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バイオ関連ニュース17.9.15

メールマガジン 17.9.13 神経炎症 蛍光標識抗体 ユビキチン

1. 神経炎症とアルツハイマー病ポスター

炎症と神経変性疾患との関係が一目で分かります。

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2. 脳神経科学関連抗体

蛍光色素が直接標識された製品が多数あります。

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3. ユビキチン関連おすすめ製品

Linkage 特異的抗体やアッセイキットなど、豊富なラインアップ。

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関連リンク


アブカムではこのようなメールマガジンを約週 1 回のペースでお送りしています。
  • 購読をご希望の方はメールでお知らせください。
  • メールマガジンのバックナンバーはこちら
  • メールマガジンよりも情報が早いツイッターはこちら

miRNA ポータル

アブカムの FirePlex® マルチプレックス miRNA アッセイの特長

  • 貴重なサンプルを無駄にしません

    血清・血漿、尿などの体液サンプルをたった 20µL から測定できます。体液サンプルからの total RNA 精製は不要です。Circulating マイクロ RNA をダイレクトに測定できます。

  • 幅広いサンプルに対応

    血清や血漿サンプルの場合、採血方法を問わず測定できます。EDTAカリウム、ヘパリンナトリウム、ヘパリンリチウム、クエン酸ナトリウムを用いた採血されたサンプルでそれぞれテスト済みです(詳細はテクニカルノートをご覧ください)。

  • 簡単な操作でマルチプレックス測定

    FirePlex® 独自のパーティクル技術により、最大 68 種類のマイクロ RNA を1 ウェルで同時に測定できます。煩わしい繰り返し操作は不要です。

  • 測定項目の組み合わせは自由

    8 種類の便利なパネル製品をご用意。miRBase 21 に登録されているマイクロ RNA であれば最大 68 種類まで自由に組み合わせ可能です。完全合成マイクロ RNA にも対応できます。

  • 追加のコストは不要

    一般的なフローサイトメーター*で測定でき、専用機器は不要です。解析用専用ソフトウェアは無償でダウンロードできます。
    *受託測定サービスも承っています。
  • *Guava easyCyte Accuri C6, FACSCanto, LSRII and LSRFortessa 等でご使用いただけます。その他の機種についてはお問合せください。
FirePlex® マルチプレックス miRNA アッセイに関するお問い合わせはこちら

図:FirePlex® ワークフロー

ピックアップ情報

MicroRNA profiling direct from biofluids, using Firefly® particle technology Article
FirePlex® は、血清、尿、唾液などの biofulid サンプルから total RNA の精製無しで miRNA を測定できます(英文)
How does the FirePlex® miRNA assay work?  Article
FirePlex® マルチプレックス miRNA アッセイの各ステップの解説です(英文)
主なマイクロ RNA 検出アッセイ法 Article
FirePlex® を、RT-qPCR などの既存テクノロジーと比較しました
Benchmarking the FirePlexᵀᴹ miRNA assays Article
FirePlex® と既存法を比較したテクニカル・ノートがダウンロードできます(英文)
マイクロ RNA のバイオマーカーとしての応用 Article
miRNA のバイオマーカーとしての可能性と最近の知見をご紹介します
MicroRNAs in exosomes Article
エクソソームと miRNA に関する最近の知見をご紹介します
マルチプレックス miRNA サンプル・プロファイリング・サービス Article
サンプルをお預かりしての受託測定サービスも行なっています
感染症関連がんと miRNA Webinar
マルチプレックス・アッセイを用いた miRNA プロファイリングについて解説した Webinar です(英語)
MicroRNA database and target prediction tool index Article
miRNA 関連データベースとターゲット予測ツールの紹介です(英文)
FirePlex® Analysis Workbench: software for FirePlex miRNA assays Webinar
FirePlex® 解析用ソフトウェア(フリーウェア)を解説した Webinar です(英語)

