![Western blot - BMAL1 antibody [621C3a] (ab53215) image](#ab53215.gif)
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Products:Cardiovascular >> Lipids / Lipoproteins >> Lipid Metabolism >> Cholesterol Metabolism
Overview
Product name
Anti-BMAL1 antibody [621C3a]
BMAL1 抗体 (8件) 一覧
Description
Mouse monoclonal [621C3a] to BMAL1
Tested applications
WB, Dot Blotmore details
Cross reactivity
Reacts with
Human
Immunogen
Recombinant fragment (Human)
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Protein G purified
Purification notes
ab53215 was purified from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum. Filtered through a 0.22 µm membrane.
Clonality
Monoclonal
Clone number
621C3a
Isotype
IgG1
Research areas
Cardiovascular >> Heart >> Cardiac metabolism
Cardiovascular >> Blood >> Blood Pressure regulation
Epigenetics and Nuclear Signaling >> Transcription >> Transcription Factors
Neuroscience >> Neurology process >> Metabolism
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> HLH
Cardiovascular >> Lipids / Lipoproteins >> Lipid Metabolism >> Cholesterol Metabolism
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Western blot - BMAL1 antibody [621C3a] (ab53215)
![Western blot - BMAL1 antibody [621C3a] (ab53215) image](/ps/datasheet/Images/53/ab53215/ab53215.gif)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab53215 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at an assay dependent dilution. Predicted molecular weight: 68 kDa.
Dot: Use at an assay dependent dilution.
Target
Function
ARNTL-CLOCK heterodimers activate E-box element (3'-CACGTG-5') transcription of a number of proteins of the circadian clock. This transcription is inhibited in a feedback loop by PER, and also by CRY proteins.
Tissue specificity
Highly expressed in the adult brain, skeletal muscle and heart.
Sequence similarities
Contains 1 basic helix-loop-helix (bHLH) domain.
Contains 1 PAC (PAS-associated C-terminal) domain.
Contains 2 PAS (PER-ARNT-SIM) domains.
Post-translational
modifications
Acetylated on Lys-538 upon dimerization with CLOCK. Acetylation facilitates CRY1-mediated repression.
Phosphorylated upon dimerization with CLOCK.
Sumoylated on Lys-259 upon dimerization with CLOCK.
Cellular localization
Nucleus.
Target information above from: UniProt accessionO00327
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- ARNT like protein 1 brain and muscle antibody
- ARNTL antibody
- Aryl hydrocarbon receptor nuclear translocator like antibody
see all
Database links
- Entrez Gene: 406 Human
- Omim: 602550 Human
- SwissProt: O00327 Human
- Unigene: 65734 Human
Anti-BMAL1 antibody [621C3a] images:
Western blot - BMAL1 antibody [621C3a] (ab53215)
Anti-BMAL1 antibody [621C3a] (ab53215) + Immunised recombinant protein
Predicted band size : 68 kDa
References for Anti-BMAL1 antibody [621C3a] (ab53215)
ab53215 has not yet been referenced specifically in any publications.
Publishing research using ab53215? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"