Positive staining can be observed in embryonic and adult central nervous system. Rat radial glial cells of the developing central nervous system from E11 to E19. In rat peripheral nerves a selective intracellular immunoreactivity can be found in perinuclear region of most Schwann cells which form the myelin sheath. However, some Schwann cells were negative for the antigen.
Pre-incubation of anti BMP6 with amino-terminal peptide 1-29 of BMP6 inhibits binding of the mAb to tissue sections. Pre-incubation with other amino-terminal synthetic peptides of human BMP7, BMP4, BMP3 and BMP2 as well as an irrelevant peptide does not inhibit specific tissue staining.
保存方法Shipped at 4°C. Upon delivery aliquot. Avoid freeze / thaw cycle. Stable for 12 months at -20°C.
バッファーPreservative: 0.01% Thimerosal (merthiolate)
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一次抗体 備考Pre-incubation of anti BMP6 with amino-terminal peptide 1-29 of BMP6 inhibits binding of the mAb to tissue sections. Pre-incubation with other amino-terminal synthetic peptides of human BMP7, BMP4, BMP3 and BMP2 as well as an irrelevant peptide does not inhibit specific tissue staining.
TGFB related vegetal related growth factor antibody
Transforming growth factor beta antibody
Vegetal related (TGFB related) cytokine antibody
Vegetal related growth factor (TGFB related) antibody
Vg related sequence antibody
VG-1-related protein antibody
Vg1 related sequence antibody
Anti-BMP6 antibody [morph-6.1] 画像
Immunocytochemistry/ Immunofluorescence - BMP6 antibody [morph-6.1] (ab15640)This image is courtesy of an anonymous Abreview
ab15640 staining human U2-OS cells by ICC/IF. Cells were PFA fixed and permeabilized in Triton-X-100 prior to blocking in 2% serum for 1 hour at 20°C. The primary antibody was diluted 1/100 and incubated with the sample for 1 hour at 37°C. The secondary antibody was Alexa Fluor®488 conjugated chicken anti-mouse, diluted 1/1000.
Immunocytochemistry/ Immunofluorescence - Anti-BMP6 antibody [morph-6.1] (ab15640)This image is courtesy of Ruma Raha-Chowdhury, University Of Cambridge, United Kingdom
ICC/IF image of ab15640 staining primary myoblast cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 5% normal donkey serum / 0.3M glycine in 0.1% PBS-Tritonx100 for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab15640, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488donkey anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h.
Anti-BMP6 antibody [morph-6.1] (ab15640) 使用論文
This product has been referenced in:
Wensman H et al. Diverse bone morphogenetic protein expression profiles and smad pathway activation in different phenotypes of experimental canine mammary tumors. PLoS One4:e7133 (2009).
Read more (PubMed: 19771160) »
Jung Y et al. Hematopoietic stem cells regulate mesenchymal stromal cell induction into osteoblasts thereby participating in the formation of the stem cell niche. Stem Cells26:2042-51 (2008).
Read more (PubMed: 18499897) »