分野別パネル製品

疾患関連パネル
精神・神経疾患関連 Neurology V2 循環器疾患関連 Cardiology V2 がん関連 Oncology V2 免疫疾患関連 Immunology V2
毒性関連パネル
肝毒性用 Liver Tox V2 腎毒性用 Kidney Tox V2 ラット膵臓毒性用
Pancreatic Toxicity
ラット心毒性用
Cardiac Toxicity

使用文献・参考文献

FirePlex® が使用された文献
​Adams BD et al. (2015). miR-34a Silences c-SRC to Attenuate Tumor Growth in Triple Negative Breast Cancer.​ Cancer Res. 2321:2015.
Flowers E, Gadgil M, Auoizerat BE, Kanaya A (2015). Circulating microRNAs associated with glycemic impairment and progression in Asian Indians. Biomarker Research, 3:22.
Flowers E et al. (2015). Circulating microRNA-320a and microRNA-486 predict thiazolidinedione response: Moving towards precision health for diabetes prevention. Metabolism S20026-0495(15)00156-0. 
Glineur SF, De Ron P, Hanon E, Valentin JP, Dremier ​S, da Costa AN.​ ​Paving the route to plasma miR-208a-3p as an acute cardiac injury biomarker: preclinical rat data supports its use in drug safety assessment (2015). Toxicological Sciences, kfv222.
Hoss AG et al. (2015). miR-10b-5p expression in Huntington's disease brain relates to age of onset and the extent of striatal involvement. BMC Med. Genomics 8.1, 10.​
Joerger M et al. (2014). ​​Circulating microRNA profiling in patients with advanced non-squamous NSCLC receiving bevacizumab/erlotinib followed by platinum-based chemotherapy at progression (SAKK 19/05).​
Khodakov D, Wang C, Zhang DY (2016). Diagnostics based on nucleic acid sequence variant profiling: PCR, hybridization, and NGS approaches.​ Adv Drug Deliv Rev. S0169-409X(16)30104-1.
Minh TN. Le Hamar P, Guo C, Basar E, Perdigão-Henriques R, Balaj L, Lieberman J (2014). miR-200 in extracellular vesicles promotes metastasis of breast cancer cells. JCI 124(12), 5109-5128.​
Momen-Heravi F et al. (2015). Increased number of circulating exosomes and their microRNA cargos are potential novel biomarkers in alcoholic hepatitis.​ J Transl Med. ​12;13:261.
Powell J et al. (2016). A cytometry microparticle platform approach for screening tobacco microRNA changes after agrobacterium delivery. Journal of Microbial Methods 127: 230-235.
Ren V, Ambros VR (2015). Caenorhabditis elegans microRNAs of the let-7 family act in innate immune response circuits and confer robust developmental timing against pathogen stress.​ Proc Natl Acad Sci USA 5;112(18).
Ren V et al. (2016). Staufen Negatively Modulates microRNA Activity in Caenorhabditis elegans.​ G3 (Bethesda) ​3;6(5):1227-37.
Sandhu SK, Fassan M, Volinia S, Lovat F, Balatti V, Pekarsky Y, Croce CM (2013) B-cell malignancies in microRNA Eμ-miR-17~92 transgenic mice, PNAS 5, 110(45), 18208.​
Shiu PK, Zhuang JJ, Hunter CP (2014). Assays for direct and indirect effects of C. elegans Endo-siRNAs. Methods Mol. Biol. 1173, 71-87.​
Telonis AG et al. (2015). Dissecting tRNA-derived fragment complexities using personalized transcriptomes reveals novel fragment classes and unexpected dependencies. Oncotarget 22;6(28): 24797-822.
​Zhang Q et al. (2014). Molecular analyses of 6 different types of uterine smooth muscle tumors: Emphasis in atypical leiomyoma. Cancer​ 15;120(20):3165-77.
Zinovyeva AY, Bouasker S, Simard MJ, Hammell CM, Ambros V (2014). Mutations in conserved residues of the C. elegans microRNA Argonaute ALG-1 identify separable functions in ALG-1 miRISC loading and target repression. PLoS Genet. 23, 10(4): e1004286.​
Zou L et al. (2016). Splenic RNA and MicroRNA Mimics Promote Complement Factor B Production and Alternative Pathway Activation via Innate Immune Signaling. J Immunol. ​15;196(6):2788-98.
FirePlex® テクノロジーに関する文献
Appleyard D, Chapin SC, Srinivas R  and Doyle PS (2011). Barcoded hydrogel microparticles for protein detection: synthesis, assay and scanning. Nature Protocols 6, 1761.
Chapin SC and Doyle PS (2011) Ultrasensitive multiplexed microRNA quant on encoded gel microparticles using rolling circle amplification. Analytical Chemistry 83, 7179.
Chapin SC, Pregibon DC and Doyle PS (2009). High-throughput flow alignment of barcoded hydrogel microparticles. Lab on a Chip 9, 3100.​
Chapin SC, Pregibon DC and Doyle PS (2011). Rapid microRNA profiling on encoded gel microparticles. Agnew Chem Int Ed 50, 2289.
Dendukuri D, Pregibon DC, Collins J, Hatton TA and Doyle PS (2006). Continuous-flow lithography for high-throughput microparticle synthesis. Nature Materials 5, ​369.​​
Pregibon DC and Doyle PS (2009). Optimization of encoded hydrogel particles for nucleic acid quantification. Analytical Chemistry 81, 4873.
Pregibon DC, Toner M and Doyle PS (2007). Multifunctional encoded particles for high-throughput biomolecule analysis. Science 315, 1393.​

