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Our Abpromise guarantee covers the use of ab14933 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 45 kDa.Can be blocked with Mouse BMP2 peptide (ab80798).|
|ICC/IF||Use a concentration of 1 µg/ml.|
|IHC-Fr||1/250 - 1/500.|
|IHC-P||Use at an assay dependent concentration.|
ab14933 at 1/200 dilution staining BMP2 in human 293FT cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed in formaldehyde and blocked in 5% BSA for 1 hour at 25°C. The primary antibody was used at 1/200 dilution in PBS and incubated with sample at 4°C for 12 hours. An Alexa Fluor® 488 conjugated Goat polyclonal to rabbit IgG was used as secondary at 1/200 dilution.Cells were cultured in DMEM containing 10% FBS and Nuclei stained with DAPI.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"