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RabMAb

Anti-BMP11 + GDF8 / Myostatin 抗体 [EPR4567(2)] (ab124721)

製品の概要

  • 製品名
    Anti-BMP11 + GDF8 / Myostatin antibody [EPR4567(2)]
  • 製品の詳細
    Rabbit monoclonal [EPR4567(2)] to BMP11 + GDF8 / Myostatin
  • アプリケーション
    適用あり: Flow Cyt, ICC/IF, WB, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human BMP11 + GDF8/ Myostatin aa 350 to the C-terminus.
    Database link: O95390

  • ポジティブ・コントロール
    • WB: HeLa, HEK293 and C6 cell lysate and human heart tissue lysate. C2C12 cell lysate. IHC-P: Human colon adenocarcinoma and cerebral cortex tissues. ICC/IF: A549 cells.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab124721 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use at an assay dependent concentration.

Purified format.

ICC/IF 1/250.
WB 1/1000 - 1/2000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
IHC-P 1/1000 - 1/2500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See protocols (link: http://www.abcam.com/protocols/ihc-antigen-retrieval-protocol).

For unpurified use at 1/500 - 1/1000.

ターゲット情報

Anti-BMP11 + GDF8 / Myostatin antibody [EPR4567(2)] 画像

  • Anti-BMP11 + GDF8 / Myostatin antibody [EPR4567(2)] (ab124721) at 1/1000 dilution + C2C12 (mouse myoblast) whole cell lysates at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size : 45 kDa
    Additional bands at : 45 kDa. We are unsure as to the identity of these extra bands.

    Blocking and Diluting buffer: 5% NFDM /TBST

  • Anti-BMP11 + GDF8 / Myostatin antibody [EPR4567(2)] (ab124721) at 1/1000 dilution (purified) + HEK293 cell lysate at 20 µg

    Secondary
    Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size : 45 kDa
    Observed band size : 45 kDa

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon adenocarcinoma tissue labelling BMP11 + GDF8/Myostatin with unpurified ab124721 at 1/500 dilution.

  • ab124721 staining BMP11 + GDF8 / Myostatin in the human cell line HeLa (human cervix adenocarcinoma) by flow cytometry. Cells were fixed with 4% paraformaldehyde, permabilised with 90% methanol and the sample was incubated with the primary antibody at a dilution of 1/250. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/2000 was used as the secondary antibody.

    Isoytype control: Rabbit monoclonal IgG (Black)

    Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue)

  • Anti-BMP11 + GDF8 / Myostatin antibody [EPR4567(2)] (ab124721) at 1/5000 dilution (purified) + HeLa cell lysate at 10 µg

    Secondary
    Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size : 45 kDa
    Observed band size : 45 kDa

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Anti-BMP11 + GDF8 / Myostatin antibody [EPR4567(2)] (ab124721) at 1/1000 dilution (purified) + C6 cell lysate at 20 µg

    Secondary
    Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size : 45 kDa
    Observed band size : 45 kDa

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • All lanes : Anti-BMP11 + GDF8 / Myostatin antibody [EPR4567(2)] (ab124721) at 1/1000 dilution (unpurified)

    Lane 1 : HeLa cell lysate
    Lane 2 : Human heart tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

    Predicted band size : 45 kDa
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebral cortex tissue labelling BMP11 + GDF8/Myostatin with purified ab124721 at 1/2500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  • Immunocytochemistry/Immunofluorescence analysis of A549 cells labelling BMP11 + GDF8/Myostatin with purified ab124721 at 1/250. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% triton X-100. ab150078, an Alexa Fluor® 555-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain.

    Control: primary antibody (1/250) and secondary antibody, ab150113, an Alexa Fluor® 488-conjugated goat anti-mouse IgG (1/500).

  • Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

Anti-BMP11 + GDF8 / Myostatin antibody [EPR4567(2)] (ab124721) 使用論文

This product has been referenced in:

See all 5 Publications for this product

Product Wall

Application
ELISA
Sample
Mouse Serum (heparin tubes collected whole blood and then spun)
Specification
heparin tubes collected whole blood and then spun
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Type
Sandwich (Capture)
Username

Abcam user community

Verified customer

投稿 Sep 14 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (liver)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer
Permeabilization
No
Specification
liver
Blocking step
Serum as blocking agent for 15 minute(s) · Concentration: 20% · Temperature: 20°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

投稿 Aug 13 2015

Application
Western blot
Sample
Mouse Tissue lysate - whole (Mouse liver + kidney + heart & human Hela cells)
Gel Running Conditions
Reduced Denaturing (15)
Loading amount
100 µg
Treatment
Halt Phosphatase inhibitor cocktail before lysis
Specification
Mouse liver + kidney + heart & human Hela cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Selina Moebus

Verified customer

投稿 May 28 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Permeabilization
No
Fixative
Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

投稿 Jul 25 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Sample
Rat Tissue sections (Skin and DRG in situ)
Specification
Skin and DRG in situ
Permeabilization
No
Fixative
Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

投稿 Jul 25 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Sample
Human Tissue sections (Testis)
Specification
Testis
Permeabilization
No
Fixative
Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

投稿 Jul 25 2013


I have compared the immunogen of ab124721 with myostatin and there is an 80% homology so there are chances that this antibodies also recognises Myostatin however, we have not specifically tested this so can't be sure.
I have compared the tota...

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Thank you for contacting us. We don't determine the exact MW of the antibodies themselves, but I can ell you that antibodies are generally 140 kD.

The MW of the target BMP11 is 45 Kd, which is the size it has come up on our WB.

I hope...

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Thank you for your reply. Yes, we do recommend storing this antibody at -20C and it should be stable for at least 12 months from the date of purchase. Please let me know if you need any additional information or assistance.

Merci de votre émail.

Nous n'avons pas encore testé si le ab71347 peux réagir avec la séquence de souris. En regardant l'alignement de la séquence de la protéine humaine avec la séquence de la ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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