The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/500. Detects a band of approximately 50 kDa (predicted molecular weight: 47 kDa).
1/50 - 1/100.
BMP9 is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. They are regulators of cell growth and differentiation.
BMP9 has a role in the maturation of BFCN (basal forebrain cholinergic neurons) also inducing and maintaining the ability of these cells to respond to acetylcholine; BFCN are important for the processes of learning, memory and attention. BMP9 is a potent inducer of hepcidin (a cationic peptide that has a antimicrobial properties) in hepatocytes and can regulate iron metabolism. The physiological receptor of BMP9 is thought to be activin receptor-like kinase 1, ALK1 (also called ACVRL1), an endothelial-specific type I receptor of the TGF-beta receptor family.
BMP9 is one of the most potent BMPs to induce orthotopic bone formation in vivo. BMP3, a blocker of most BMPs seems not to affect BMP9.
ab71809 at 1/50 dilution, staining BMP9 in human hepatocarcinoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue, followed by peroxidase-conjugated secondary antibody and DAB staining.