製品の概要

  • 製品名Anti-BMX antibody
    BMX 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to BMX
  • 特異性ab59360 detects endogenous levels of total BMX protein.
  • アプリケーション適用あり: ELISA, ICC/IF, WB, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse
  • 免疫原

    Synthesized non phosphopeptide derived from human BMX around the phosphorylation site of tyrosine 566 (DQYPVS).

  • ポジティブ・コントロール
    • A549 cytoplasmic lysate (WB) Human thyroid gland tissue (IHC)
  • 特記事項Store at -20°C for 1 year

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)Purified from rabbit antiserum by affinity chromatography, using epitope specific immunogen.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab59360 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/10000.
ICC/IF Use at an assay dependent concentration.
WB 1/500 - 1/1000. Detects a band of approximately 78 kDa.
IHC-P Use at an assay dependent concentration.

ターゲット情報

  • 機能Activity is required for interleukin 6 (IL-6) induced differentiation. May play a role in the growth and differentiation of hematopoietic cells. May be involved in signal transduction in endocardial and arterial endothelial cells.
  • 組織特異性Preferentially expressed in epithelial and endothelial cells.
  • 配列類似性Belongs to the protein kinase superfamily. Tyr protein kinase family. TEC subfamily.
    Contains 1 Btk-type zinc finger.
    Contains 1 PH domain.
    Contains 1 protein kinase domain.
    Contains 1 SH2 domain.
  • ドメインSH2 domain mediates interaction with RUFY1.
  • 細胞内局在Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Bmx antibody
    • BMX non receptor tyrosine kinase antibody
    • BMX_HUMAN antibody
    • BONE MARROW KINASE, X-LINKED antibody
    • Bone marrow tyrosine kinase gene in chromosome X protein antibody
    • Cytoplasmic tyrosine protein kinase BMX antibody
    • Cytoplasmic tyrosine-protein kinase BMX antibody
    • Epithelial and endothelial tyrosine kinase antibody
    • ETK antibody
    • NKT38 antibody
    • NTK 38 antibody
    • NTK38 antibody
    • Protein tyrosine kinase BMX antibody
    • PSCTK 2 antibody
    • PSCTK 3 antibody
    • PSCTK2 antibody
    • PSCTK3 antibody
    see all

Anti-BMX antibody 画像

  • All lanes : Anti-BMX antibody (ab59360) at 1/500 dilution

    Lane 1 : Extracts from A549 cells with no immunizing peptide
    Lane 2 : Extracts from A549 cells with immunizing peptide


    Observed band size : 78 kDa (why is the actual band size different from the predicted?)
  • ab59360, at a 1/50 dilution, staining human BMX in thyroid gland, using Immunohistochemistry, Paraffin embedded tissue, in the absence of the immunizing peptide (left image) and in the presence of the immunizing peptide (right image).
  • ICC/IF image of ab59360 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab59360, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-BMX antibody (ab59360) 使用論文

This product has been referenced in:
  • Guryanova OA  et al. Nonreceptor Tyrosine Kinase BMX Maintains Self-Renewal and Tumorigenic Potential of Glioblastoma Stem Cells by Activating STAT3. Cancer Cell 19:498-511 (2011). WB, ICC/IF ; Mouse . Read more (PubMed: 21481791) »

See 1 Publication for this product

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