Positive staining can be observed in embryonic and adult central nervous system. Rat radial glial cells of the developing central nervous system from E11 to E19. In rat peripheral nerves a selective intracellular immunoreactivity can be found in perinuclear region of most Schwann cells which form the myelin sheath. However, some Schwann cells were negative for the antigen.
Pre-incubation of anti BMP6 with amino-terminal peptide 1-29 of BMP6 inhibits binding of the mAb to tissue sections. Pre-incubation with other amino-terminal synthetic peptides of human BMP7, BMP4, BMP3 and BMP2 as well as an irrelevant peptide does not inhibit specific tissue staining.
保存方法Shipped at 4°C. Upon delivery aliquot. Avoid freeze / thaw cycle. Stable for 12 months at -20°C.
バッファーPreservative: 0.01% Thimerosal (merthiolate)
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一次抗体 備考Pre-incubation of anti BMP6 with amino-terminal peptide 1-29 of BMP6 inhibits binding of the mAb to tissue sections. Pre-incubation with other amino-terminal synthetic peptides of human BMP7, BMP4, BMP3 and BMP2 as well as an irrelevant peptide does not inhibit specific tissue staining.
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Vg1 related sequence antibody
Anti-BMP6 antibody [morph-6.1] 画像
Immunocytochemistry/ Immunofluorescence - Anti-BMP6 antibody [morph-6.1] (ab15640)This image is courtesy of an anonymous Abreview
ab15640 staining human U2-OS cells by ICC/IF. Cells were PFA fixed and permeabilized in Triton-X-100 prior to blocking in 2% serum for 1 hour at 20°C. The primary antibody was diluted 1/100 and incubated with the sample for 1 hour at 37°C. The secondary antibody was Alexa Fluor®488 conjugated chicken anti-mouse, diluted 1/1000.
Immunocytochemistry/ Immunofluorescence - Anti-BMP6 antibody [morph-6.1] (ab15640)This image is courtesy of Ruma Raha-Chowdhury, University Of Cambridge, United Kingdom
ICC/IF image of ab15640 staining primary myoblast cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 5% normal donkey serum / 0.3M glycine in 0.1% PBS-Tritonx100 for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab15640, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488donkey anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h.
Anti-BMP6 antibody [morph-6.1] (ab15640) 使用論文
This product has been referenced in:
Garrison P et al. Spatial regulation of bone morphogenetic proteins (BMPs) in postnatal articular and growth plate cartilage. PLoS One12:e0176752 (2017).
Read more (PubMed: 28467498) »
Díaz PU et al. BMP2, 4 and 6 and BMPR1B are altered from early stages of bovine cystic ovarian disease development. Reproduction152:333-50 (2016).
Read more (PubMed: 27486268) »