メールマガジン 17.9.6 イムノアッセイ がん免疫 ES細胞から血球分化

1. マルチプレックスから ELISA へ

イムノアッセイも抗体のエキスパートであるアブカムにお任せください。

記事を読む ≫

2. がん免疫ターゲット

PD-L1 をはじめとした注目ターゲットとその関連製品をまとめました。

製品を探す ≫

3. ヒト ES 細胞から血球細胞への分化誘導

用いるサイトカインと共培養フィーダー細胞をご紹介します。

記事を読む ≫


関連リンク


アブカムではこのようなメールマガジンを約週 1 回のペースでお送りしています。
  • 購読をご希望の方はメールでお知らせください。
  • メールマガジンのバックナンバーはこちら
  • メールマガジンよりも情報が早いツイッターはこちら

Cytokeratin ~ 注目ターゲットのおすすめ製品


ウサギ・モノクローナル 抗体

ターゲット名

交差種

適用・アプリケーション

文献

Abreview
数 評価

製品番号

Cytokeratin 1Hu, MS, Rat WB, IHC-P, IHC-Fr ab185628
Cytokeratin 4HuFlow Cyt, ICC, ICC/IF, IHC-P, IP, WB6 ab51599
Cytokeratin 5Hu, MsIHC-Fr, ICC/IF, Flow Cyt, IHC-P, WB8 ab214586
Cytokeratin 6 HuICC, IHC-P, WB1 ab52620
Cytokeratin 7 Hu, Ms, RatICC/IF, IHC-Fr, IHC-P, WB39ab181598
Cytokeratin 7
BSA and Azide free
Hu, Ms, Rat ICC/IF, WB, IHC-Fr, IHC-P2 ab220804
Cytokeratin 8
BSA and Azide free
Hu, MsWB, IP, IHC-P, Flow Cyt, ICC/IF14 ab217173
Cytokeratin 8+18 Ms, HuFlow Cyt, ICC/IF, IHC-P, IP, WB173 ab53280
Cytokeratin 8 (pS73)Hu, Ms, RatICC/IF, IHC-Fr, IHC-P, WB56 ab32579
Cytokeratin 10Hu, Ms, RatICC/IF, IHC-Fr, IHC-P, WB87 ab76318
Cytokeratin 10
BSA and Azide free
Hu, Ms, Rat ICC/IF, IHC-Fr, WB, IHC-P8ab220806
Cytokeratin 13 Ms, HuICC, IHC-Fr, IHC-P, WB71 ab92551
Cytokeratin 14 Hu, Ms, RatICC/IF, IHC-P, WB23 ab181595
Cytokeratin 14
Alexa Fluor® 488
HuFlow Cyt, ICC/IF2 ab192055
Cytokeratin 15Hu, MsFlow Cyt, ICC/IF, IHC-P, WB81 ab52816
Cytokeratin 16 HuFlow Cyt, ICC, IHC-P, WB91 ab76416
Cytokeratin 17Hu, Ms, RatFlow Cyt, ICC/IF, IHC-P, WB3 ab109725
Cytokeratin 18 HuFlow Cyt, ICC/IF, IHC-Fr, IHC-P, WB145 ab32118
Cytokeratin 19 Hu, MsFlow Cyt, ICC/IF, IHC-P, WB237 ab52625
Cytokeratin 19
BSA and Azide free
Hu, Ms ICC/IF, Flow Cyt, IHC-P, WB8ab195872
Cytokeratin 20Goat, Hu, Mmst, Pig, RatFlow Cyt, ICC, IHC-Fr, IHC-P, IP, WB913 ab76126
Cytokeratin 20
BSA and Azide free
Goat, Hu, Mmst, Pig, Rat Flow Cyt, ICC/IF, IP, IHC-Fr, WB, IHC-P7ab219589

