Welcome to the Abcam blog

miRNA ポータル

アブカムの FirePlex® マルチプレックス miRNA アッセイの特長

  • 貴重なサンプルを無駄にしません

    血清・血漿、尿などの体液サンプルをたった 20µL から測定できます。体液サンプルからの total RNA 精製は不要です。Circulating マイクロ RNA をダイレクトに測定できます。

  • 幅広いサンプルに対応

    血清や血漿サンプルの場合、採血方法を問わず測定できます。EDTAカリウム、ヘパリンナトリウム、ヘパリンリチウム、クエン酸ナトリウムを用いた採血されたサンプルでそれぞれテスト済みです(詳細はテクニカルノートをご覧ください)。

  • 簡単な操作でマルチプレックス測定

    FirePlex® 独自のパーティクル技術により、最大 68 種類のマイクロ RNA を1 ウェルで同時に測定できます。煩わしい繰り返し操作は不要です。

  • 測定項目の組み合わせは自由

    8 種類の便利なパネル製品をご用意。miRBase 21 に登録されているマイクロ RNA であれば最大 68 種類まで自由に組み合わせ可能です。完全合成マイクロ RNA にも対応できます。

  • 追加のコストは不要

    一般的なフローサイトメーター*で測定でき、専用機器は不要です。解析用専用ソフトウェアは無償でダウンロードできます。
    *受託測定サービスも承っています。
  • *Guava easyCyte Accuri C6, FACSCanto, LSRII and LSRFortessa 等でご使用いただけます。その他の機種についてはお問合せください。
FirePlex® マルチプレックス miRNA アッセイに関するお問い合わせはこちら

図:FirePlex® ワークフロー

ピックアップ情報

MicroRNA profiling direct from biofluids, using Firefly® particle technology Article
FirePlex® は、血清、尿、唾液などの biofulid サンプルから total RNA の精製無しで miRNA を測定できます(英文)
How does the FirePlex® miRNA assay work?  Article
FirePlex® マルチプレックス miRNA アッセイの各ステップの解説です(英文)
主なマイクロ RNA 検出アッセイ法 Article
FirePlex® を、RT-qPCR などの既存テクノロジーと比較しました
Benchmarking the FirePlexᵀᴹ miRNA assays Article
FirePlex® と既存法を比較したテクニカル・ノートがダウンロードできます(英文)
マイクロ RNA のバイオマーカーとしての応用 Article
miRNA のバイオマーカーとしての可能性と最近の知見をご紹介します
MicroRNAs in exosomes Article
エクソソームと miRNA に関する最近の知見をご紹介します
マルチプレックス miRNA サンプル・プロファイリング・サービス Article
サンプルをお預かりしての受託測定サービスも行なっています
感染症関連がんと miRNA Webinar
マルチプレックス・アッセイを用いた miRNA プロファイリングについて解説した Webinar です(英語)
MicroRNA database and target prediction tool index Article
miRNA 関連データベースとターゲット予測ツールの紹介です(英文)
FirePlex® Analysis Workbench: software for FirePlex miRNA assays Webinar
FirePlex® 解析用ソフトウェア(フリーウェア)を解説した Webinar です(英語)

分野別パネル製品

疾患関連パネル
精神・神経疾患関連 Neurology V2 循環器疾患関連 Cardiology V2 がん関連 Oncology V2 免疫疾患関連 Immunology V2
毒性関連パネル
肝毒性用 Liver Tox V2 腎毒性用 Kidney Tox V2 ラット膵臓毒性用
Pancreatic Toxicity
ラット心毒性用
Cardiac Toxicity

使用文献・参考文献

FirePlex® が使用された文献
​Adams BD et al. (2015). miR-34a Silences c-SRC to Attenuate Tumor Growth in Triple Negative Breast Cancer.​ Cancer Res. 2321:2015.
Flowers E, Gadgil M, Auoizerat BE, Kanaya A (2015). Circulating microRNAs associated with glycemic impairment and progression in Asian Indians. Biomarker Research, 3:22.
Flowers E et al. (2015). Circulating microRNA-320a and microRNA-486 predict thiazolidinedione response: Moving towards precision health for diabetes prevention. Metabolism S20026-0495(15)00156-0. 
Glineur SF, De Ron P, Hanon E, Valentin JP, Dremier ​S, da Costa AN.​ ​Paving the route to plasma miR-208a-3p as an acute cardiac injury biomarker: preclinical rat data supports its use in drug safety assessment (2015). Toxicological Sciences, kfv222.
Hoss AG et al. (2015). miR-10b-5p expression in Huntington's disease brain relates to age of onset and the extent of striatal involvement. BMC Med. Genomics 8.1, 10.​
Joerger M et al. (2014). ​​Circulating microRNA profiling in patients with advanced non-squamous NSCLC receiving bevacizumab/erlotinib followed by platinum-based chemotherapy at progression (SAKK 19/05).​
Khodakov D, Wang C, Zhang DY (2016). Diagnostics based on nucleic acid sequence variant profiling: PCR, hybridization, and NGS approaches.​ Adv Drug Deliv Rev. S0169-409X(16)30104-1.
Minh TN. Le Hamar P, Guo C, Basar E, Perdigão-Henriques R, Balaj L, Lieberman J (2014). miR-200 in extracellular vesicles promotes metastasis of breast cancer cells. JCI 124(12), 5109-5128.​
Momen-Heravi F et al. (2015). Increased number of circulating exosomes and their microRNA cargos are potential novel biomarkers in alcoholic hepatitis.​ J Transl Med. ​12;13:261.
Powell J et al. (2016). A cytometry microparticle platform approach for screening tobacco microRNA changes after agrobacterium delivery. Journal of Microbial Methods 127: 230-235.
Ren V, Ambros VR (2015). Caenorhabditis elegans microRNAs of the let-7 family act in innate immune response circuits and confer robust developmental timing against pathogen stress.​ Proc Natl Acad Sci USA 5;112(18).
Ren V et al. (2016). Staufen Negatively Modulates microRNA Activity in Caenorhabditis elegans.​ G3 (Bethesda) ​3;6(5):1227-37.
Sandhu SK, Fassan M, Volinia S, Lovat F, Balatti V, Pekarsky Y, Croce CM (2013) B-cell malignancies in microRNA Eμ-miR-17~92 transgenic mice, PNAS 5, 110(45), 18208.​
Shiu PK, Zhuang JJ, Hunter CP (2014). Assays for direct and indirect effects of C. elegans Endo-siRNAs. Methods Mol. Biol. 1173, 71-87.​
Telonis AG et al. (2015). Dissecting tRNA-derived fragment complexities using personalized transcriptomes reveals novel fragment classes and unexpected dependencies. Oncotarget 22;6(28): 24797-822.
​Zhang Q et al. (2014). Molecular analyses of 6 different types of uterine smooth muscle tumors: Emphasis in atypical leiomyoma. Cancer​ 15;120(20):3165-77.
Zinovyeva AY, Bouasker S, Simard MJ, Hammell CM, Ambros V (2014). Mutations in conserved residues of the C. elegans microRNA Argonaute ALG-1 identify separable functions in ALG-1 miRISC loading and target repression. PLoS Genet. 23, 10(4): e1004286.​
Zou L et al. (2016). Splenic RNA and MicroRNA Mimics Promote Complement Factor B Production and Alternative Pathway Activation via Innate Immune Signaling. J Immunol. ​15;196(6):2788-98.
FirePlex® テクノロジーに関する文献
Appleyard D, Chapin SC, Srinivas R  and Doyle PS (2011). Barcoded hydrogel microparticles for protein detection: synthesis, assay and scanning. Nature Protocols 6, 1761.
Chapin SC and Doyle PS (2011) Ultrasensitive multiplexed microRNA quant on encoded gel microparticles using rolling circle amplification. Analytical Chemistry 83, 7179.
Chapin SC, Pregibon DC and Doyle PS (2009). High-throughput flow alignment of barcoded hydrogel microparticles. Lab on a Chip 9, 3100.​
Chapin SC, Pregibon DC and Doyle PS (2011). Rapid microRNA profiling on encoded gel microparticles. Agnew Chem Int Ed 50, 2289.
Dendukuri D, Pregibon DC, Collins J, Hatton TA and Doyle PS (2006). Continuous-flow lithography for high-throughput microparticle synthesis. Nature Materials 5, ​369.​​
Pregibon DC and Doyle PS (2009). Optimization of encoded hydrogel particles for nucleic acid quantification. Analytical Chemistry 81, 4873.
Pregibon DC, Toner M and Doyle PS (2007). Multifunctional encoded particles for high-throughput biomolecule analysis. Science 315, 1393.​

