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Our Abpromise guarantee covers the use of ab78422 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/100 - 1/1000. Predicted molecular weight: 115 kDa.|
|Flow Cyt||Use 1-2µg for 106 cells. (methanol or paraformaldehyde fixed cells)
ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|IHC-P||1/50 - 1/200. Perform enzymatic antigen retrieval before commencing with IHC staining protocol. We recommend to use Proteinase K for the enzymatic antigen retrieval.|
ab78422 at 1/200 dilution, staining BMPR2 in human placental tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections).