製品の概要

  • 製品名Anti-BMPR1A antibody
    BMPR1A 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to BMPR1A
  • アプリケーション適用あり: IHC-P, IHC-Fr, ICC/IF, WBmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to Human BMPR1A aa 166-195 (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH).

  • ポジティブ・コントロール
    • WB: HeLa and 293 cell lysates. IHC-P: Human hepatocarcinoma tissue. ICC/IF: HeLa cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab38560 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/50 - 1/100.
IHC-Fr Use at an assay dependent concentration. PubMed: 25093411
ICC/IF Use a concentration of 5 µg/ml.
WB 1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).

ターゲット情報

  • 機能On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Receptor for BMP2, BMP4, GDF5 and GDF6. Positively regulates chondrocyte differentiation through GDF5 interaction. Mediates induction of adipogenesis by GDF6.
  • 組織特異性Highly expressed in skeletal muscle.
  • 関連疾患Juvenile polyposis syndrome
    Polyposis syndrome, mixed hereditary 2
    A microdeletion of chromosome 10q23 involving BMPR1A and PTEN is a cause of chromosome 10q23 deletion syndrome, which shows overlapping features of the following three disorders: Bannayan-Zonana syndrome, Cowden disease and juvenile polyposis syndrome.
  • 配列類似性Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily.
    Contains 1 GS domain.
    Contains 1 protein kinase domain.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 10q23del antibody
    • Activin A receptor type II like kinase 3 antibody
    • Activin receptor like kinase 3 antibody
    • Activin receptor-like kinase 3 antibody
    • ACVRLK 3 antibody
    • ACVRLK3 antibody
    • ALK 3 antibody
    • ALK-3 antibody
    • ALK3 antibody
    • BMP type-1A receptor antibody
    • BMPR 1A antibody
    • Bmpr antibody
    • BMPR-1A antibody
    • Bmpr1a antibody
    • BMR1A_HUMAN antibody
    • Bone morphogenetic protein receptor type IA antibody
    • Bone morphogenetic protein receptor type IA precursor antibody
    • Bone morphogenetic protein receptor type-1A antibody
    • BR 1a antibody
    • BR1a antibody
    • CD 292 antibody
    • CD292 antibody
    • CD292 antigen antibody
    • EC 2.7.11.30 antibody
    • Serine threonine protein kinase receptor R5 antibody
    • Serine threonine protein kinase receptor R5 precursor antibody
    • Serine/threonine-protein kinase receptor R5 antibody
    • SKR 5 antibody
    • SKR5 antibody
    • zBMPR IA antibody
    • zBMPRIA antibody
    see all

Anti-BMPR1A antibody 画像

  • All lanes : Anti-BMPR1A antibody (ab38560) at 1000 cells

    Lane 1 : HEK293 cell line
    Lane 2 : Mouse NIH/3T3 cell line

    Lysates/proteins at 35 mg/ml per lane.

    Secondary
    Goat anti-rabbit IgG H&L(HRP) at 1/5000 dilution

    Predicted band size : 60 kDa
  • Anti-BMPR1A antibody (ab38560) at 1/1000 dilution + Hela cell line lysates at 35 µg

    Predicted band size : 60 kDa
  • Immunohistochemical (formalin/PFA-fixed paraffin-embedded sections) analysis of human hepatocarcinoma tissue labelling BMPR1A with ab38560. Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at 38°C. Antigen retrieval was heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A peroxidase-conjugated goat anti-rabbit polyclonal (ready to use) was used as the secondary antibody.

  • ICC/IF image of ab38560 stained HeLa cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab38560, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-BMPR1A antibody (ab38560) 使用論文

This product has been referenced in:
  • Yuan S  et al. Post-transcriptional Regulation of Keratinocyte Progenitor Cell Expansion, Differentiation and Hair Follicle Regression by miR-22. PLoS Genet 11:e1005253 (2015). Read more (PubMed: 26020521) »
  • Gu S  et al. BMPRIA mediated signaling is essential for temporomandibular joint development in mice. PLoS One 9:e101000 (2014). IHC-Fr ; Mouse . Read more (PubMed: 25093411) »

See all 3 Publications for this product

Product Wall

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - whole cell (HEK293 cells)
Specification HEK293 cells
Treatment FLAG, or FLAG-BMPR1A transfected
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
Username

Abcam user community

Verified customer

投稿 Mar 11 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - whole (Rat aorta)
Loading amount 40 µg
Specification Rat aorta
Gel Running Conditions Reduced Denaturing (9% SDS-PAGE)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Chrispina Hong

Verified customer

投稿 Jan 18 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (intestine)
Specification intestine
Fixative Paraformaldehyde
Permeabilization Yes - .01% triton x-100
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

投稿 Apr 17 2012



I am very pleased to hear you would like to accept our offer and test ab38560 in chicken. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview forchicken and include this...

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