Cytokeratin ~ 注目ターゲットのおすすめ製品


ウサギ・モノクローナル 抗体

ターゲット名

交差種

適用・アプリケーション

文献

Abreview
数 評価

製品番号

Cytokeratin 1Hu, MS, Rat WB, IHC-P, IHC-Fr ab185628
Cytokeratin 4HuFlow Cyt, ICC, ICC/IF, IHC-P, IP, WB6 ab51599
Cytokeratin 5Hu, MsIHC-Fr, ICC/IF, Flow Cyt, IHC-P, WB8 ab214586
Cytokeratin 6 HuICC, IHC-P, WB1 ab52620
Cytokeratin 7 Hu, Ms, RatICC/IF, IHC-Fr, IHC-P, WB39ab181598
Cytokeratin 7
BSA and Azide free
Hu, Ms, Rat ICC/IF, WB, IHC-Fr, IHC-P2 ab220804
Cytokeratin 8
BSA and Azide free
Hu, MsWB, IP, IHC-P, Flow Cyt, ICC/IF14 ab217173
Cytokeratin 8+18 Ms, HuFlow Cyt, ICC/IF, IHC-P, IP, WB173 ab53280
Cytokeratin 8 (pS73)Hu, Ms, RatICC/IF, IHC-Fr, IHC-P, WB56 ab32579
Cytokeratin 10Hu, Ms, RatICC/IF, IHC-Fr, IHC-P, WB87 ab76318
Cytokeratin 10
BSA and Azide free
Hu, Ms, Rat ICC/IF, IHC-Fr, WB, IHC-P8ab220806
Cytokeratin 13 Ms, HuICC, IHC-Fr, IHC-P, WB71 ab92551
Cytokeratin 14 Hu, Ms, RatICC/IF, IHC-P, WB23 ab181595
Cytokeratin 14
Alexa Fluor® 488
HuFlow Cyt, ICC/IF2 ab192055
Cytokeratin 15Hu, MsFlow Cyt, ICC/IF, IHC-P, WB81 ab52816
Cytokeratin 16 HuFlow Cyt, ICC, IHC-P, WB91 ab76416
Cytokeratin 17Hu, Ms, RatFlow Cyt, ICC/IF, IHC-P, WB3 ab109725
Cytokeratin 18 HuFlow Cyt, ICC/IF, IHC-Fr, IHC-P, WB145 ab32118
Cytokeratin 19 Hu, MsFlow Cyt, ICC/IF, IHC-P, WB237 ab52625
Cytokeratin 19
BSA and Azide free
Hu, Ms ICC/IF, Flow Cyt, IHC-P, WB8ab195872
Cytokeratin 20Goat, Hu, Mmst, Pig, RatFlow Cyt, ICC, IHC-Fr, IHC-P, IP, WB913 ab76126
Cytokeratin 20
BSA and Azide free
Goat, Hu, Mmst, Pig, Rat Flow Cyt, ICC/IF, IP, IHC-Fr, WB, IHC-P7ab219589

抗体

ターゲット名

交差種

適用・アプリケーション

文献

Abreview
数 評価

製品番号

Acidic CytokeratinMs-M Hu, Ms, Cow, Dog, Rb, Rat, Chk, Turt, MkFlow Cyt, ICC/IF, IHC-Fr, IHC-P, WB ab187381
Cytokeratin 1Ms-MMs, Rat, Chk, Cow, Mk, Rb, TurtIHC-Fr, IHC-P, WB92 ab9286
Cytokeratin 2Ms-M Ms, Rat, Rb, Chk, Cow, Hu, Mk, PigEM, IHC-Fr, IHC-P, WB ab16111
Cytokeratin 3Ms-MHu, Cow, RbFlow Cyt, ICC/IF, IHC-P, WB51 ab77869
Cytokeratin 3+12Ms-MHu, Ms, Cow, RbICC/IF, IHC-Fr, IHC-P, WB31 ab68260
Cytokeratin 4Ms-MHu, Cat, Dog, ZfshFlow Cyt, ICC, WB6 ab9004
Cytokeratin 5Rb-PHu, Ms, Rat, PigELISA, Flow Cyt, ICC/IF, IHC-FoFr, IHC-Fr, IHC-P, WB174 ab53121
Cytokeratin 5 + 6Ms-MHuIHC-P41 ab17133
Cytokeratin 5 + 8Ms-MHu, Ms, Rat, Dog, Hm, Pig, RbDot, Flow Cyt, ICC/IF, IHC-Fr, IHC-P, WB5 ab9005
Cytokeratin 6Ms-MHu, MsFlow Cyt, ICC, IHC-Fr, IHC-P, WB3 ab18586
Cytokeratin 7Ms-MHu, Ms, Rat, Cat, Dog, Goat, Hm, Pig, ZfshFlow Cyt, ICC, ICC/IF, IHC-Fr, IHC-P, WB2312 ab9021
Cytokeratin 8Ms-MHu, Rat, RbFlow Cyt, ICC, ICC/IF, IHC-Fr, IHC-P, WB139 ab9023
Cytokeratin 8+18Ms-MHuFlow Cyt, ICC/IF, IHC-P, WB11 ab17139
Cytokeratin 10Ms-MHu, Ms, Cat, Dog, ShpFlow Cyt, ICC, IHC-Fr, IHC-P, WB133 ab9026
Cytokeratin 10 + 13Ms-M Cat, Hu, Zfsh, DogFlow Cyt, ICC, IHC-Fr, IHC-P, WB12 ab9383
Cytokeratin 13Ms-MHu, Ms, RbICC/IF, IHC-P, WB102 ab16112
Cytokeratin 15Ms-MHu, Ms, Rat, CowFlow Cyt, ICC/IF, IHC-P22 ab80522
Cytokeratin 17Rb-PHu, Ms, RatELISA, ICC, ICC/IF, IHC-P, WB81 ab53707
Cytokeratin 18Ms-M Ms, Rat, Shp, Goat, Hrs, Hm, Cow, Dog, Hu, Pig, Mmst, CatFlow Cyt, ICC, ICC/IF, IHC-Fr, IHC-P, IP, WB4219 ab668
Cytokeratin 19Ms-MHuELISA, Flow Cyt, ICC, ICC/IF, IHC-Fr, IHC-P, IP, WB167 ab7754

タンパク質・ペプチド

製品名

由来

適用・アプリケーション

製品番号

Cytokeratin 1 protein HuELISA, SDS-PAGE, WBab114282
Cytokeratin 7 proteinHuELISA, SDS-PAGE, WBab132933
Cytokeratin 8 proteinHuSDS-PAGEab156970
Cytokeratin 10 protein HuELISA, SDS-PAGE, WBab114223
Cytokeratin 13 proteinHuELISA, SDS-PAGE, WBab112315
Cytokeratin 16 protein HuELISA, SDS-PAGE, WBab114405
Cytokeratin 17 proteinHuELISA, SDS-PAGE, WBab116974
Cytokeratin 18 proteinHuSDS-PAGE, WBab73638
タンパク質・ペプチド一覧

キット

製品名

交差種

容量

製品番号

Cytokeratin 14 Matched 抗体 Pair KitHu2 x 96 tests
10 x 96 tests
ab221423
キット一覧

ライゼート

製品名

適用・アプリケーション

製品番号

Cytokeratin 4 293T Transfected Lysate - (positive control)WBab94137
Cytokeratin 17 293T Transfected Lysate - (positive control)WBab94233
Cytokeratin 19 293T Transfected Lysate - (positive control)WBab94060

Cytokeratin について

Cytokeratin1
Function
機能
May regulate the activity of kinases such as PKC and SRC via binding to integrin beta-1 (ITB1) and the receptor of activated protein kinase C (RACK1/GNB2L1).
Tissue specificity
組織特異性
The source of this protein is neonatal foreskin. The 67-kDa type II keratins are expressed in terminally differentiating epidermis.
Involvement in disease
関連疾患
  • bullous congenital ichthyosiform erythroderma
  • ichthyosis hystrix Curth-Macklin type
  • palmoplantar keratoderma non-epidermolytic
  • ichthyosis annular epidermolytic
  • palmoplantar keratoderma striate type 3
Cellular localization
細胞内局在性
Cell membrane. Located on plasma membrane of neuroblastoma NMB7 cells.

Cytokeratin2
Function
機能
Probably contributes to terminal cornification.

Cytokeratin3
Tissue specificity
組織特異性
Cornea specific.
Involvement in disease
関連疾患
Meesmann corneal dystrophy

Cytokeratin4
Tissue specificity
組織特異性
Detected in the suprabasal layer of the stratified epithelium of the esophagus, exocervix, vagina, mouth and lingual mucosa, and in cells and cell clusters in the mucosa and serous gland ducts of the esophageal submucosa (at protein level). Expressed widely in the exocervix and esophageal epithelium, with lowest levels detected in the basal cell layer.
Involvement in disease
関連疾患
white sponge nevus of cannon

Cytokeratin5
Involvement in disease
関連疾患
  • epidermolysis bullosa simplex Dowling-Meara type
  • epidermolysis bullosa simplex with migratory circinate erythema
  • epidermolysis bullosa simplex Weber-Cockayne type
  • epidermolysis bullosa simplex Koebner type
  • epidermolysis bullosa simplex with mottled pigmentation
  • Dowling-Degos disease

Cytokeratin6
Tissue specificity
組織特異性
Constitutively expressed in distinct types of epithelia such as those in oral mucosa, esophagus, papillae of tongue and hair follicle outer root sheath.

Cytokeratin7
Function
機能
Blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7).
Tissue specificity
組織特異性
Expressed in cultured epidermal, bronchial and mesothelial cells but absent in colon, ectocervix and liver. Observed throughout the glandular cells in the junction between stomach and esophagus but is absent in the esophagus.
Cellular localization
細胞内局在性
Cytoplasm.

