 30 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human renal adenocarcinoma tissue labelling BNIP3 with purified ab109362 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling BNIP3 with unpurified ab109362 at a 1/50 dilution.

  • Immunocytochemistry/Immunofluorescence analysis of SH-SY5Y cells labelling BNIP3 with unpurified ab109362 at a 1/100 dilution.

Anti-BNIP3 antibody [EPR4034] (ab109362) 使用論文

This product has been referenced in:
  • Chen SJ  et al. A splice variant of the human ion channel TRPM2 modulates neuroblastoma tumor growth through hypoxia-inducible factor (HIF)-1/2a. J Biol Chem 289:36284-302 (2014). WB . Read more (PubMed: 25391657) »
  • Huang J  et al. Effect of a low-protein diet supplemented with ketoacids on skeletal muscle atrophy and autophagy in rats with type 2 diabetic nephropathy. PLoS One 8:e81464 (2013). WB ; Rat . Read more (PubMed: 24303049) »

See all 3 Publications for this product

Product Wall

Thank you for contacting us. The immunogen sequence is between amino acids 30 - 55 of human BNIP3 (UniProt: Q12983). I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"