製品の概要

  • 製品名Anti-Bmi1 antibody
    Bmi1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Bmi1
  • アプリケーション適用あり: ICC/IF, IHC-P, WB, IHC-Fr, IPmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to 15 amino acids near the center of human Bmi1.

  • ポジティブ・コントロール
    • K562 cell lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab38295 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 10 µg/ml.
IHC-P Use at an assay dependent concentration. PubMed: 20083111
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 37 kDa.Can be blocked with Human Bmi1 peptide (ab39733).
IHC-Fr 1/300.
IP 1/2000.

ターゲット情報

  • 機能Component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2.
  • 配列類似性Contains 1 RING-type zinc finger.
  • 翻訳後修飾Monoubiquitinated (By similarity). May be polyubiquitinated; which does not lead to proteasomal degradation.
  • 細胞内局在Nucleus. Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • B lymphoma Mo MLV insertion region (mouse) antibody
    • B lymphoma Mo MLV insertion region 1 homolog antibody
    • Bmi 1 antibody
    • BMI1 antibody
    • BMI1 polycomb ring finger oncogene antibody
    • BMI1_HUMAN antibody
    • Flvi 2/bmi 1 antibody
    • FLVI2/BMI1 antibody
    • MGC12685 antibody
    • Murine leukemia viral (bmi 1) oncogene homolog antibody
    • Oncogene BMI 1 antibody
    • PCGF 4 antibody
    • PCGF4 antibody
    • Polycomb complex protein BMI 1 antibody
    • Polycomb complex protein BMI-1 antibody
    • Polycomb group protein Bmi1 antibody
    • Polycomb group ring finger 4 antibody
    • Polycomb group RING finger protein 4 antibody
    • RING finger protein 51 antibody
    • RNF 51 antibody
    • RNF51 antibody
    see all

Anti-Bmi1 antibody 画像

  • ab38295 staining Bmi1 in Rat brain tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde and blocked with 5% serum for 1 hour at 25°C. Samples were incubated with primary antibody (1/300) for 10 hours at 4°C. An Alexa Fluor® 555-conjugated Donkey anti-rabbit IgG polyclonal (1/300) was used as the secondary antibody.

    See Abreview

  • ab38295 staining Bmi1 in Rat fibroblast cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.01% Triton X-100 and blocked with 2.5% Goat serum for 10 minutes at 25°C. Samples were incubated with primary antibody (1/300) for 2 hour at 25°C. An Alexa Fluor® 555-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.

    See Abreview

  • Lane 1 : Anti-Bmi1 antibody (ab38295) at 0.5 µg/ml
    Lane 2 : Anti-Bmi1 antibody (ab38295) at 1 µg/ml
    Lane 3 : Anti-Bmi1 antibody (ab38295) at 2 µg/ml

    Lane 1 : K562 cell lysate
    Lane 2 : K562 cell lysate
    Lane 3 : K562 cell lysate


    Predicted band size : 37 kDa
    Observed band size : 33 kDa (why is the actual band size different from the predicted?)
  • Immunofluorescence of BMI-1 in K562 cells using ab38295 at 20 ug/ml.

Anti-Bmi1 antibody (ab38295) 使用論文

This product has been referenced in:
  • Fu TG  et al. miR-143 inhibits oncogenic traits by degrading NUAK2 in glioblastoma. Int J Mol Med 37:1627-35 (2016). WB ; Human . Read more (PubMed: 27081712) »
  • Szabo AZ  et al. The CD44(+) ALDH(+) Population of Human Keratinocytes is Enriched for Epidermal Stem Cells with Long Term Repopulating Ability. Stem Cells : (2013). Read more (PubMed: 23335266) »

See all 11 Publications for this product

Product Wall

Application Immunoprecipitation
Immuno-precipitation step Protein A/G
Sample Rat Cell lysate - whole cell (Oligodendrocytes)
Specification Oligodendrocytes
Total protein in input 30 µg
Username

Abcam user community

Verified customer

投稿 Dec 09 2013

Application Western blot
Loading amount 40 µg
Gel Running Conditions Reduced Non-Denaturing (Native) (12.5%)
Sample Rat Cell lysate - whole cell (Oligodendrocyte)
Specification Oligodendrocyte
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Dec 09 2013

Application Immunocytochemistry
Blocking step Serum as blocking agent for 10 minute(s) · Concentration: 2.5% · Temperature: 25°C
Sample Rat Cell (Fibroblast)
Specification Fibroblast
Permeabilization Yes - 0.01% triton X100
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Oct 30 2013

Application Immunohistochemistry (Frozen sections)
Sample Rat Tissue sections (Brain)
Specification Brain
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Oct 28 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (MKN45)
Loading amount 30 µg
Specification MKN45
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 May 14 2013

Thank you for your response.
I have discussed this enquiry with my colleagues.
A BLAST with the immunogen in the human proteome does not predict cross-reactivity with any other proteins, just BMI-1 and partial BMI-1 sequences. It could be a c...

Read More

Thank you for your enquiry regarding ab38295 and for taking the time to provide some useful details of the experiments. I am very sorry to hear that you are having problems with this antibody.
My colleague has asked me to look after her customers ...

Read More

Vielen Dank für Ihre Anfrage.
Es tut mir Leid zu hören, dass Sie Probleme mit diesem Antikörper haben. Laut unseren Unterlagen haben wir den Antikörper nicht selber in der IHC getestet, sondern haben diese Applikation aufgrund einer Publikation au...

Read More

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacementcontaining 1 vialeach of ab38237,13533,38295.

To check the status of the or...

Read More

Thank you very much for following this up with the customer. I am sorry that the change in the blocking buffer did not improve the results.

I would still like to recommend to the customer to reduce the samples for the Western blot either with...

Read More

1-10 of 12 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"