Anti-Bmi1 抗体 (ab85688)
Key features and details
- Rabbit polyclonal to Bmi1
- Suitable for: ICC/IF, WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-Bmi1 antibody
Bmi1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Bmi1 -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WB, IP, IHC-Pmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis, Platypus -
免疫原
Synthetic peptide, corresponding to a region between amino acids 276-326 of Human Bmi1 (NP_005171.4)
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab85688の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
Use at an assay dependent concentration.
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WB |
1/2000 - 1/10000. Predicted molecular weight: 37 kDa.
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IP |
Use at 2-5 µg/mg of lysate.
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IHC-P | (1) |
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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特記事項 |
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ICC/IF
Use at an assay dependent concentration. |
WB
1/2000 - 1/10000. Predicted molecular weight: 37 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2. -
配列類似性
Contains 1 RING-type zinc finger. -
翻訳後修飾
Monoubiquitinated (By similarity). May be polyubiquitinated; which does not lead to proteasomal degradation. -
細胞内局在
Nucleus. Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 493647 Chicken
- Entrez Gene: 510666 Cow
- Entrez Gene: 100532731 Human
- Entrez Gene: 648 Human
- Entrez Gene: 12151 Mouse
- Entrez Gene: 100173052 Orangutan
- Entrez Gene: 307151 Rat
- Omim: 164831 Human
see all -
別名
- B lymphoma Mo MLV insertion region (mouse) antibody
- B lymphoma Mo MLV insertion region 1 homolog antibody
- Bmi 1 antibody
see all
画像
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All lanes : Anti-Bmi1 antibody (ab85688) at 0.1 µg/ml
Lane 1 : HeLa cell lysate at 50 µg
Lane 2 : HeLa cell lysate at 15 µg
Lane 3 : HeLa cell lysate at 5 µg
Lane 4 : 293T cell lysate at 50 µg
Lane 5 : NIH3T3 cell lysate at 50 µg
Predicted band size: 37 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?
Additional bands at: 140 kDa, 55 kDa. We are unsure as to the identity of these extra bands. -
Detection of Human Bmi1 by mmunoprecipitation. Sample: HeLa Whole cell lysate (1 mg for IP, 20% of IP loaded). ab85688 at 3 ug/mg lysate was used for IP, and at 1ug/ml for subsequent WB detection.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue labelling Bmi1 with ab85688 at 1/1000 (1µg/ml). Detection: DAB.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (7)
ab85688 は 7 報の論文で使用されています。
- Sebestyén E et al. SAMMY-seq reveals early alteration of heterochromatin and deregulation of bivalent genes in Hutchinson-Gilford Progeria Syndrome. Nat Commun 11:6274 (2020). PubMed: 33293552
- Yang W et al. BMI1 promotes cardiac fibrosis in ischemia-induced heart failure via the PTEN-PI3K/Akt-mTOR signaling pathway. Am J Physiol Heart Circ Physiol 316:H61-H69 (2019). PubMed: 30359076
- Wang W et al. B-cell specific Moloney leukemia virus insert site 1 and peptidyl arginine deiminase IV positively regulate carcinogenesis and progression of esophageal squamous cell carcinoma. Oncol Lett 13:4349-4356 (2017). PubMed: 28599437
- Casa V et al. Polycomb repressive complex 1 provides a molecular explanation for repeat copy number dependency in FSHD muscular dystrophy. Hum Mol Genet 26:753-767 (2017). WB, ChIP . PubMed: 28040729
- Ji H et al. Expression and Clinicopathological Significance of Mel-18 and Bmi-1 in Esophageal Squamous Cell Carcinoma. Technol Cancer Res Treat N/A:1533034617705055 (2017). PubMed: 28425347
- Fang L et al. Jumonji AT-rich interactive domain 1B overexpression is associated with the development and progression of glioma. Int J Mol Med 38:172-82 (2016). WB ; Human . PubMed: 27246838
- Qu D et al. Ablation of Doublecortin-Like Kinase 1 in the Colonic Epithelium Exacerbates Dextran Sulfate Sodium-Induced Colitis. PLoS One 10:e0134212 (2015). IHC ; Mouse . PubMed: 26285154