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Products:Cardiovascular >> Lipids / Lipoproteins >> Lipid Metabolism >> Cholesterol Metabolism
Overview
Product name
Anti-BMAL1 antibody - ChIP Grade
BMAL1 抗体 (8件) 一覧
Description
Rabbit polyclonal to BMAL1 - ChIP Grade
Specificity
Detects BMAL 1 / aryl hydrocarbon nuclear translocator 3 (ARNT 3) from hamster tissues as well as recombinant human BMAL 1.
Tested applications
ICC/IF, WB, IP, ChIPmore details
Cross reactivity
Reacts with
Mouse, Rat, Hamster, Human
Immunogen
Synthetic peptide: C-DMIDNDQGSSSPS, corresponding to amino acids 582-594 of Mouse BMAL1(Peptide available as ab4959.)
C-DMIDNDQG SSSPS
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: PBS, 1mg/ml BSA
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Cardiovascular >> Heart >> Cardiac metabolism
Cardiovascular >> Blood >> Blood Pressure regulation
Epigenetics and Nuclear Signaling >> Transcription >> Transcription Factors
Neuroscience >> Neurology process >> Metabolism
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> HLH
Cardiovascular >> Lipids / Lipoproteins >> Lipid Metabolism >> Cholesterol Metabolism
Applications
Show applications key
Our Abpromise guarantee covers the use of ab3350 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use a concentration of 1 µg/ml
WB: 1/200Predicted molecular weight: 69.4 kDa.(This antibody detects a protein which corresponds to the product of a hamster BMAL 1 fusion construct overexpressed in E. coli (110 kDa band)as well as recombinant human BMAL1. )
IP: Use at an assay dependent dilution.
ChIP: Use at an assay dependent dilution.
Target
Function
ARNTL-CLOCK heterodimers activate E-box element (3'-CACGTG-5') transcription of a number of proteins of the circadian clock. This transcription is inhibited in a feedback loop by PER, and also by CRY proteins.
Tissue specificity
Highly expressed in the adult brain, skeletal muscle and heart.
Sequence similarities
Contains 1 basic helix-loop-helix (bHLH) domain.
Contains 1 PAC (PAS-associated C-terminal) domain.
Contains 2 PAS (PER-ARNT-SIM) domains.
Post-translational
modifications
Acetylated on Lys-538 upon dimerization with CLOCK. Acetylation facilitates CRY1-mediated repression.
Phosphorylated upon dimerization with CLOCK.
Sumoylated on Lys-259 upon dimerization with CLOCK.
Cellular localization
Nucleus.
Target information above from: UniProt accessionO00327
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- ARNT like protein 1 brain and muscle antibody
- ARNTL antibody
- Aryl hydrocarbon receptor nuclear translocator like antibody
see all
Database links
- Entrez Gene: 406 Human
- Entrez Gene: 11865 Mouse
- Entrez Gene: 29657 Rat
- Omim: 602550 Human
- SwissProt: O00327 Human
- SwissProt: Q9WTL8 Mouse
- SwissProt: Q9EPW1 Rat
- Unigene: 65734 Human
see all
Anti-BMAL1 antibody - ChIP Grade images:
Western blot - BMAL1 antibody - ChIP Grade (ab3350)
Anti-BMAL1 antibody - ChIP Grade (ab3350) at 1 µg/ml + HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Predicted band size : 69.4 kDa
Observed band size : 75 kDa (why is the actual band size different from the predicted?)
Additional bands at : 51 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 3 minutes
The 75 kDa band observed is comparable to the molecular weight seen with other commercially available antibodies to BMAL1.
Immunocytochemistry/ Immunofluorescence - Anti-BMAL1 antibody - ChIP Grade (ab3350)
ICC/IF image of ab3350 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3350, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-BMAL1 antibody - ChIP Grade (ab3350)
This product has been referenced in:
- Scoma HDet al. The De-Ubiquitinylating Enzyme, USP2, Is Associated with the Circadian Clockwork and Regulates Its Sensitivity to Light. PLoS One 6:e25382 (2011). WB; Mouse.Read more (PubMed: 21966515) »
- Mongrain Vet al. Sleep Loss Reduces the DNA-Binding of BMAL1, CLOCK, and NPAS2 to Specific Clock Genes in the Mouse Cerebral Cortex. PLoS One 6:e26622 (2011). ChIP; Mouse.Read more (PubMed: 22039518) »
See all 7 publications for this product
Publishing research using ab3350? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"