Cytokeratin8
Function
機能
Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
Tissue specificity
組織特異性
Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity.
Cellular localization
細胞内局在性
Cytoplasm. Nucleus, nucleoplasm. Nucleus matrix.

Cytokeratin9
Function
機能
May serve an important special function either in the mature palmar and plantar skin tissue or in the morphogenetic program of the formation of these tissues. Plays a role in keratin filament assembly.
Tissue specificity
組織特異性
Expressed in the terminally differentiated epidermis of palms and soles.
Involvement in disease
関連疾患
palmoplantar keratoderma epidermolytic

Cytokeratin10
Tissue specificity
組織特異性
Seen in all suprabasal cell layers including stratum corneum.
Involvement in disease
関連疾患
  • bullous congenital ichthyosiform erythroderma
  • ichthyosis annular epidermolytic

Cytokeratin13
Tissue specificity
組織特異性
Expressed in some epidermal sweat gland ducts (at protein level) and in exocervix, esophagus and placenta.
Involvement in disease
関連疾患
  • bullous congenital ichthyosiform erythroderma
  • ichthyosis annular epidermolytic

Cytokeratin14
Function
機能
The nonhelical tail domain is involved in promoting KRT5-KRT14 filaments to self-organize into large bundles and enhances the mechanical properties involved in resilience of keratin intermediate filaments in vitro.
Tissue specificity
組織特異性
Detected in the basal layer, lowered within the more apically located layers specifically in the stratum spinosum, stratum granulosum but is not detected in stratum corneum. Strongly expressed in the outer root sheath of anagen follicles but not in the germinative matrix, inner root sheath or hair. Found in keratinocytes surrounding the club hair during telogen.
Involvement in disease
関連疾患
  • epidermolysis bullosa simplex Dowling-Meara type
  • epidermolysis bullosa simplex Weber-Cockayne type
  • epidermolysis bullosa simplex Koebner type
  • epidermolysis bullosa simplex autosomal recessive
  • Naegeli-Franceschetti-Jadassohn syndrome
  • dermatopathia pigmentosa reticularis
Cellular localization
細胞内局在性
Cytoplasm. Nucleus. Expressed in both as a filamentous pattern.

Cytokeratin15
Tissue specificity
組織特異性
Expressed in a discontinuous manner in the basal cell layer of adult skin epidermis, but continuously in the basal layer of fetal skin epidermis and nail. Also expressed in the outer root sheath above the hair bulb in hair follicle (at protein level). Expressed homogeneously in all cell layers of the esophagus and exocervix, but detected in the basal cell layer only of oral mucosa, skin and in the basal plus the next two layers of the suprabasal epithelium of the palate.

Cytokeratin16
Tissue specificity
組織特異性
Expressed in the hair follicle, nail bed and in mucosal stratified squamous epithelia and, suprabasally, in oral epithelium and palmoplantar epidermis. Also found in luminal cells of sweat and mammary gland ducts.
Involvement in disease
関連疾患
  • pachyonychia congenita type 1
  • palmoplantar keratoderma non-epidermolytic focal
  • unilateral palmoplantar verrucous nevus

Cytokeratin17
Function
機能
May play a role in the formation and maintenance of various skin appendages, specifically in determining shape and orientation of hair. May be a marker of basal cell differentiation in complex epithelia and therefore indicative of a certain type of epithelial "stem cells". May act as an autoantigen in the immunopathogenesis of psoriasis, with certain peptide regions being a major target for autoreactive T-cells and hence causing their proliferation. Required for the correct growth of hair follicles, in particular for the persistence of the anagen (growth) state. Modulates the function of TNF-alpha in the specific context of hair cycling. Regulates protein synthesis and epithelial cell growth through binding to the adapter protein SFN and by stimulating Akt/mTOR pathway. Involved in tissue repair.
Tissue specificity
組織特異性
Expressed in the outer root sheath and medulla region of hair follicle specifically from eyebrow and beard, digital pulp, nail matrix and nail bed epithelium, mucosal stratified squamous epithelia and in basal cells of oral epithelium, palmoplantar epidermis and sweat and mammary glands. Also expressed in myoepithelium of prostate, basal layer of urinary bladder, cambial cells of sebaceous gland and in exocervix (at protein level).
Involvement in disease
関連疾患
  • pachyonychia congenita type 2
  • steatocystoma multiplex
Cellular localization
細胞内局在性
Cytoplasm.

Cytokeratin18
Function
機能
Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.
Tissue specificity
組織特異性
Expressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma.
Involvement in disease
関連疾患
Defects in KRT18 are a cause of cirrhosis (CIRRH)
Cellular localization
細胞内局在性
Cytoplasm > perinuclear region.

Cytokeratin19
Function
機能
Involved in the organization of myofibers. Together with KRT8, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
Tissue specificity
組織特異性
Expressed in a defined zone of basal keratinocytes in the deep outer root sheath of hair follicles. Also observed in sweat gland and mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, ectocervical epithelium (at protein level). Expressed in epidermal basal cells, in nipple epidermis and a defined region of the hair follicle. Also seen in a subset of vascular wall cells in both the veins and artery of human umbilical cord, and in umbilical cord vascular smooth muscle. Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma in structures that contain dystrophin and spectrin.

Cytokeratin20
Function
機能
Plays a significant role in maintaining keratin filament organization in intestinal epithelia. When phosphorylated, plays a role in the secretion of mucin in the small intestine.
Tissue specificity
組織特異性
Expressed predominantly in the intestinal epithelium. Expressed in luminal cells of colonic mucosa. Also expressed in the Merkel cells of keratinized oral mucosa; specifically at the tips of some rete ridges of the gingival mucosa, in the basal layer of the palatal mucosa and in the taste buds of lingual mucosa.
Cellular localization
細胞内局在性
Cytoplasm.

関連リンク


注目ターゲット

Cancer
ポータル

上皮間葉転換

Ki67 抗体 ~ 臨床への応用

Ki67


Alexa Fluor® is a registered trademark of Life Technologies. Alexa Fluor® dye conjugates contain(s) technology licensed to Abcam by Life Technologies.

Ubiquitin ~ 注目ターゲットのおすすめ製品


ウサギ・モノクローナル 抗体

ターゲット名

交差種

適用・アプリケーション

文献

Abreview
数 評価

製品番号

UbiquitinHu, Ms, RatFlow Cyt, ICC/IF, IHC-P, WB51 ab134953
Ubiquitin (linkage-specific K48) Hu, Ms, Rat ICC/IF, IHC-P, Flow Cyt, WB96 ab140601
Ubiquitin (linkage-specific K63)Hu, Ms, Rat WB, IHC-P, ICC/IF, IHC-FoFr, Flow Cyt43 ab179434
Ubiquitin (linkage-specific K27) Hu, Ms, RatIHC-P, WB, Flow Cyt, ICC/IF ab181537
Ubiquitin (linkage-specific K63) Alexa Fluor® 647 HuFlow Cyt, ICC/IF ab192489
Ubiquitin (linkage-specific K48) Alexa Fluor® 488 HuICC/IF ab204274
Ubiquitin Alexa Fluor® 488 HuICC/IF ab205467
Ubiquitin Alexa Fluor® 647 HuICC/IF ab205468

抗体

ターゲット名

交差種

適用・アプリケーション

文献

Abreview
数 評価

製品番号

UbiquitinRb-PHu, Ms, Rat, AGMkICC/IF, IHC-FoFr, IHC-P, IP, WB39 29ab7780
UbiquitinMs-M Ms, Chk, Cow, Hu, At, Ce, Dm, DCV, RatELISA, ICC/IF, IHC-Fr, IHC-P, WB257ab7254
Ubiquitin (HRP)Ms-M n/aIHC-Fr, IHC-P, WB ab191809
抗体一覧

タンパク質・ペプチド

製品名

由来

適用・アプリケーション

製品番号

Ubiquitin K33 only protein (Recombinant / Active )HuFuncS, MS, Other, SDS-PAGE, WBab157076
Ubiquitin K48 only protein (Recombinant / Active )HuFuncS, MS, Other, SDS-PAGE, WBab157048
Ubiquitin K63 only protein (Recombinant / Active )HuFuncS, MS, Other, SDS-PAGE, WBab157056
Tetra-ubiquitin (linkage-specific K63) protein (biotinylated /Recombinant / Active)HuFuncS, SDS-PAGEab189683
Ubiquitin (mutated K33) protein (Recombinant / Chemical Free)HuConjugation, SDS-PAGEab80742
Poly-ubiquitin (linkage-specific K48) protein (Recombinant / Active )HuFuncS, SDS-PAGEab189706
Tetra-ubiquitin (linkage-specific K11) protein (Recombinant)HuHPLC, WBab180028
Triubiquitin (linkage-specific K48) protein (Recombinant)HuMS, SDS-PAGE, WBab166765
Di-ubiquitin (linkage-specific K11) protein (Recombinant)HuSDS-PAGEab206451
Di-ubiquitin (linkage-specific K48) (mutated K48 R) protein (Recombinant)HuFuncS, SDS-PAGEab207972
タンパク質・ペプチド一覧

キット

製品名

交差種

容量

製品番号

E2-Ubiquitin Conjugation Kit50testab139472
Ubiquitylation Assay Kit20testab139467
Ubiquitylation Assay Kit (HeLa lysate-based)20testab139471
キット一覧

生理活性物質

製品名

解説

製品番号

NSC-632839Nonselective ubiquitin isopeptidase inhibitorab144599
SMER3 Potent and selective ubiquitin C-terminal hydrolase L1 inhibitorab144596
BML-282 Potent and selective ubiquitin C-terminal hydrolase L1 inhibitorab144529
TCIDPotent and selective ubiquitin C-terminal hydrolase-L3 inhibitorab144528
PYR-41 Ubiquitin-activating enzyme (E1) inhibitorab141469
生理活性物質一覧

Ubiquitin について

Relevance
関連性
Function: Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling. Similarity: Belongs to the ubiquitin family. Contains 3 ubiquitin-like domains.
Cellular localization
細胞内局在性
Cell Membrane, Cytoplasmic and Nuclear

関連リンク


注目ターゲット

Ubiquitin chains poster

An Introduction to Post-Translational Modifications Webinar


Alexa Fluor® is a registered trademark of Life Technologies. Alexa Fluor® dye conjugates contain(s) technology licensed to Abcam by Life Technologies.