抗体

ターゲット名

交差種

適用・アプリケーション

文献

Abreview
数 評価

製品番号

Acidic CytokeratinMs-M Hu, Ms, Cow, Dog, Rb, Rat, Chk, Turt, MkFlow Cyt, ICC/IF, IHC-Fr, IHC-P, WB ab187381
Cytokeratin 1Ms-MMs, Rat, Chk, Cow, Mk, Rb, TurtIHC-Fr, IHC-P, WB92 ab9286
Cytokeratin 2Ms-M Ms, Rat, Rb, Chk, Cow, Hu, Mk, PigEM, IHC-Fr, IHC-P, WB ab16111
Cytokeratin 3Ms-MHu, Cow, RbFlow Cyt, ICC/IF, IHC-P, WB51 ab77869
Cytokeratin 3+12Ms-MHu, Ms, Cow, RbICC/IF, IHC-Fr, IHC-P, WB31 ab68260
Cytokeratin 4Ms-MHu, Cat, Dog, ZfshFlow Cyt, ICC, WB6 ab9004
Cytokeratin 5Rb-PHu, Ms, Rat, PigELISA, Flow Cyt, ICC/IF, IHC-FoFr, IHC-Fr, IHC-P, WB174 ab53121
Cytokeratin 5 + 6Ms-MHuIHC-P41 ab17133
Cytokeratin 5 + 8Ms-MHu, Ms, Rat, Dog, Hm, Pig, RbDot, Flow Cyt, ICC/IF, IHC-Fr, IHC-P, WB5 ab9005
Cytokeratin 6Ms-MHu, MsFlow Cyt, ICC, IHC-Fr, IHC-P, WB3 ab18586
Cytokeratin 7Ms-MHu, Ms, Rat, Cat, Dog, Goat, Hm, Pig, ZfshFlow Cyt, ICC, ICC/IF, IHC-Fr, IHC-P, WB2312 ab9021
Cytokeratin 8Ms-MHu, Rat, RbFlow Cyt, ICC, ICC/IF, IHC-Fr, IHC-P, WB139 ab9023
Cytokeratin 8+18Ms-MHuFlow Cyt, ICC/IF, IHC-P, WB11 ab17139
Cytokeratin 10Ms-MHu, Ms, Cat, Dog, ShpFlow Cyt, ICC, IHC-Fr, IHC-P, WB133 ab9026
Cytokeratin 10 + 13Ms-M Cat, Hu, Zfsh, DogFlow Cyt, ICC, IHC-Fr, IHC-P, WB12 ab9383
Cytokeratin 13Ms-MHu, Ms, RbICC/IF, IHC-P, WB102 ab16112
Cytokeratin 15Ms-MHu, Ms, Rat, CowFlow Cyt, ICC/IF, IHC-P22 ab80522
Cytokeratin 17Rb-PHu, Ms, RatELISA, ICC, ICC/IF, IHC-P, WB81 ab53707
Cytokeratin 18Ms-M Ms, Rat, Shp, Goat, Hrs, Hm, Cow, Dog, Hu, Pig, Mmst, CatFlow Cyt, ICC, ICC/IF, IHC-Fr, IHC-P, IP, WB4219 ab668
Cytokeratin 19Ms-MHuELISA, Flow Cyt, ICC, ICC/IF, IHC-Fr, IHC-P, IP, WB167 ab7754

