製品の概要

  • 製品名
    Anti-BMP6 antibody [morph-6.1]
    BMP6 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [morph-6.1] to BMP6
  • アプリケーション
    適用あり: IHC-P, ICC/IF, WBmore details
  • 種交差性
    交差種: Mouse, Rat, Dog, Human
  • 免疫原

    Synthetic peptide:

    MPGLGRRAQWLCWWWGLLCSCCGPPPLRP

    conjugated to KLH, corresponding to amino acids 1-29 of Rat BMP6.

  • ポジティブ・コントロール
    • Positive staining can be observed in embryonic and adult central nervous system. Rat radial glial cells of the developing central nervous system from E11 to E19. In rat peripheral nerves a selective intracellular immunoreactivity can be found in perinuclear region of most Schwann cells which form the myelin sheath. However, some Schwann cells were negative for the antigen.
  • 特記事項


    Pre-incubation of anti BMP6 with amino-terminal peptide 1-29 of BMP6 inhibits binding of the mAb to tissue sections. Pre-incubation with other amino-terminal synthetic peptides of human BMP7, BMP4, BMP3 and BMP2 as well as an irrelevant peptide does not inhibit specific tissue staining.

法規制情報

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab15640 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 µg/ml.
WB 1/100. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).

ターゲット情報

画像

  • ab15640 staining human U2-OS cells by ICC/IF. Cells were PFA fixed and permeabilized in Triton-X-100 prior to blocking in 2% serum for 1 hour at 20°C. The primary antibody was diluted 1/100 and incubated with the sample for 1 hour at 37°C. The secondary antibody was Alexa Fluor®488 conjugated chicken anti-mouse, diluted 1/1000.

    See Abreview

  • All lanes : Anti-BMP6 antibody [morph-6.1] (ab15640) at 1/400 dilution

    Lane 1 : mouse liver
    Lane 2 : mouse liver
    Lane 3 : mouse liver
    Lane 4 : mouse liver

    Lysates/proteins at 20 µg per lane.

    Secondary
    Non-Abcam Antibody was used: Dako Cytomation P0260 Conjugation: HRP at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 57 kDa
    Observed band size : 57 kDa
    Additional bands at : 25&30&75 kDa (possible non-specific binding).

    Exposure time : 10 seconds

    This image is courtesy of an Abreview submitted by Dr Ruma Raha-Chowdhury

    See Abreview

  • ICC/IF image of ab15640 staining primary myoblast cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 5% normal donkey serum / 0.3M glycine in 0.1% PBS-Tritonx100 for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab15640, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488donkey anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h.

参考文献

This product has been referenced in:
  • Garrison P  et al. Spatial regulation of bone morphogenetic proteins (BMPs) in postnatal articular and growth plate cartilage. PLoS One 12:e0176752 (2017). IHC ; Mouse . Read more (PubMed: 28467498) »
  • Díaz PU  et al. BMP2, 4 and 6 and BMPR1B are altered from early stages of bovine cystic ovarian disease development. Reproduction 152:333-50 (2016). Cow . Read more (PubMed: 27486268) »

See all 4 Publications for this product

レビューと Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Cartilage)
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: Proteinase K 100ug/ml
Permeabilization
No
Specification
Cartilage
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1.5% · Temperature: 24°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

投稿 Dec 02 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Myoblast)
Specification
Myoblast
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.3% Triton X
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Dr. Ruma Raha-Chowdhury

Verified customer

投稿 Dec 14 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Liver)
Loading amount
20 µg
Specification
Liver
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 4°C
Username

Dr. Ruma Raha-Chowdhury

Verified customer

投稿 Jun 02 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (U2-OS)
Specification
U2-OS
Fixative
Paraformaldehyde
Permeabilization
Yes - Triton-X-100
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 20°C
Username

Abcam user community

Verified customer

投稿 Nov 17 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Cell lysate - whole cell (Schwannom cell line RN 22)
Loading amount
25 µg
Specification
Schwannom cell line RN 22
Gel Running Conditions
Reduced Denaturing (15% SDS)
Blocking step
Odyssey Blocking Buffer 1:1 diluted with PBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: 20°C
Username

Abcam user community

Verified customer

投稿 Feb 19 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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