PI 3 Kinase ~ 注目ターゲットのおすすめ製品


ウサギ・モノクローナル 抗体

ターゲット名

交差種

適用・アプリケーション

文献

Abreview
数 評価

製品番号

PI 3 Kinase catalytic subunit alpha Hu, MsICC/IF, IP, WB51 ab40776
PI 3 Kinase catalytic subunit alpha
Alexa Fluor® 488
HuICC/IF ab202671
PI 3 Kinase p55 gamma Hu, Ms, RatICC/IF, IHC-P, WB1 ab186612
PI 3 Kinase p85 alpha Hu, Ms, Rat WB, ICC/IF, Flow Cyt, IP ab191606
PI 3 Kinase p85 beta Hu, RatFlow Cyt, ICC/IF, IHC-P, IP, WB ab180967
PI 3 Kinase p110 betaHu, Ms, RatFlow Cyt, ICC/IF, IHC-P, WB3 ab151549
PI 3 Kinase p110 beta
Alexa Fluor® 488
HuICC/IF ab202666
PI 3 Kinase p110 delta Hu, Ms, RatICC, WB2 ab109006
PI 3 Kinase p110 delta (HRP) Ms, HuWB ab200773
PI 3 Kinase regulatory subunit 4Hu, Ms, RatFlow Cyt, ICC/IF, IHC-P, WB ab124817

抗体

ターゲット名

交差種

適用・アプリケーション

文献

Abreview
数 評価

製品番号

PI 3 Kinase catalytic subunit alphaMs-MHuELISA, Flow Cyt, ICC/IF, WB ab126819
PI 3 Kinase p85 alpha Ms-M HuELISA, ICC, ICC/IF, IHC-P, WB94 ab86714
PI 3 Kinase p85 alphaMs-M Hu, MsFlow Cyt, ICC/IF, IHC-P, WB2 ab189403
PI 3 Kinase p85 alpha (pY607)Rb-P Ms, Rat, HuWB8 ab182651
PI 3 Kinase p85 betaMs-M Hu, MkIHC-Fr, IP, WB4 ab28356
PI 3 Kinase p110 deltaRb-P Hu, MsELISA, ICC, ICC/IF, IP, WB93 ab1678
抗体一覧

タンパク質・ペプチド

製品名

由来

適用・アプリケーション

製品番号

PI 3 Kinase catalytic subunit gamma protein (Active / Recombinant)HuFuncS, SDS-PAGE, WBab125538
PI 3 Kinase catalytic subunit alpha protein (Recombinant)HuELISA, WBab159127
PI 3 Kinase p55 gamma protein (Recombinant)HuSDS-PAGE, WBab127108
PI 3 Kinase p85 beta protein (Recombinant)HuSDS-PAGE, WBab125568
PI 3 Kinase p110 alpha + PI 3 kinase p85 alpha protein (Active / Recombinant)HuFuncS, SDS-PAGE, WBab91093
タンパク質・ペプチド一覧

キット

製品名

交差種

容量

製品番号

PI3K p85 In-Cell ELISA Kit Total + Phospho-tyrosineMs, Rat, Hu1×96tests
5×96tests
ab207484
PI3K p85 In-Cell ELISA Kit Total + Phospho-tyrosine (Chemiluminescent)Ms, Rat, Hu1×96tests
5×96tests
ab207485
キット一覧

生理活性物質

製品名

解説

製品番号

3-Methyladenine Selective PI3K inhibitorab120841
LY 303511 PI3-kinase inhibitor. Kv channel blocker.ab145193
Viridiol Steroid antibiotic agent. PI 3-kinase inhibitor.ab144384
CAY10626 Potent PI3Kα/mTOR inhibitorab120903
LY 294002 PI3-kinase inhibitorab120243
LY 303511 dihydrochloride Negative control for LY 294002 (ab120243)ab120956
生理活性物質一覧

PI 3 Kinase について

PI 3 Kinase catalytic subunit alpha
Function
機能
Phosphorylates PtdIns, PtdIns4P and PtdIns(4,5)P2 with a preference for PtdIns(4,5)P2.
Involvement in disease
関連疾患
Defects in PIK3CA are associated with colorectal cancer (CRC) [MIM:114500]. Defects in PIK3CA are a cause of susceptibility to breast cancer (BC) [MIM:114480]. A common malignancy originating from breast epithelial tissue. Breast neoplasms can be distinguished by their histologic pattern. Invasive ductal carcinoma is by far the most common type. Breast cancer is etiologically and genetically heterogeneous. Important genetic factors have been indicated by familial occurrence and bilateral involvement. Mutations at more than one locus can be involved in different families or even in the same case. Defects in PIK3CA are a cause of susceptibility to ovarian cancer (OC) [MIM:167000]. Ovarian cancer common malignancy originating from ovarian tissue. Although many histologic types of ovarian neoplasms have been described, epithelial ovarian carcinoma is the most common form. Ovarian cancers are often asymptomatic and the recognized signs and symptoms, even of late-stage disease, are vague. Consequently, most patients are diagnosed with advanced disease. Defects in PIK3CA may underlie hepatocellular carcinoma (HCC) [MIM:114550]. Defects in PIK3CA are a cause of keratosis seborrheic (KERSEB) [MIM:182000]. A common benign skin tumor. Seborrheic keratoses usually begin with the appearance of one or more sharply defined, light brown, flat macules. The lesions may be sparse or numerous. As they initially grow, they develop a velvety to finely verrucous surface, followed by an uneven warty surface with multiple plugged follicles and a dull or lackluster appearance.
Sequence similarities
配列類似性
  • Belongs to the PI3/PI4-kinase family.
  • Contains 1 C2 domain.
  • Contains 1 PI3K/PI4K domain.

PI 3 Kinase p55 gamma
Function
機能
Binds to activated (phosphorylated) protein-tyrosine kinases through its SH2 domain and regulates their kinase activity. During insulin stimulation, it also binds to IRS-1.
Tissue specificity
組織特異性
Highest levels in brain and testis. Lower levels in adipose tissue, kidney, heart, lung and skeletal muscle.
Sequence similarities
配列類似性
  • Belongs to the PI3K p85 subunit family.
  • Contains 2 SH2 domains.

PI 3 Kinase p85 alpha
Function
機能
Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues.
Tissue specificity
組織特異性
Isoform 2 is expressed in skeletal muscle and brain, and at lower levels in kidney and cardiac muscle. Isoform 2 and isoform 4 are present in skeletal muscle (at protein level).
Sequence similarities
配列類似性
  • Belongs to the PI3K p85 subunit family.
  • Contains 1 Rho-GAP domain.
  • Contains 2 SH2 domains.
  • Contains 1 SH3 domain.
Post-translational modifications
翻訳後修飾
Polyubiquitinated in T-cells by CBLB; which does not promote proteasomal degradation but impairs association with CD28 and CD3Z upon T-cell activation. Phosphorylated. Dephosphorylated by PTPRJ.

PI 3 Kinase p85 beta
Function
機能
Regulatory subunit of phosphoinositide-3-kinase (PI3K), a kinase that phosphorylates PtdIns(4,5)P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Binds to activated (phosphorylated) protein-tyrosine kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Indirectly regulates autophagy (PubMed:23604317). Promotes nuclear translocation of XBP1 isoform 2 in a ER stress- and/or insulin-dependent manner during metabolic overloading in the liver and hence plays a role in glucose tolerance improvement.
Involvement in disease
関連疾患
Megalencephaly-polymicrogyria-polydactyly-hydrocephalus syndrome 1
Sequence similarities
配列類似性
  • Belongs to the PI3K p85 subunit family.
  • Contains 1 Rho-GAP domain.
  • Contains 2 SH2 domains.
  • Contains 1 SH3 domain.
Post-translational modifications
翻訳後修飾
Phosphorylated in response to signaling from activated receptor-type protein kinases (PubMed:19690332, PubMed:20068231). Dephosphorylated by PTPRJ (PubMed:18348712). Dephosphorylated at Tyr-655 by PTPN13. Phosphorylation of Tyr-655 impairs while its dephosphorylation promotes interaction with FBXL2 and SCF(FBXL2)-mediated polyubiquitination (PubMed:23604317). Ubiquitinated. Polyubiquitination by the SCF(FBXL2) complex probably promotes proteasomal degradation of PIK3R2.

