Anti-Blood Group A Antigen 抗体 [HE-195] (ab2522)

製品の概要

  • 製品名
    Anti-Blood Group A Antigen antibody [HE-195]
    Blood Group A Antigen 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [HE-195] to Blood Group A Antigen
  • 特異性
    This antibody recognizes human blood group A antigens, including weak variants A3, AX, A3B, AXB
  • アプリケーション
    適用あり: Agglutinationmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Mixture of erythrocytes of blood group A1 and glycoprotein fraction isolated from the saliva of secretors with blood group A.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: None
    Constituents: Tissue Culture Supernatant
  • 精製度
    Tissue culture supernatant
  • 特記事項(精製)
    Concentrated by ultrafiltration (100 kDa cut-off). Actual immunoglobulin concentration not determined.
  • ポリ/モノ
    モノクローナル
  • クローン名
    HE-195
  • ミエローマ
    unknown
  • アイソタイプ
    IgM
  • 軽鎖の種類
    unknown
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab2522 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Agglutination
  • 追加情報
    In agglutination tests, the minimum titre is 1:256 with erythrocytes of blood group A1
  • ターゲット情報

    • 関連性
      Blood-group antigens are generally defined as molecules formed by sequential addition of saccharides to the carbohydrate side chains of lipids and proteins detected on erythrocytes and certain epithelial cells. The A, B and H antigens are reported to undergo modulation during malignant cellular transformation. Blood group related antigens represent a group of carbohydrate determinants carried on both glycolipids and glycoproteins. They are usually mucin-type, and are detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals. Sixteen genetically and biosynthetically distinct but inter-related specificities belong to this group of antigens, including A, B, H, Lewis A, Lewis B, Lewis X, Lewis Y, and precursor type 1 chain antigens.
    • 細胞内局在
      Cell Membrane
    • 参照データベース

    参考文献

    This product has been referenced in:
    • Nemec M  et al. Murine monoclonal antibodies to human A erythrocytes: differential reactivity with N-acetyl-D-galactosamine. Vox Sang 52:125-8 (1987). Read more (PubMed: 2440181) »

    See 1 Publication for this product

    レビューと Q&A

    I enquired again with the originator of ab2522 and ab2524 and the only information that they were able to give me is that the tissue culture medium is 10% Fetal calf serum at the beginning of cultivation.

    Thank you for your enquiry. Regarding ab2522 and ab2524, no other additives were added to the tissue culture supernatant except azide for preservation. Antibody concentration has not been determined, only specifity by agglutination was performed to con...

    Read More

    Hybridoma is grown in classical RPMI media supplemented with 10%FCS. Supernatant is concentrated about 4 fold by ultrafiltration (100kDa). Specifity is verified by agglutination method. The content of IgM is not determined.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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