製品の概要

  • 製品名Anti-BMP11 antibody
    BMP11 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to BMP11
  • アプリケーション適用あり: IHC-P, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide corresponding to Human BMP11 aa 32-61 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: 10220

  • ポジティブ・コントロール
    • WB: 293 cell lysate. IHC-P: human colon carcinoma tissue.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • 精製度Ammonium Sulphate Precipitation
  • 特記事項(精製)This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab71347 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/10 - 1/50.
WB 1/2000. Detects a band of approximately 55 kDa (predicted molecular weight: 45 kDa).

ターゲット情報

  • 機能Secreted signal that acts globally to specify positional identity along the anterior/posterior axis during development. Play critical roles in patterning both mesodermal and neural tissues and in establishing the skeletal pattern.
  • 配列類似性Belongs to the TGF-beta family.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • BMP 11 antibody
    • BMP-11 antibody
    • BMP11 antibody
    • Bone morphogenetic protein 11 antibody
    • GDF 11 antibody
    • GDF-11 antibody
    • Gdf11 antibody
    • GDF11_HUMAN antibody
    • Growth differentiation factor 11 antibody
    • Growth/differentiation factor 11 antibody
    see all

Anti-BMP11 antibody 画像

  • All lanes : Anti-BMP11 antibody (ab71347) at 1/1000 dilution

    Lane 1 : 293 cell lysate, non transfected
    Lane 2 : 293 cell lysate, transiently transfected with the BMP11 gene

    Lysates/proteins at 2 µg per lane.

    Secondary
    Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/5000 dilution

    Predicted band size : 45 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)

    Incubation time was overnight at 4°C. Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue labelling BMP11 with ab71347. Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at 38°C; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hour at 37°C. A peroxidase-conjugated goat anti-rabbit polyclonal (ready to use) was used as the secondary antibody.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue labelling BMP11 with ab71347. Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at 38°C; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hour at 37°C. A peroxidase-conjugated goat anti-rabbit polyclonal (ready to use) was used as the secondary antibody.

Anti-BMP11 antibody (ab71347) 使用論文

ab71347 has not yet been referenced specifically in any publications.

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I have gathered the following information:


ab124721: The immunogen range is located within the mature human protein (aa 299 - 407).


ab56645:immunogne is a recombinant fragment: ESRC...

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