PI3 Kinase p110 beta
Function
機能
Phosphorylates PtdIns, PtdIns4P and PtdIns(4,5)P2 with a preference for PtdIns(4,5)P2.
Tissue specificity
組織特異性
Expressed ubiquitously.
Sequence similarities
配列類似性
  • Belongs to the PI3/PI4-kinase family.
  • Contains 1 PI3K/PI4K domain.

PI 3 Kinase p110 delta
Tissue specificity
組織特異性
Expressed predominantly in leukocytes.
Sequence similarities
配列類似性
  • Belongs to the PI3/PI4-kinase family.
  • Contains 1 PI3K/PI4K domain.
Post-translational modifications
翻訳後修飾
Autophosphorylation on Ser-1039 results in the almost complete inactivation of the lipid kinase activity.

PI 3 Kinase regulatory subunit 4
Function
機能
Regulatory subunit of the PI3K complex. May regulate membrane trafficking late in the endocytic pathway.
Tissue specificity
組織特異性
Ubiquitously expressed.
Sequence similarities
配列類似性
  • Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
  • Contains 3 HEAT repeats.
  • Contains 1 protein kinase domain.
  • Contains 7 WD repeats.
Post-translational modifications
翻訳後修飾
Myristoylated.Probably autophosphorylated.

関連リンク


注目ターゲット

Cancer ポータル



Alexa Fluor® is a registered trademark of Life Technologies. Alexa Fluor® dye conjugates contain(s) technology licensed to Abcam by Life Technologies.

Rho ~ 注目ターゲットのおすすめ製品


ウサギ・モノクローナル 抗体

ターゲット名

交差種

適用・アプリケーション

文献

Abreview
数 評価

製品番号

RhoHu, Ms, RatICC/IF, IHC-P, WB23 ab40673
Rho
Alexa Fluor® 488
HuICC/IF ab203324
RhoA Hu, Ms, RatFlow Cyt, ICC/IF, WB2 ab187027
Rho A + B + C Hu, Ms, RatFlow Cyt, ICC/IF, IHC-P, WB ab188103
RhoGDIHu, Ms, RatICC, IHC-P, WB11 ab133248
RhoGDI (HRP) Ms, HuIHC-P, WB ab200183

抗体

ターゲット名

交差種

適用・アプリケーション

文献

Abreview
数 評価

製品番号

RhoAMs-MHuELISA, Flow Cyt, ICC/IF, IHC-P, WB118 ab54835
Rho A + B + CMs-M Ms, Rat, HuICC/IF, IP, WB ab175328
RHOCMs-MHu, RatFlow Cyt, WB1 ab54837
RhoGDIMs-MHu, Ms, MkFlow Cyt, ICC/IF, WB2 ab135252
抗体一覧

タンパク質・ペプチド

製品名

由来

適用・アプリケーション

製品番号

Rho GTPase-activating protein 24 protein (Recombinant )HuELISA, WBab164402
RHOG protein (Recombinant )HuMS, SDS-PAGEab128454
RHOB protein (Recombinant )HuMS, SDS-PAGEab107139
RhoGDI protein (Recombinant )HuELISA, SDS-PAGE, WBab132108
RhoA protein (Recombinant )HuSDS-PAGE, WBab101594
タンパク質・ペプチド一覧

キット

製品名

交差種

容量

製品番号

RhoA G17A Agarose Beads (Active Rho-GEF)400ugab211183
RhoA Activation Assay Kit Hu, Ms, Rat5test
20test
ab211164
RhoB Activation Assay Kit Hu, Ms, Rat5test
20test
ab211165
RhoC Activation Assay Kit Hu, Ms, Rat5test
20test
ab211166
キット一覧

生理活性物質

製品名

解説

製品番号

Y-27632 dihydrochloride
NEW
Selective ROCK inhibitor. Suppress cell death during cell dispersion of human ES and iPS cellsab120129
Thiazovivin (DMSO solution)Rho kinase inhibitor. Thiazovivin (ab142081) solution. Reverses Ang II-induced MYPT1 and MLC phosphorylation. Enhances fibroblast reprogramming efficiency during pluripotent stem cell formation.ab146618
N-Boc-Y-27632Protected Y-27632, a Rho kinase (ROCK) inhibitorab143784
HA 1100 hydrochloride (Hydroxyfasudil)Selective, ATP-competitive, reversible Rho-kinase inhibitorab145524
生理活性物質一覧

Rho について

Rho
Function
機能
Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Involved in a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis. Plays an essential role in cleavage furrow formation. Required for the apical junction formation of keratinocyte cell-cell adhesion. Serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague, and Yersinia pseudotuberculosis, which causes gastrointestinal disorders. Stimulates PKN2 kinase activity. May be an activator of PLCE1. Activated by ARHGEF2, which promotes the exchange of GDP for GTP. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity. In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization.
Sequence similarities
配列類似性
  • Belongs to the small GTPase superfamily. Rho family.
Post-translational modifications
翻訳後修飾
Substrate for botulinum ADP-ribosyltransferase. Cleaved by yopT protease when the cell is infected by some Yersinia pathogens. This removes the lipid attachment, and leads to its displacement from plasma membrane and to subsequent cytoskeleton cleavage. AMPylation at Tyr-34 and Thr-37 are mediated by bacterial enzymes in case of infection by H.somnus and V.parahaemolyticus, respectively. AMPylation occurs in the effector region and leads to inactivation of the GTPase activity by preventing the interaction with downstream effectors, thereby inhibiting actin assembly in infected cells. It is unclear whether some human enzyme mediates AMPylation; FICD has such ability in vitro but additional experiments remain to be done to confirm results in vivo. Phosphorylation by PRKG1 at Ser-188 inactivates RHOA signaling. Ubiquitinated by the BCR(BACURD1) and BCR(BACURD2) E3 ubiquitin ligase complexes, leading to its degradation by the proteasome, thereby regulating the actin cytoskeleton and cell migration.
Cellular localization
細胞内局在性
Cell membrane. Cytoplasm > cytoskeleton. Cleavage furrow. Cytoplasm > cell cortex. Midbody. Localized to cell-cell contacts in calcium-treated keratinocytes (By similarity). Translocates to the equatorial region before furrow formation in a ECT2-dependent manner. Localizes to the equatorial cell cortex (at the site of the presumptive furrow) in early anaphase in a activated form and in a myosin- and actin-independent manner.

RhoA
Function
機能
Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague, and Yersinia pseudotuberculosis, which causes gastrointestinal disorders. May be an activator of PLCE1. Activated by ARHGEF2, which promotes the exchange of GDP for GTP.
Sequence similarities
配列類似性
  • Belongs to the small GTPase superfamily. Rho family.
Post-translational modifications
翻訳後修飾
Substrate for botulinum ADP-ribosyltransferase. Cleaved by yopT protease when the cell is infected by some Yersinia pathogens. This removes the lipid attachment, and leads to its displacement from plasma membrane and to subsequent cytoskeleton cleavage. AMPylation at Tyr-34 and Thr-37 are mediated by bacterial enzymes in case of infection by H.somnus and V.parahaemolyticus, respectively. AMPylation occurs in the effector region and leads to inactivation of the GTPase activity by preventing the interaction with downstream effectors, thereby inhibiting actin assembly in infected cells. It is unclear whether some human enzyme mediates AMPylation; FICD has such ability in vitro but additional experiments remain to be done to confirm results in vivo. Ubiquitinated by the BCR(BACURD1) and BCR(BACURD2) E3 ubiquitin ligase complexes, leading to its degradation by the proteasome, thereby regulating the actin cytoskeleton and cell migration.
Cellular localization
細胞内局在性
Cell membrane. Cytoplasm > cytoskeleton.

RhoC
Function
機能
Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers.
Sequence similarities
配列類似性
  • Belongs to the small GTPase superfamily. Rho family.
Cellular localization
細胞内局在性
Cell membrane.

RhoGDI
Function
機能
Regulates the GDP/GTP exchange reaction of the Rho proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them.
Sequence similarities
配列類似性
  • Belongs to the Rho GDI family.
Cellular localization
細胞内局在性
Cytoplasm.

関連リンク


注目ターゲット




Alexa Fluor® is a registered trademark of Life Technologies. Alexa Fluor® dye conjugates contain(s) technology licensed to Abcam by Life Technologies.