タンパク質・ペプチド

製品名

由来

適用・アプリケーション

製品番号

Cytokeratin 1 protein HuELISA, SDS-PAGE, WBab114282
Cytokeratin 7 proteinHuELISA, SDS-PAGE, WBab132933
Cytokeratin 8 proteinHuSDS-PAGEab156970
Cytokeratin 10 protein HuELISA, SDS-PAGE, WBab114223
Cytokeratin 13 proteinHuELISA, SDS-PAGE, WBab112315
Cytokeratin 16 protein HuELISA, SDS-PAGE, WBab114405
Cytokeratin 17 proteinHuELISA, SDS-PAGE, WBab116974
Cytokeratin 18 proteinHuSDS-PAGE, WBab73638
タンパク質・ペプチド一覧

キット

製品名

交差種

容量

製品番号

Cytokeratin 14 Matched 抗体 Pair KitHu2 x 96 tests
10 x 96 tests
ab221423
キット一覧

ライゼート

製品名

適用・アプリケーション

製品番号

Cytokeratin 4 293T Transfected Lysate - (positive control)WBab94137
Cytokeratin 17 293T Transfected Lysate - (positive control)WBab94233
Cytokeratin 19 293T Transfected Lysate - (positive control)WBab94060

Cytokeratin について

Cytokeratin1
Function
機能
May regulate the activity of kinases such as PKC and SRC via binding to integrin beta-1 (ITB1) and the receptor of activated protein kinase C (RACK1/GNB2L1).
Tissue specificity
組織特異性
The source of this protein is neonatal foreskin. The 67-kDa type II keratins are expressed in terminally differentiating epidermis.
Involvement in disease
関連疾患
  • bullous congenital ichthyosiform erythroderma
  • ichthyosis hystrix Curth-Macklin type
  • palmoplantar keratoderma non-epidermolytic
  • ichthyosis annular epidermolytic
  • palmoplantar keratoderma striate type 3
Cellular localization
細胞内局在性
Cell membrane. Located on plasma membrane of neuroblastoma NMB7 cells.

Cytokeratin2
Function
機能
Probably contributes to terminal cornification.

Cytokeratin3
Tissue specificity
組織特異性
Cornea specific.
Involvement in disease
関連疾患
Meesmann corneal dystrophy

Cytokeratin4
Tissue specificity
組織特異性
Detected in the suprabasal layer of the stratified epithelium of the esophagus, exocervix, vagina, mouth and lingual mucosa, and in cells and cell clusters in the mucosa and serous gland ducts of the esophageal submucosa (at protein level). Expressed widely in the exocervix and esophageal epithelium, with lowest levels detected in the basal cell layer.
Involvement in disease
関連疾患
white sponge nevus of cannon

Cytokeratin5
Involvement in disease
関連疾患
  • epidermolysis bullosa simplex Dowling-Meara type
  • epidermolysis bullosa simplex with migratory circinate erythema
  • epidermolysis bullosa simplex Weber-Cockayne type
  • epidermolysis bullosa simplex Koebner type
  • epidermolysis bullosa simplex with mottled pigmentation
  • Dowling-Degos disease

Cytokeratin6
Tissue specificity
組織特異性
Constitutively expressed in distinct types of epithelia such as those in oral mucosa, esophagus, papillae of tongue and hair follicle outer root sheath.

Cytokeratin7
Function
機能
Blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7).
Tissue specificity
組織特異性
Expressed in cultured epidermal, bronchial and mesothelial cells but absent in colon, ectocervix and liver. Observed throughout the glandular cells in the junction between stomach and esophagus but is absent in the esophagus.
Cellular localization
細胞内局在性
Cytoplasm.

