製品の概要

  • 製品名Anti-BMPR2 antibody [MM0060-9A10]
    BMPR2 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [MM0060-9A10] to BMPR2
  • 特異性ab78422 detects BMPR2. No cross reactivity was found to BMPR1A and BMPR1B.
  • アプリケーション適用あり: WB, Flow Cyt, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant human BMPR2 extracellular domain

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab78422 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/100 - 1/1000. Predicted molecular weight: 115 kDa.
Flow Cyt Use 1-2µg for 106 cells. (methanol or paraformaldehyde fixed cells)



ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-P 1/50 - 1/200. Perform enzymatic antigen retrieval before commencing with IHC staining protocol. We recommend to use Proteinase K for the enzymatic antigen retrieval.

ターゲット情報

  • 機能On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Binds to BMP-7, BMP-2 and, less efficiently, BMP-4. Binding is weak but enhanced by the presence of type I receptors for BMPs.
  • 組織特異性Highly expressed in heart and liver.
  • 関連疾患Defects in BMPR2 are the cause of primary pulmonary hypertension (PPH1) [MIM:178600]. PPH1 is a rare autosomal dominant disorder characterized by plexiform lesions of proliferating endothelial cells in pulmonary arterioles. The lesions lead to elevated pulmonary arterial pression, right ventricular failure, and death. The disease can occur from infancy throughout life and it has a mean age at onset of 36 years. Penetrance is reduced. Although familial PPH1 is rare, cases secondary to known etiologies are more common and include those associated with the appetite-suppressant drugs.
    Defects in BMPR2 are a cause of pulmonary venoocclusive disease (PVOD) [MIM:265450]. PVOD is a rare form of pulmonary hypertension in which the vascular changes originate in the small pulmonary veins and venules. The pathogenesis is unknown and any link with PPH1 has been speculative. The finding of PVOD associated with a BMPR2 mutation reveals a possible pathogenetic connection with PPH1.
  • 配列類似性Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily.
    Contains 1 protein kinase domain.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • BMP type II receptor antibody
    • BMP type-2 receptor antibody
    • BMPR 2 antibody
    • BMPR 3 antibody
    • BMPR II antibody
    • BMPR-2 antibody
    • BMPR-II antibody
    • Bmpr2 antibody
    • BMPR2_HUMAN antibody
    • BMPR3 antibody
    • BMPRII antibody
    • BMR 2 antibody
    • BMR2 antibody
    • Bone morphogenetic protein receptor type 2 antibody
    • Bone morphogenetic protein receptor type II antibody
    • Bone morphogenetic protein receptor type-2 antibody
    • Bone morphogenic protein receptor type II serine threonine kinase antibody
    • BRK 3 antibody
    • BRK3 antibody
    • PPH 1 antibody
    • PPH1 antibody
    • Serine threonine kinase type II activin receptor like kinase antibody
    • T ALK antibody
    • TALK antibody
    • Type II activin receptor like kinase antibody
    see all

Anti-BMPR2 antibody [MM0060-9A10] 画像

  • ab78422 at 1/200 dilution, staining BMPR2 in human placental tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections).

  • Overlay histogram showing HepG2 cells stained with ab78422 (red line). The cells were fixed with methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab78422, 2µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde (10 min) used under the same conditions.

    Please note that Abcam does not have data for use of this antibody on non-fixed cells. We welcome any customer feedback.

Anti-BMPR2 antibody [MM0060-9A10] (ab78422) 使用論文

This product has been referenced in:
  • Huber LC  et al. Featured Article: microRNA-125a in pulmonary hypertension: Regulator of a proliferative phenotype of endothelial cells. Exp Biol Med (Maywood) 240:1580-9 (2015). Read more (PubMed: 25854878) »
  • Jiao G  et al. BMPR2 inhibition induced apoptosis and autophagy via destabilization of XIAP in human chondrosarcoma cells. Cell Death Dis 5:e1571 (2014). Read more (PubMed: 25501832) »

See all 5 Publications for this product

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Thank you for contacting us.

We have used Proteinase K (20ug/ml in PBS) at room temperature for 20-30 minutes as antigen retrieval method. We however recommend customer trying a rage of time e.g. 5, 10, 15 or 20 mins for optimal results.
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Thank you for contacting Abcam.

We recently discussed your experiment double staining with ab28364 and ab78422 via IHC-P. We talked about attempting both an enzymatic (Proteinase K, 37oC) and heat mediated (Citrate buffer, 100oC) antigen retri...

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Thank you for contacting Abcam.

We spoke earlier about two antibodies:
anti-BMPR2 ab78422 (http://www.abcam.com/bmpr2-antibody-mm0060-9a10-ab78422.html)
anti-TRPM8 ab109308 (http://www.abcam.com/trpm8-antibody-epr41962-ab109308.html) Read More

Thank you for your enquiry which has been forwarded to the scientific support team. I am sorry to confirm that because we carry over 70,000 products, it is not feasible for us to keep small sample sizes of our products to send to cusotmers. ...

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Thank you for your inquiry. Please find here the answers to your questions: 1) Proteinase K (20ug/ml) in PBS has been used for antigen retrieval, to pre-treat the tissue section at room temperature for 20-30min 2.) In order to stop the proteinase...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"