GST ~ 注目ターゲットのおすすめ製品


ウサギ・モノクローナル 抗体

ターゲット名

交差種

適用・アプリケーション

文献

Abreview
数 評価

製品番号

GSTICC, IP, WB ab111947
GST3 / GST pi Hu, Ms, RatICC/IF, IHC-P, WB1 ab138491
GST3 / GST pi (HRP) HuIHC-P, WB ab202930
GST3 / GST pi
Alexa Fluor® 488
HuICC/IF ab203152

抗体

ターゲット名

交差種

適用・アプリケーション

文献

Abreview
数 評価

製品番号

GSTRb-PN/AChIP, ELISA, ICC, IP, WB105ab19256
GSTMs-MN/ADot, ELISA, ICC, ICC/IF, IP, WB ab18183
GSTMs-M n/aIP, WB92ab92
GSTRb-PELISA, ICC/IF, IP, WB262 ab9085
GST (HRP)Rb-P n/aELISA, WB153 ab3416
GST3 / GST piRb-PHu, Ms, RatICC/IF, IHC-P, WB14ab153949
GST3 / GST piGoat-PHu, Ms, Rat, Hm, RbICC/IF, IHC-P, IP, WB12 ab53943
抗体一覧

タンパク質・ペプチド

製品名

由来

適用・アプリケーション

製品番号

GST protein (Active) S. japonicum FuncS, SDS-PAGEab168026
GST protein(Recombinant)HuFuncS, SDS-PAGEab81793
GST protein(Recombinant)S. japonicumSDS-PAGEab70456
GST3 / GST pi protein(Recombinant)MsMS, SDS-PAGEab206784
GST3 / GST pi protein(Recombinant)HuSDS-PAGE, WBab86703
タンパク質・ペプチド一覧

キット

製品名

交差種

容量

製品番号

GST Activity Assay Kit (Colorimetric) Hu100testab65326
GST Activity Assay Kit (Fluorometric)100testab65325
GST 6XHis-tag ELISA Kit1×96testsab128573
GST tag ELISA Kit1×96testsab126581
キット一覧

生理活性物質

製品名

解説

製品番号

16-OxokahweolSynthetic kahweol derivative. Glutathione S-transferase inducer.ab143677
S-HexylglutathioneGlutathione S-transferase inhibitorab143631
Ethacrynic acidPotent Glutathione S-transferase (GST) inhibitorab141399

GST について

GST
Relevance
関連性
GST (Glutathione S-Transferase) is a 26kDa protein encoded by the parasitic helminth Schistosoma japonicum and widely used in the pGEX family of GST plasmid expression vectors as a fusion protein with foreign proteins.

GST3 / GST pi
Function
機能
Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.
Sequence similarities
配列類似性
  • Belongs to the GST superfamily. Pi family.
  • Contains 1 GST C-terminal domain.
  • Contains 1 GST N-terminal domain.

関連リンク


注目ターゲット

エピトープ・タグ抗体

エピトープ・タグ抗体とレポーター・タンパク質抗体

アフィニティ精製とは?


Alexa Fluor® is a registered trademark of Life Technologies. Alexa Fluor® dye conjugates contain(s) technology licensed to Abcam by Life Technologies.

PLC ~ 注目ターゲットのおすすめ製品


ウサギ・モノクローナル 抗体

ターゲット名

交差種

適用・アプリケーション

文献

Abreview
数 評価

製品番号

Phospholipase C beta 1 Hu, Ms, RatIHC-P, IP, WB ab182368
Phospholipase C beta 3 HuIHC-P, WB ab124735
Phospholipase C beta 1+Phospholipase C beta 3 Hu, Ms, RatIHC-P, IP, WB ab184743
Phospholipase C gamma 1 Hu, Ms, RatICC, IP, WB ab76155
Phospholipase C gamma 1
(HRP)
HuWB ab195861
Phospholipase C gamma 1
(pY783)
HuICC, WB ab76031
Phospholipase C gamma 1 (pY1253) HuICC, WB ab81284
PLCG 2 HuFlow Cyt, IHC-P, IP, WB ab133522
PLCG 2
Alexa Fluor® 488
Hu ICC/IF ab201241

抗体

ターゲット名

交差種

適用・アプリケーション

文献

Abreview
数 評価

製品番号

Phospholipase C beta 1Rb-P Ms, Rat, HuICC/IF, IHC-P, WB ab185724
Phospholipase C beta 3Rb-PHuELISA, IHC-P, WB22 ab52199
Phospholipase C beta 3 (pS537)Rb-P Ms, HuELISA, ICC/IF, IHC-P, WB3 ab73998
Phospholipase C gamma 1Ms-M
Hu, Ms, RatELISA, Flow Cyt, IP, WB31 ab16955
Phospholipase C gamma 1Ms-M
HuELISA, Flow Cyt, ICC/IF, IP, WB13 ab41433
Phospholipase C gamma 1Rb-P
HuICC/IF, WB ab107455
PLCG 2 Ms-M
HuICC, WB ab201645
抗体一覧

タンパク質・ペプチド

製品名

由来

適用・アプリケーション

製品番号

Phospholipase C beta 1 protein (Recombinant )HuELISA, WBab153213
Phospholipase C gamma 1 protein (Recombinant )HuELISA, SDS-PAGE, WBab112339
Phospholipase C gamma 1 (pY775) peptideHuBL, ELISAab42756
タンパク質・ペプチド一覧

生理活性物質

製品名

解説

製品番号

U 73122Standard phospholipase C (PLC) inhibitorab120998
ManoalideIrreversible phospholipase C (PLC) inhibitorab141554
L-alpha-PhosphatidylinositolPhospholipase C substrateab146179
生理活性物質一覧

PLC (ホスフォリパーゼ C)について

Phospholipase C beta 1
Function
機能
二次メッセンジャー分子であるジアシルグリセロール(DAG)およびイノシトール1,4,5-トリホスフェート(IP3)の産生は、活性化ホスファチジルイノシトール特異的ホスホリパーゼ C による触媒で行われる。
Involvement in disease
関連疾患
てんかん性脳症、 早産
Sequence similarities
配列類似性
  • C2 ドメイン
  • PI-PLC X-box ドメイン
  • PI-PLC Y-box ドメイン
Cellular localization
細胞内局在性
核膜。細胞質。心筋細胞の核膜で アドレナリン受容体(ADREN1A、ADREN1B)と共局在。

Phospholipase C beta 3
Function
機能
二次メッセンジャー分子であるジアシルグリセロール(DAG)およびイノシトール1,4,5-トリホスフェート(IP3)の産生は、活性化ホスファチジルイノシトール特異的ホスホリパーゼ C による触媒で行われる。
Sequence similarities
配列類似性
  • C2 ドメイン
  • PI-PLC X-box ドメイン
  • PI-PLC Y-box ドメイン
Cellular localization
細胞内局在性
細胞膜。

Phospholipase C gamma
Function
機能
アクチン再編成および細胞遊走において機能する。二次メッセンジャー分子であるジアシルグリセロール(DAG)およびイノシトール1,4,5-トリホスフェート(IP3)の産生は、活性化ホスファチジルイノシトール特異的ホスホリパーゼ C による触媒で行われる。ヘパリン結合成長因子1(酸性線維芽細胞増殖因子)活性化チロシンキナーゼの主な基質。
Sequence similarities
配列類似性
  • C2 ドメイン
  • EF-hand ドメイン
  • PH ドメイン(二つ)
  • PI-PLC X-box ドメイン
  • PI-PLC Y-box ドメイン
  • SH2 ドメイン(二つ)
  • SH3 ドメイン
Post-translational modifications
翻訳後修飾
受容体媒介性 PLC-gamma-1 および PLC-gamma-2 の活性化は、様々な成長因子受容体や免疫系受容体に応答するチロシンキナーゼがそれらをリン酸化することにより行われる。PTPRJ によって脱リン酸化されることがある。活性化 T 細胞において、CBLB によってユビキチン化される。
Cellular localization
細胞内局在性
細胞突起 > 葉状仮足(lamellipodium)、細胞突起 > ひだ(ruffle)、EGF (Epidermal growth factor)処理により、 葉状仮足(lamellipodium)やひだ(ruffle)に素早く分配される。

PLCG 2
Function
機能
二次メッセンジャー分子であるジアシルグリセロール(DAG)およびイノシトール1,4,5-トリホスフェート(IP3)の産生は、活性化ホスファチジルイノシトール特異的ホスホリパーゼ C による触媒で行われる。膜を通過するシグナルにとって非常に重要な酵素。
Sequence similarities
配列類似性
  • C2 ドメイン
  • PH ドメイン
  • PI-PLC X-box ドメイン
  • PI-PLC Y-box ドメイン
  • SH2 ドメイン(二つ)
  • SH3 ドメイン
Post-translational modifications
翻訳後修飾
様々な成長因子受容体や免疫系受容体のリガンドによる活性化でチロシン残基がリン酸化され、ホスフォリパーゼ活性が活性化される。

関連リンク


Neuroscience
ポータル

注目ターゲット

代謝型グルタミン酸受容体 mGluR


Alexa Fluor® is a registered trademark of Life Technologies. Alexa Fluor® dye conjugates contain(s) technology licensed to Abcam by Life Technologies.