Cytokeratin8
Function
機能
Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
Tissue specificity
組織特異性
Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity.
Cellular localization
細胞内局在性
Cytoplasm. Nucleus, nucleoplasm. Nucleus matrix.

Cytokeratin9
Function
機能
May serve an important special function either in the mature palmar and plantar skin tissue or in the morphogenetic program of the formation of these tissues. Plays a role in keratin filament assembly.
Tissue specificity
組織特異性
Expressed in the terminally differentiated epidermis of palms and soles.
Involvement in disease
関連疾患
palmoplantar keratoderma epidermolytic

Cytokeratin10
Tissue specificity
組織特異性
Seen in all suprabasal cell layers including stratum corneum.
Involvement in disease
関連疾患
  • bullous congenital ichthyosiform erythroderma
  • ichthyosis annular epidermolytic

Cytokeratin13
Tissue specificity
組織特異性
Expressed in some epidermal sweat gland ducts (at protein level) and in exocervix, esophagus and placenta.
Involvement in disease
関連疾患
  • bullous congenital ichthyosiform erythroderma
  • ichthyosis annular epidermolytic

Cytokeratin14
Function
機能
The nonhelical tail domain is involved in promoting KRT5-KRT14 filaments to self-organize into large bundles and enhances the mechanical properties involved in resilience of keratin intermediate filaments in vitro.
Tissue specificity
組織特異性
Detected in the basal layer, lowered within the more apically located layers specifically in the stratum spinosum, stratum granulosum but is not detected in stratum corneum. Strongly expressed in the outer root sheath of anagen follicles but not in the germinative matrix, inner root sheath or hair. Found in keratinocytes surrounding the club hair during telogen.
Involvement in disease
関連疾患
  • epidermolysis bullosa simplex Dowling-Meara type
  • epidermolysis bullosa simplex Weber-Cockayne type
  • epidermolysis bullosa simplex Koebner type
  • epidermolysis bullosa simplex autosomal recessive
  • Naegeli-Franceschetti-Jadassohn syndrome
  • dermatopathia pigmentosa reticularis
Cellular localization
細胞内局在性
Cytoplasm. Nucleus. Expressed in both as a filamentous pattern.

Cytokeratin15
Tissue specificity
組織特異性
Expressed in a discontinuous manner in the basal cell layer of adult skin epidermis, but continuously in the basal layer of fetal skin epidermis and nail. Also expressed in the outer root sheath above the hair bulb in hair follicle (at protein level). Expressed homogeneously in all cell layers of the esophagus and exocervix, but detected in the basal cell layer only of oral mucosa, skin and in the basal plus the next two layers of the suprabasal epithelium of the palate.

Cytokeratin16
Tissue specificity
組織特異性
Expressed in the hair follicle, nail bed and in mucosal stratified squamous epithelia and, suprabasally, in oral epithelium and palmoplantar epidermis. Also found in luminal cells of sweat and mammary gland ducts.
Involvement in disease
関連疾患
  • pachyonychia congenita type 1
  • palmoplantar keratoderma non-epidermolytic focal
  • unilateral palmoplantar verrucous nevus

Cytokeratin17
Function
機能
May play a role in the formation and maintenance of various skin appendages, specifically in determining shape and orientation of hair. May be a marker of basal cell differentiation in complex epithelia and therefore indicative of a certain type of epithelial "stem cells". May act as an autoantigen in the immunopathogenesis of psoriasis, with certain peptide regions being a major target for autoreactive T-cells and hence causing their proliferation. Required for the correct growth of hair follicles, in particular for the persistence of the anagen (growth) state. Modulates the function of TNF-alpha in the specific context of hair cycling. Regulates protein synthesis and epithelial cell growth through binding to the adapter protein SFN and by stimulating Akt/mTOR pathway. Involved in tissue repair.
Tissue specificity
組織特異性
Expressed in the outer root sheath and medulla region of hair follicle specifically from eyebrow and beard, digital pulp, nail matrix and nail bed epithelium, mucosal stratified squamous epithelia and in basal cells of oral epithelium, palmoplantar epidermis and sweat and mammary glands. Also expressed in myoepithelium of prostate, basal layer of urinary bladder, cambial cells of sebaceous gland and in exocervix (at protein level).
Involvement in disease
関連疾患
  • pachyonychia congenita type 2
  • steatocystoma multiplex
Cellular localization
細胞内局在性
Cytoplasm.

