製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to BMP9
  • アプリケーション
    適用あり: ELISA, IHC-P, ICC/IF, WB, IHC-Frmore details
  • 種交差性
    交差種: Rat, Human
    交差が予測される動物種: Mouse, Dog, Chimpanzee, Macaque monkey
  • 免疫原

    Synthetic peptide:

    CKDDMGVPTLKYH

    , corresponding to amino acids 87-98 of Human BMP9 (corresponding to amino acids 406-417 of the full length propeptide)

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab35088 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/50 - 1/200.
IHC-P Use at an assay dependent concentration.
ICC/IF 1/500.
WB Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration. PubMed: 20065063

ターゲット情報

  • 関連性
    BMP9 is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. They are regulators of cell growth and differentiation. BMP9 has a role in the maturation of BFCN (basal forebrain cholinergic neurons) also inducing and maintaining the ability of these cells to respond to acetylcholine; BFCN are important for the processes of learning, memory and attention. BMP9 is a potent inducer of hepcidin (a cationic peptide that has a antimicrobial properties) in hepatocytes and can regulate iron metabolism. The physiological receptor of BMP9 is thought to be activin receptor-like kinase 1, ALK1 (also called ACVRL1), an endothelial-specific type I receptor of the TGF-beta receptor family. BMP9 is one of the most potent BMPs to induce orthotopic bone formation in vivo. BMP3, a blocker of most BMPs seems not to affect BMP9.
  • 細胞内局在
    Secreted
  • 参照データベース
  • 別名
    • BMP 9 antibody
    • Bone morphogenetic protein 9 antibody
    • GDF 2 antibody
    • GDF2 antibody
    • growth differentiation factor 2 antibody
    • Growth/differentiation factor 2 antibody
    • HHT5 antibody
    see all

Anti-BMP9 antibody 画像

  • ab35088 (1/100) staining BMP9 in paraffin-embbeded human lung vascular lesion tissue sections. Tissue underwent fixation in formaldehyde, heat-mediated antigen retrieval in citrate buffer. For further experimental details please refer to abreview.

    See Abreview

  • Immunofluorescent staining of BMP9 in rat primary hippocampus neurons with ab35088 (1/500). Cells were fixed with PFA and permeabilized with 0.3% Triton X in 0.1% PBS followed by a blocking step with 10% serum for 1 hour at 24oC.  Incubation with ab35088 (1/500) was then performed during 2 hours at 24oC. The secondary antibody was an anti rabbit-Alexa Fluro 488. DAPI shows nuclear localization.

    See Abreview

Anti-BMP9 antibody (ab35088) 使用論文

This product has been referenced in:
  • Voorneveld PW  et al. The BMP pathway either enhances or inhibits the Wnt pathway depending on the SMAD4 and p53 status in CRC. Br J Cancer 112:122-30 (2015). Read more (PubMed: 25393365) »
  • Cunha SI  et al. Endothelial ALK1 Is a Therapeutic Target to Block Metastatic Dissemination of Breast Cancer. Cancer Res 75:2445-56 (2015). Read more (PubMed: 26077471) »

See all 5 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Tissue lysate - whole (Liver)
Gel Running Conditions
Reduced Denaturing (9% SDS-PAGE)
Loading amount
20 µg
Specification
Liver
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Abcam user community

Verified customer

投稿 Apr 19 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Primary Hippocampal culture)
Specification
Primary Hippocampal culture
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.3% TritonX in 0.1% PBS
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Username

Dr. Ruma Raha-Chowdhury

Verified customer

投稿 Apr 04 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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