製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to BNC1
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide conjugated to KLH, corresponding to a region within N terminal amino acids 1-30 of Human BNC1 (NP_001708.3).

  • ポジティブ・コントロール
    • HepG2 cell line lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab123645 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/100 - 1/500. Predicted molecular weight: 111 kDa.

ターゲット情報

  • 機能
    Likely to be a transcription factor specific for squamous epithelium and for the constituent keratinocytes at a stage either prior to or at the very beginning of terminal differentiation. May play a role in the differentiation of spermatozoa and oocytes.
  • 組織特異性
    Expressed in epidermis, testis and foreskin, and to a lower extent in thymus, spleen, mammary glands, placenta, brain and heart.
  • 配列類似性
    Contains 6 C2H2-type zinc fingers.
  • 翻訳後修飾
    Phosphorylation on Ser-537 and Ser-541 leads to cytoplasmic localization.
  • 細胞内局在
    Nucleus. Cytoplasm. Relocates to the midpiece of the flagellum during late spermiogenesis in spermatids.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AI047752 antibody
    • AW546376 antibody
    • basonuclin 1 antibody
    • BNC antibody
    • Bnc1 antibody
    • BNC1_HUMAN antibody
    • BSN1 antibody
    • HsT19447 antibody
    • zinc finger protein basonuclin antibody
    • Zinc finger protein basonuclin-1 antibody
    see all

画像

  • Anti-BNC1 antibody (ab123645) at 1/100 dilution + HepG2 cell line lysate at 35 µg

    Predicted band size : 111 kDa

プロトコール

参考文献

This product has been referenced in:
  • Pangeni RP  et al. The GALNT9, BNC1 and CCDC8 genes are frequently epigenetically dysregulated in breast tumours that metastasise to the brain. Clin Epigenetics 7:57 (2015). WB . Read more (PubMed: 26052355) »

See 1 Publication for this product

レビューと Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Pluripotent stem cell derived epicardial like cell)
Permeabilization
Yes - TritonX
Specification
Pluripotent stem cell derived epicardial like cell
Blocking step
Serum as blocking agent for 15 minute(s) · Concentration: 4% · Temperature: RT°C
Fixative
Paraformaldehyde
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投稿 Jul 13 2016

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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