Cytokeratin18
Function
機能
Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.
Tissue specificity
組織特異性
Expressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma.
Involvement in disease
関連疾患
Defects in KRT18 are a cause of cirrhosis (CIRRH)
Cellular localization
細胞内局在性
Cytoplasm > perinuclear region.

Cytokeratin19
Function
機能
Involved in the organization of myofibers. Together with KRT8, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
Tissue specificity
組織特異性
Expressed in a defined zone of basal keratinocytes in the deep outer root sheath of hair follicles. Also observed in sweat gland and mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, ectocervical epithelium (at protein level). Expressed in epidermal basal cells, in nipple epidermis and a defined region of the hair follicle. Also seen in a subset of vascular wall cells in both the veins and artery of human umbilical cord, and in umbilical cord vascular smooth muscle. Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma in structures that contain dystrophin and spectrin.

Cytokeratin20
Function
機能
Plays a significant role in maintaining keratin filament organization in intestinal epithelia. When phosphorylated, plays a role in the secretion of mucin in the small intestine.
Tissue specificity
組織特異性
Expressed predominantly in the intestinal epithelium. Expressed in luminal cells of colonic mucosa. Also expressed in the Merkel cells of keratinized oral mucosa; specifically at the tips of some rete ridges of the gingival mucosa, in the basal layer of the palatal mucosa and in the taste buds of lingual mucosa.
Cellular localization
細胞内局在性
Cytoplasm.

関連リンク


注目ターゲット

Cancer
ポータル

上皮間葉転換

Ki67 抗体 ~ 臨床への応用

Ki67


Alexa Fluor® is a registered trademark of Life Technologies. Alexa Fluor® dye conjugates contain(s) technology licensed to Abcam by Life Technologies.

バイオ関連ニュース17.9.5

メールマガジン 17.8.23 サイクリン 細胞増殖 蛍光ウエスタン

1. 細胞周期とサイクリン・レベル

発現パターンを利用し、細胞周期の進行をモニター。

解説を読む ≫

2. 細胞増殖研究ガイド

検出、カウント、解析に必要な技術とツールをご紹介。

ガイドを読む ≫

3. ウエスタンでマルチカラー検出

異なるターゲットのバンドを同時に検出。

解説を読む ≫


関連リンク


アブカムではこのようなメールマガジンを約週 1 回のペースでお送りしています。
  • 購読をご希望の方はメールでお知らせください。
  • メールマガジンのバックナンバーはこちら
  • メールマガジンよりも情報が早いツイッターはこちら

メールマガジン 17.8.2 CD133 iNOS ローディング・コントロール

1. CD133 抗体製品

販売を再開しました。ウサギ・ポリクロに加え、新製品のウサギ・モノクロもご紹介します。

製品を見る ≫

2. iNOS と炎症とアポトーシス

S -ニトロソシステイン、SIRT1 などの関連製品も併せてご紹介します。

製品を見る ≫

3. ローディング・コントロール・ガイド

ウエスタン・ブロッティングにおいては必須のコントロール製品です。

解説を読む ≫


関連リンク


アブカムではこのようなメールマガジンを約週 1 回のペースでお送りしています。
  • 購読をご希望の方はメールでお知らせください。
  • メールマガジンのバックナンバーはこちら
  • メールマガジンよりも情報が早いツイッターはこちら

