Rabbit polyclonal to BNIP3 - Carboxyterminal end
Mouse, Rat, Human
corresponding to a sequence near the C-terminal of human BNIP3, identical to the related mouse and rat sequence.
Mammary cancer tissue, rat thymus tissue lysate.
Lyophilised:0.2ml of distilled water will yield a concentration of 500µg/ml.
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 30, 60 kDa (predicted molecular weight: 22 kDa).
Use a concentration of 1 µg/ml.
Use at an assay dependent concentration. PubMed: 23691217
Apoptosis-inducing protein that, which can overcome BCL2 suppression. May play a role in repartitioning calcium between the two major intracellular calcium stores in association with BCL2.
Belongs to the NIP3 family.
Mitochondrion. Mitochondrion membrane. Coexpression with the EIB 19-kDa protein results in a shift in NIP3 localization pattern to the nuclear envelope. Colocalizes with ACAA2 in the mitochondria.
Information by UniProt
BCL2 Adenovirus E1B 19kDa Interacting Protein 3 antibody
BCL2/adenovirus E1B 19 kDa protein interacting protein 3 antibody
BCL2/adenovirus E1B 19 kDa protein-interacting protein 3 antibody
Western blot - Anti-BNIP3 antibody - Carboxyterminal end (ab65874)
Immunocytochemistry/ Immunofluorescence - Anti-BNIP3 antibody - Carboxyterminal end (ab65874)
ICC/IF image of ab65874 stained Mcf7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65874, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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