CREB ~ 注目ターゲットのおすすめ製品


ウサギ・モノクローナル 抗体

ターゲット名

交差種

適用・アプリケーション

文献

Abreview
数 評価

製品番号

CREB Hu, Ms, RatFlow Cyt, ICC/IF, IHC-P, IP, WB143 ab32515
CREB (pS133)Hu, Ms, RatICC/IF, IHC-P, IP, WB1513 ab32096
CREB (HRP) HuIHC-P ab194313
CREB
Alexa Fluor® 647
HuFlow Cyt, ICC/IF ab194312
CREB (pS133)
Alexa Fluor® 405
HuICC/IF ab210124

抗体

ターゲット名

交差種

適用・アプリケーション

文献

Abreview
数 評価

製品番号

CREBMs-M
ELISA, ICC/IF, IHC-P, IP, WB ab178322
CREBRb-P Ms, Rat, HuChIP, ELISA, ICC/IF, IHC-P, WB53 ab31387
CREB (pS129+S133)Rb-PHu, MsWB22 ab10564
CREB FITCRb-MFlow Cyt ab175642
抗体一覧

タンパク質・ペプチド

製品名

由来

適用・アプリケーション

製品番号

CREB protein RatFuncSab43602
CREB peptideFuncS, HPLCab204856
CREB proteinHuSDS-PAGEab176930
CREB (pS133) peptideHuBLab38313
タンパク質・ペプチド一覧

キット

製品名

交差種

容量

製品番号

CREB1 ELISA Kit Hu, Ms1×96testsab176639
CREB1 pS133 + CREB1 ELISA Kit Ms, Hu1×96testsab176659
CREB1 pS133 ELISA Kit Hu, Ms1×96testsab176638
Total CREB Transcription Factor Assay KitMs, Rat, Hu, Mkey5×96tests
1×96tests
ab207201
キット一覧

抗体アレイ

製品名

交差種

容量

製品番号

MAPK Phosphorylation 抗体 Array

Targets: Akt (pS473), CREB (pS133), ERK1 (pT202/Y204)/ERK2 (pT185/Y187), GSK3a (pS21), GSK3b (pS9), HSP27 (pS82), JNK (pT183), MEK (pS217/221), MKK3 (pS189), MKK6 (pS207), MSK2 (pS360), mTOR (pS2448), p38 (pT180/Y182), p53 (pS15), P70S6K (pT421/S424), RSK1 (pS380), RSK2 (pS386).

Hu2 Membrane
4 Membrane
8 Membrane
ab211061

CREB について

Function
機能
CREB タンパク質は、ウイルスや細胞のプロモーターに存在する配列である cAMP 応答エレメント(CRE)に結合し転写を活性化する。転写活性化は、Ser-133 リン酸化とは独立して機能する TORC コアクチベーターによって増強される。概日リズムの同期にも関与している。
Involvement in disease
関連疾患
CREB1 の欠損は、血管腫性線維性組織球腫(AFH)の原因となり得る。通常、小児および青年で起こり、結節性皮下成長によって現れる悪性線維性組織球腫の特徴的なバリアント。 血管腫性線維性組織球腫患者では、CREB1 を含む染色体異常が見られる。CREB1 のトランスロケーション t(2; 22)(q33; q12)は、この腫瘍において最も一般的な遺伝的異常である EWSR1 / CREB1 融合遺伝子を生成する。
Sequence similarities
配列類似性
  • bZIP ファミリーに属する。
  • 1つの bZIP ドメインを有する。
  • 1つの KID (kinase-inducible) ドメインを有する。
Post-translational modifications
翻訳後修飾
リン酸化されることにより活性化される。 Ser-133 および Ser-142 の両方のリン酸化は、CREBの活性を調節し、概日リズムに関与する。Ser-133 のリン酸化は CREBBP の結合を可能にする。ATM または ATR によって、DNA 損傷時にリン酸化されることが示唆されている。SUMO1 によって SUMO 化される。このタンパク質の核局在化のためには、Lys-304 上の SUMO 化が必須であるが、Lys-285 上の SUMO 化は必要ではない。 SUMO 化は、低酸素下で増強され、核局在および安定化を促進する。
Cellular localization
細胞内局在性

関連リンク


注目ターゲット

Epigenetics
ポータル


MMP ~ 注目ターゲットのおすすめ製品


ウサギ・モノクローナル 抗体

ターゲット名

交差種

適用・アプリケーション

文献

Abreview
数 評価

製品番号

MMP1 HuFlow Cyt, ICC, IHC-Fr, IHC-P, WB123 ab52631
MMP2Hu, Ms, RatFlow Cyt, ICC/IF, WB16 1ab92536
MMP3Hu, Ms, RatICC/IF, IHC-P, WB233 ab52915
MMP7 HuIHC-P, WB2 ab205525
MMP8Hu, Ms, RatFlow Cyt, ICC, IHC-P, IP, WB103 ab81286
MMP9 Rat, HuFlow Cyt, ICC/IF, IHC-P, WB82 6ab76003
MMP9 Hu, DogIHC-Fr, IHC-P, WB105ab137867
MMP9 (HRP) HuIHC-P, WB ab194316
MMP12Hu, Ms, RatIP, WB14 ab52897
MMP14 Ms, Rat, HuFlow Cyt, ICC/IF, IHC-P, IP, WB3113 ab51074
MMP14 (HRP) HuIHC-P ab194242
MMP17Hu, Ms, RatICC/IF, IHC-FoFr, IHC-P, WB52 ab51075

抗体

ターゲット名

交差種

適用・アプリケーション

文献

Abreview
数 評価

製品番号

MMP1Rb-PHu, Dog, HrsICC/IF, IHC-Fr, IHC-P, WB106 ab38929
MMP2Rb-PHu, Ms, Rat, ChkFlow Cyt, ICC/IF, IHC-Fr, IHC-P, WB5013 ab37150
MMP2Ms-MHu, Ms, RatIHC-FoFr, IHC-P, WB184 ab86607
MMP3Rb-PHu, Ms, RatELISA, ICC/IF, IHC-P, WB156ab53015
MMP7Rb-PHu, MsELISA, ICC/IF, IHC-Fr, IHC-P, IP, WB92 ab5706
MMP9Rb-PHu, Ms, Rat, DogELISA, ICC, ICC/IF, IHC-FoFr, IHC-Fr, IHC-P, IP, WB132 42ab38898
MMP10Rb-PHu, Ms, RatELISA, Flow Cyt, ICC/IF, IHC-Fr1 1ab59437
MMP12Rb-PHuICC/IF, IHC-P, WB1 ab137444
MMP14Rb-PHu, Ms, PigELISA, ICC/IF, IHC-P, IP, WB193 ab38971
MMP14Ms-MHu, MsELISA, Flow Cyt, ICC/IF, IHC-Fr, Inhib, IP, WB5 ab78738
MMP19Rb-PHuELISA, IHC-P, WB3 ab53146

タンパク質・ペプチド

製品名

由来

適用・アプリケーション

文献数

製品番号

MMP1 protein (Active)HuFuncS, SDS-PAGEab134442
MMP1 proteinHuELISA, SDS-PAGE, WBab39297
MMP2 protein (Recombinant / Active)HuFuncS, SDS-PAGE1ab174022
MMP3 protein (Recombinant / Active)HuFuncS, SDS-PAGE, WB1ab96555
MMP3 proteinHuSDS-PAGE, WB1ab39306
MMP9 protein (proenzyme / Active)HuFuncS, SDS-PAGE, WB1ab157344
MMP9 protein (Active)HuFuncS, SDS-PAGE, WB1ab168863
MMP9 protein (Recombinant)MsFuncS, SDS-PAGE, WB2ab39309
MMP11 protein (Recombinant)HuELISA, MS, SDS-PAGE, WB1ab92861
MMP12 protein (Recombinant)HuELISA, SDS-PAGE, WBab80209
MMP13 protein (Recombinant / Active)HuFuncS, SDS-PAGEab134452
MMP14 protein (Recombinant / Active)HuFuncS, SDS-PAGEab168081
MMP24 protein (Recombinant / Active)HuFuncS, SDS-PAGEab157081
タンパク質・ペプチド一覧

キット

製品名

交差種

容量

製品番号

MMP14 ELISA Kit Hu1×96testsab197747
MMP1 ELISA Kit Hu1×96testsab215083
MMP3 ELISA Kit Hu1×96testsab189572
MMP3 ELISA Kit Ms1×96testsab203363
MMP8 ELISA Kit Ms1×96testsab206982
MMP8 ELISA Kit Hu1×96testsab219050
MMP13 ELISA Kit Rat, Rb, Cow, Hu, Pig1×96testsab221839
MMP-3 Activity Assay Kit (Fluorometric)Mamm100testab118972
MMP1 Inhibitor Screening Assay Kit (Colorimetric)1×96testsab139443
MMP7 Inhibitor Screening Assay Kit (Colorimetric)1×96testsab139444
MMP9 Inhibitor Screening Assay Kit (Colorimetric)1×96testsab139448
MMP2 Inhibitor Screening Assay Kit (Colorimetric)1×96testsab139446
MMP8 Multiplex Immunoassay Kit Ms1×96tests ab221531
MMP8 Multiplex Immunoassay Kit Hu1×96testsab219031
MMP13 Multiplex Immunoassay Kit Hu1×96testsab219030
キット一覧

