Anti-Bmi1 抗体 (ab115251)
Key features and details
- Goat polyclonal to Bmi1
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Bmi1 antibody
Bmi1 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to Bmi1 -
由来種
Goat -
アプリケーション
適用あり: IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Horse, Chicken, Cow, Cat, Dog, Pig, Chimpanzee, Orangutan -
免疫原
Synthetic peptide:
DSGSDKANSPAGG
, corresponding to amino acids 252-264 of Human Bmi1 -
ポジティブ・コントロール
- Recombinant Human Bmi1 protein (ab114169) can be used as a positive control in WB. Human Skeletal Muscle tissue
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab115251の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P | (1) |
Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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特記事項 |
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IHC-P
Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2. -
配列類似性
Contains 1 RING-type zinc finger. -
翻訳後修飾
Monoubiquitinated (By similarity). May be polyubiquitinated; which does not lead to proteasomal degradation. -
細胞内局在
Nucleus. Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 554342 Cat
- Entrez Gene: 493647 Chicken
- Entrez Gene: 510666 Cow
- Entrez Gene: 100532731 Human
- Entrez Gene: 648 Human
- Entrez Gene: 12151 Mouse
- Entrez Gene: 100173052 Orangutan
- Entrez Gene: 307151 Rat
see all -
別名
- B lymphoma Mo MLV insertion region (mouse) antibody
- B lymphoma Mo MLV insertion region 1 homolog antibody
- Bmi 1 antibody
see all
画像
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Human Skeletal Muscle (formalin-fixed, paraffin-embedded) stained with ab115251 at 2.5 µg/ml followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
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ab115251 staining Bmi1 in Mouse Pancreatic intraepithelial neoplasia tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with paraformaldehyde, blocked with 10% BSA for 60 minutes at 26°C and antigen retrieval was by heat mediation in Tris-based buffer. The sample was incubated with primary antibody (1/200 in 10% BSA in TBST) at 4°C for 16 hours. A HRP-conjugated Donkey anti-goatpolyclonal (1/1000) was used as the secondary antibody.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab115251 は 3 報の論文で使用されています。
- Tanimura Y et al. Long-term model of colitis-associated colorectal cancer suggests tumor spread mechanism and nature of cancer stem cells. Oncol Lett 21:7 (2021). PubMed: 33240413
- Yoshioka T et al. Bmi1 marks gastric stem cells located in the isthmus in mice. J Pathol 248:179-190 (2019). PubMed: 30689202
- Qiu M et al. MicroRNA-200c suppresses cell growth and metastasis by targeting Bmi-1 and E2F3 in renal cancer cells. Exp Ther Med 13:1329-1336 (2017). WB ; Human . PubMed: 28413473