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Products:Cardiovascular >> Lipids / Lipoproteins >> Lipid Metabolism >> Cholesterol Metabolism
Overview
Product name
Anti-BMAL1 antibody
BMAL1 抗体 (8件) 一覧
Description
Mouse polyclonal to BMAL1
Tested applications
Cross reactivity
Reacts with
Fruit fly (Drosophila melanogaster)
Immunogen
Fusion protein: LIDAKTMLPVKTDVPQSLCRLCPGARRSFFCRMKLRTASN NQIKEESDTSSSSRSSTKRKSRLSTGHKYRVIQCTGYLKS WTPIKDEDQDADSDEQTTNL, corresponding to amino acids 179/278 of Drosophila melanogaster BMAL1
LIDAKTMLPV KTDVPQSLCR LCPGARRSFF CRMKLRTASN NQIKEESDTS SSSRSSTKRK SRLSTGHKYR VIQCTGYLKS WTPIKDEDQD ADSDEQTTNL
General notes
Produced from outbred CD1 mice
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Constituents: 50% Glycerol
Purity
Whole antiserum
Primary antibody notes
This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al.PubMed: 1545867; Chambers and Johnston PubMed: 12910245; Barry and Johnston PubMed: 9234514). The animal`s cells produce the protein, which stimulates the animal`s immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cardiovascular >> Heart >> Cardiac metabolism
Cardiovascular >> Blood >> Blood Pressure regulation
Epigenetics and Nuclear Signaling >> Transcription >> Transcription Factors
Neuroscience >> Neurology process >> Metabolism
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> HLH
Cardiovascular >> Lipids / Lipoproteins >> Lipid Metabolism >> Cholesterol Metabolism
Applications
Show applications key
Our Abpromise guarantee covers the use of ab21926 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
Application notes
WB: 1/1000. Predicted molecular weight: 47 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.
Target
Function
ARNTL-CLOCK heterodimers activate E-box element (3'-CACGTG-5') transcription of a number of proteins of the circadian clock. This transcription is inhibited in a feedback loop by PER, and also by CRY proteins.
Tissue specificity
Highly expressed in the adult brain, skeletal muscle and heart.
Sequence similarities
Contains 1 basic helix-loop-helix (bHLH) domain.
Contains 1 PAC (PAS-associated C-terminal) domain.
Contains 2 PAS (PER-ARNT-SIM) domains.
Post-translational
modifications
Acetylated on Lys-538 upon dimerization with CLOCK. Acetylation facilitates CRY1-mediated repression.
Phosphorylated upon dimerization with CLOCK.
Sumoylated on Lys-259 upon dimerization with CLOCK.
Cellular localization
Nucleus.
Target information above from: UniProt accessionO00327
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- ARNT like protein 1 brain and muscle antibody
- ARNTL antibody
- Aryl hydrocarbon receptor nuclear translocator like antibody
see all
References for Anti-BMAL1 antibody (ab21926)
ab21926 has not yet been referenced specifically in any publications.
Publishing research using ab21926? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"