ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
For unpurified use at 1/50 - 1/100.
Induces cartilage and bone formation. Also act in mesoderm induction, tooth development, limb formation and fracture repair. Acts in concert with PTHLH/PTHRP to stimulate ductal outgrowth during embryonic mammary development and to inhibit hair follicle induction.
Expressed in the lung and lower levels seen in the kidney. Present also in normal and neoplastic prostate tissues, and prostate cancer cell lines.
Defects in BMP4 are the cause of microphthalmia syndromic type 6 (MCOPS6) [MIM:607932]; also known as microphthalmia and pituitary anomalies or microphthalmia with brain and digit developmental anomalies. Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues (anophthalmia). In many cases, microphthalmia/anophthalmia occurs in association with syndromes that include non-ocular abnormalities. MCOPS6 is characterized by microphthalmia/anophthalmia associated with facial, genital, skeletal, neurologic and endocrine anomalies. Defects in BMP4 are the cause of non-syndromic orofacial cleft type 11 (OFC11) [MIM:600625]. Non-syndromic orofacial cleft is a common birth defect consisting of cleft lips with or without cleft palate. Cleft lips are associated with cleft palate in two-third of cases. A cleft lip can occur on one or both sides and range in severity from a simple notch in the upper lip to a complete opening in the lip extending into the floor of the nostril and involving the upper gum. OFC11 is an unusual anomaly consisting of a paramedian scar of the upper lip with an appearance suggesting that a typical cleft lip was corrected in utero.
Belongs to the TGF-beta family.
Secreted > extracellular space > extracellular matrix.
ab124715 (purified) at 1:60 dilution (2µg) immunoprecipitating BMP4 in A549 whole cell lysate. Lane 1 (input): A549 (Human lung carcinoma epithelial cell) whole cell lysate 10µg Lane 2 (+): ab124715 & A549 whole cell lysate Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab124715 in A549 whole cell lysate For western blotting, VeriBlot for IP secondary antibody (HRP) (ab131366) was used as the secondary antibody at 1:1000 dilution. Blocking and diluting buffer: 5% NFDM/TBST.
Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling BMP4 with purified ab124715 at 1:120 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
Immunocytochemistry/Immunofluorescence analysis of A549 (human lung carcinoma) cells labelling BMP4 with purified ab124715 at 1/500. Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Nuclei were counterstained with DAPI (blue).
Secondary Only Control: PBS was used instead of the primary antibody as the negative control.
Western blot - Anti-BMP4 antibody [EPR6211] (ab124715)
All lanes : Anti-BMP4 antibody [EPR6211] (ab124715) at 1/10000 dilution (Unpurified)
Lane 1 : Human fetal liver lysate Lane 2 : A549 lysate Lane 3 : HeLa lysate Lane 4 : A431 lysate Lane 5 : Human heart lysate
Lysates/proteins at 10 µg per lane.
Secondary All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 47 kDa
Other - Anti-BMP4 antibody [EPR6211] (ab124715)
Produced using unpurified ab124715
Equilibrium disassociation constant (KD) Learn more about KD