The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 110 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Likely to be a transcription factor specific for squamous epithelium and for the constituent keratinocytes at a stage either prior to or at the very beginning of terminal differentiation. May play a role in the differentiation of spermatozoa and oocytes.
Expressed in epidermis, testis and foreskin, and to a lower extent in thymus, spleen, mammary glands, placenta, brain and heart.
Contains 6 C2H2-type zinc fingers.
Phosphorylation on Ser-537 and Ser-541 leads to cytoplasmic localization.
Nucleus. Cytoplasm. Relocates to the midpiece of the flagellum during late spermiogenesis in spermatids.