製品の概要

  • 製品名Anti-Bmf antibody
    Bmf 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Bmf
  • アプリケーション適用あり: WB, ICC/IF, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide:

    EPSQCVEELEDDV

    , corresponding to amino acids 2-14 of Human Bmf. (Peptide available as ab9656).

  • ポジティブ・コントロール
    • HepG2 or 293 cell lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab9655 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 2 µg/ml. Detects a band of approximately 25 kDa.Can be blocked with Human Bmf peptide (ab9656).
ICC/IF Use a concentration of 10 µg/ml.
IHC-P Use at an assay dependent concentration. PubMed: 14668207Note: this was tested against sections embedded in polyester wax.

ターゲット情報

Anti-Bmf antibody 画像

  • Western blot analysis of Bmf expression in human HepG2 (lane 1) and 293 (lane 2) cell lysates with anti-Bmf at 2 µg /ml.
  • ab9655 at 10µg/ml staining Bmf in human Hela cells by Immunocytochemistry.

  • Anti-Bmf antibody (ab9655) at 2 µg/ml + Recombinant Human Bmf protein (ab85485) at 0.1 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 8 minutes

Anti-Bmf antibody (ab9655) 使用論文

This product has been referenced in:
  • Blatt NB  et al. Bz-423 superoxide signals B cell apoptosis via Mcl-1, Bak, and Bax. Biochem Pharmacol 78:966-73 (2009). WB ; Human . Read more (PubMed: 19481066) »
  • Show MD  et al. Phosphorylation of mitogen-activated protein kinase 8 (MAPK8) is associated with germ cell apoptosis and redistribution of the Bcl2-modifying factor (BMF). J Androl 29:338-44 (2008). Read more (PubMed: 18222916) »

See all 3 Publications for this product

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We have actually have 2 antibodies that might be suitable, they both work in mouse and rat:

ab9655 Anti-Bmf antibody
Reacts against mouse, rat, human
92% homology to mouse and monkey.
www.a...

Read More
Application Western blot
Sample Human Cell lysate - other (HELA)
Loading amount 20 µg
Specification HELA
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C
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投稿 Jan 13 2011

Application Western blot
Sample Mouse Cell lysate - other (MEFs)
Loading amount 50 µg
Specification MEFs
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C
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投稿 Jan 13 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cultured Cells (HELA)
Specification HELA
Fixative Paraformaldehyde
Permeabilization Yes - Triton-X-0.5%
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C
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投稿 Nov 22 2010

Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (U87MG tumour cell line)
Loading amount 50 µg
Specification U87MG tumour cell line
Treatment cells treated with LY294002 for 6h
Gel Running Conditions Reduced Denaturing (10% tris glycine)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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投稿 Sep 13 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"