抗体アレイ

製品名

交差種

容量

製品番号

MMP Antibody Array - Membrane (10 Targets)
Targets:MMP-1, MMP-2, MMP-3, MMP-8, MMP-9, MMP-10, MMP-13, TIMP-1, TIMP-2, TIMP-4
Hu1×4Membranes
1×8Membranes
ab134004
MMP Antibody Array (10 Targets) - Quantitative
Targets:MMP-1, MMP-2, MMP-3, MMP-8, MMP-9, MMP-10, MMP-13, TIMP-1, TIMP-2, TIMP-4.
Hu8test
22test
50test
ab197453
Angiogenesis Antibody Array - Membrane (23 targets)
Targets: Angiopoietin 1, Angiopoietin 2, Angiostatin, Endostatin, G-CSF, GM-CSF, I-309, IL-10, IL-1 alpha, IL-1 beta, IL-2, IL-4, I-TAC, MCP-3, MCP-4, MMP-1, MMP-9, PECAM-1, Tie-2, TNF alpha, uPAR, VEGFR2, VEGFR3
Hu1×4Membranes
1×8Membranes
ab169808
Cytokine Array – Rat Cytokine Antibody Array (Membrane, 34 Targets)
Targets: Activin A, Agrin, Thymus Chemokine-1, B7-2/CD86, CINC-1, CINC-2alpha, CINC-3, CNTF, Fas Ligand, Fractalkine, GM-CSF, ICAM-1, IFN-gamma, IL-1 R6, IL-10, IL-13, IL-1alpha, IL-1beta, IL-2, IL-4, IL-6, Leptin, LIX, L-Selectin, MCP-1, MIP-3alpha, MMP-8, beta-NGF, PDGF-AA, Prolactin R, RAGE, TIMP-1, TNF-alpha, VEGF.
Rat1×4Membranes
1×8Membranes
ab133992
Cytokine Antibody Array - Membrane (34 Targets)
Targets: bFGF, DPPIV/CD26, Dtk, E-Selectin, Fc gamma RIIB, Flt-3 Ligand, GITR, HGF R, ICAM-1, IGFBP-2, IGF-1, IGF-2, IL-15, IL-17B R, IL-7, I-TAC, Lungkine, MDC, MMP-2, MMP-3, Osteopontin, Osteoprotegerin, Pro-MMP-9, Resistin, Shh-N, Thymus CK-1, TIMP-2, TRANCE, TROY, TSLP, VEGFR1, VEGFR2, VEGFR3, VEGF-D
Ms1×2Membranes
1×4Membranes
1×8Membranes
ab169810
Cytokine Antibody Array - Membrane (51 Targets)
Targets: Adipsin, BCAM, CD30, CD40, Fc gamma RIIB/C, Ferritin, FLRG, Follistatin, Furin, Galectin-7, GDF-15, Growth Hormone, IL-10 R alpha, IL-22, IL-28A, IL-29, IL-31, Insulin, Luteinizing hormone, LIMPII, LYVE-1, Marapsin, MICA, MICB, MMP-2, MMP-7, MMP-8, MMP-10, NCAM-1, Nidogen-1, NrCAM, NRG1-beta 1, Osteopontin, PAI-I, Platelet factor 4, PSA-total, RAGE, RANK, Resistin, SAA, Siglec-9, TACE, TIM-1, TRAIL R2, Trappin-2, TREM-1, TSH, TSLP, VCAM-1, VEGF-C, XEDAR
Hu1×2Membranes
1×4Membranes
1×8Membranes
ab169815
キット一覧

生理活性物質

製品名

解説

製品番号

MMP-9 inhibitorMMP-9 inhibitor. Role in tumor cell migration, invasion and angiogenesisab142180
MMP-2/MMP-9 Inhibitor IMatrix metalloproteinase inhibitor of MMP-2/MMP-9. Synergizes with TNF-α to induce apoptosis in cancer cells. Active in vitro.ab145190
beta-ElemeneAnticancer agent. Induces G2M cell cycle arrest and apoptosis. Induces caspase-3, -7 and -9 activities. Decreases Bcl-2 expression. Induces mitochondrial release of cytochrome c. Inhibits microtubule polymerization. Downregulates uPA, uPAR, MMP-2, and MMP-9 expression. Shows antiproliferative effects. Shows antitumor effects in vivo. ab145171
Batimastat (BB-94)Potent, broad-spectrum MMP inhibitor (IC50 values are 3 (MMP-1), 4 (MMP-2), 4 (MMP-9), 6 (MMP-7), 20 nM (MMP-3)). Anticancer activity in vivo.ab142087
CL-82198Selective MMP-13 inhibitor (IC50 = 3.2 μM). Displays no activity against MMP-1, MMP-9 or TACE. Active in vivo.ab141576
生理活性物質一覧

ライゼート

製品名

製品番号

MMP1 293T Transfected Lysate - (positive control)ab94053
MMP10 293T Transfected Lysate - (positive control)ab94294

関連リンク


注目ターゲット

Cancer
ポータル

MMP9

MMP-9 発現誘導プロトール

ゼラチナーゼ活性の測定

APC ~ 注目ターゲットのおすすめ製品


ウサギ・モノクローナル 抗体

ターゲット名

交差種

適用・アプリケーション

文献

Abreview
数 評価

製品番号

APC Rat, HuICC, IHC-P, IP, WB52 ab40778
APC (HRP) HuIHC-P ab193785

抗体

ターゲット名

交差種

適用・アプリケーション

文献

Abreview
数 評価

製品番号

APCRb-PHu, Ms, XlICC/IF, IHC-P, IP, WB106 ab15270
APCMs-MHu, Ms, Rat, MkFlow Cyt, ICC/IF, IHC-Fr, IHC-FrFl, IHC-P152 ab16794
APC (pS2054)Rb-PHuELISA, IHC-P1 ab59421
APC (HRP)Ms-M HuIHC-Fr, IP, WB ab187799
抗体一覧

APC について

Function
機能
Tumor suppressor. Promotes rapid degradation of CTNNB1 and participates in Wnt signaling as a negative regulator. APC activity is correlated with its phosphorylation state. Activates the GEF activity of SPATA13 and ARHGEF4. Plays a role in hepatocyte growth factor (HGF)-induced cell migration. Required for MMP9 up-regulation via the JNK signaling pathway in colorectal tumor cells. Acts as a mediator of ERBB2-dependent stabilization of microtubules at the cell cortex. It is required for the localization of MACF1 to the cell membrane and this localization of MACF1 is critical for its function in microtubule stabilization.
Tissue specificity
組織特異性
Expressed in a variety of tissues.
Involvement in disease
関連疾患
Defects in APC are a cause of familial adenomatous polyposis (FAP) [MIM:175100]; which includes also Gardner syndrome (GS). FAP and GS contribute to tumor development in patients with uninherited forms of colorectal cancer. FAP is characterized by adenomatous polyps of the colon and rectum, but also of upper gastrointestinal tract (ampullary, duodenal and gastric adenomas). This is a viciously premalignant disease with one or more polyps progressing through dysplasia to malignancy in untreated gene carriers with a median age at diagnosis of 40 years. Defects in APC are a cause of hereditary desmoid disease (HDD) [MIM:135290]; also known as familial infiltrative fibromatosis (FIF). HDD is an autosomal dominant trait with 100% penetrance and possible variable expression among affected relatives. HDD patients show multifocal fibromatosis of the paraspinal muscles, breast, occiput, arms, lower ribs, abdominal wall, and mesentery. Desmoid tumors appears also as a complication of familial adenomatous polyposis. Defects in APC are a cause of medulloblastoma (MDB) [MIM:155255]. MDB is a malignant, invasive embryonal tumor of the cerebellum with a preferential manifestation in children. Although the majority of medulloblastomas occur sporadically, some manifest within familial cancer syndromes such as Turcot syndrome and basal cell nevus syndrome (Gorlin syndrome). Defects in APC are a cause of mismatch repair cancer syndrome (MMRCS) [MIM:276300]; also known as Turcot syndrome or brain tumor-polyposis syndrome 1 (BTPS1). MMRCS is an autosomal dominant disorder characterized by malignant tumors of the brain associated with multiple colorectal adenomas. Skin features include sebaceous cysts, hyperpigmented and cafe au lait spots. Defects in APC are a cause of gastric cancer (GASC) [MIM:613659]; also called gastric cancer intestinal or stomach cancer. Gastric cancer is a malignant disease which starts in the stomach, can spread to the esophagus or the small intestine, and can extend through the stomach wall to nearby lymph nodes and organs. It also can metastasize to other parts of the body. The term gastric cancer or gastric carcinoma refers to adenocarcinoma of the stomach that accounts for most of all gastric malignant tumors. Two main histologic types are recognized, diffuse type and intestinal type carcinomas. Diffuse tumors are poorly differentiated infiltrating lesions, resulting in thickening of the stomach. In contrast, intestinal tumors are usually exophytic, often ulcerating, and associated with intestinal metaplasia of the stomach, most often observed in sporadic disease. Defects in APC are a cause of hepatocellular carcinoma (HCC) [MIM:114550]. This defect includes also the disease entity termed hepatoblastoma.
Sequence similarities
配列類似性
  • Belongs to the adenomatous polyposis coli (APC) family.
  • Contains 7 ARM repeats.
Post-translational modifications
翻訳後修飾
Phosphorylated by GSK3B. Ubiquitinated, leading to its degradation by the proteasome. Ubiquitination is facilitated by Axin. Deubiquitinated by ZRANB1/TRABID.
Cellular localization
細胞内局在性
Cell junction > adherens junction. Cytoplasm > cytoskeleton. Cell projection > lamellipodium. Cell projection > ruffle membrane. Cytoplasm. Cell membrane. Associated with the microtubule network at the growing distal tip of microtubules. Accumulates in the lamellipodium and ruffle membrane in response to hepatocyte growth factor (HGF) treatment. The MEMO1-RHOA-DIAPH1 signaling pathway controls localization of the phosophorylated form to the cell membrane.

関連リンク


注目ターゲット

Cancer
ポータル

ErbB

More Entries

登録