バイオ関連ニュース17.8.28

Ubiquitin ~ 注目ターゲットのおすすめ製品


ウサギ・モノクローナル 抗体

ターゲット名

交差種

適用・アプリケーション

文献

Abreview
数 評価

製品番号

UbiquitinHu, Ms, RatFlow Cyt, ICC/IF, IHC-P, WB51 ab134953
Ubiquitin (linkage-specific K48) Hu, Ms, Rat ICC/IF, IHC-P, Flow Cyt, WB96 ab140601
Ubiquitin (linkage-specific K63)Hu, Ms, Rat WB, IHC-P, ICC/IF, IHC-FoFr, Flow Cyt43 ab179434
Ubiquitin (linkage-specific K27) Hu, Ms, RatIHC-P, WB, Flow Cyt, ICC/IF ab181537
Ubiquitin (linkage-specific K63) Alexa Fluor® 647 HuFlow Cyt, ICC/IF ab192489
Ubiquitin (linkage-specific K48) Alexa Fluor® 488 HuICC/IF ab204274
Ubiquitin Alexa Fluor® 488 HuICC/IF ab205467
Ubiquitin Alexa Fluor® 647 HuICC/IF ab205468

抗体

ターゲット名

交差種

適用・アプリケーション

文献

Abreview
数 評価

製品番号

UbiquitinRb-PHu, Ms, Rat, AGMkICC/IF, IHC-FoFr, IHC-P, IP, WB39 29ab7780
UbiquitinMs-M Ms, Chk, Cow, Hu, At, Ce, Dm, DCV, RatELISA, ICC/IF, IHC-Fr, IHC-P, WB257ab7254
Ubiquitin (HRP)Ms-M n/aIHC-Fr, IHC-P, WB ab191809
抗体一覧

タンパク質・ペプチド

製品名

由来

適用・アプリケーション

製品番号

Ubiquitin K33 only protein (Recombinant / Active )HuFuncS, MS, Other, SDS-PAGE, WBab157076
Ubiquitin K48 only protein (Recombinant / Active )HuFuncS, MS, Other, SDS-PAGE, WBab157048
Ubiquitin K63 only protein (Recombinant / Active )HuFuncS, MS, Other, SDS-PAGE, WBab157056
Tetra-ubiquitin (linkage-specific K63) protein (biotinylated /Recombinant / Active)HuFuncS, SDS-PAGEab189683
Ubiquitin (mutated K33) protein (Recombinant / Chemical Free)HuConjugation, SDS-PAGEab80742
Poly-ubiquitin (linkage-specific K48) protein (Recombinant / Active )HuFuncS, SDS-PAGEab189706
Tetra-ubiquitin (linkage-specific K11) protein (Recombinant)HuHPLC, WBab180028
Triubiquitin (linkage-specific K48) protein (Recombinant)HuMS, SDS-PAGE, WBab166765
Di-ubiquitin (linkage-specific K11) protein (Recombinant)HuSDS-PAGEab206451
Di-ubiquitin (linkage-specific K48) (mutated K48 R) protein (Recombinant)HuFuncS, SDS-PAGEab207972
タンパク質・ペプチド一覧

キット

製品名

交差種

容量

製品番号

E2-Ubiquitin Conjugation Kit50testab139472
Ubiquitylation Assay Kit20testab139467
Ubiquitylation Assay Kit (HeLa lysate-based)20testab139471
キット一覧

生理活性物質

製品名

解説

製品番号

NSC-632839Nonselective ubiquitin isopeptidase inhibitorab144599
SMER3 Potent and selective ubiquitin C-terminal hydrolase L1 inhibitorab144596
BML-282 Potent and selective ubiquitin C-terminal hydrolase L1 inhibitorab144529
TCIDPotent and selective ubiquitin C-terminal hydrolase-L3 inhibitorab144528
PYR-41 Ubiquitin-activating enzyme (E1) inhibitorab141469
生理活性物質一覧

Ubiquitin について

Relevance
関連性
Function: Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling. Similarity: Belongs to the ubiquitin family. Contains 3 ubiquitin-like domains.
Cellular localization
細胞内局在性
Cell Membrane, Cytoplasmic and Nuclear

関連リンク


注目ターゲット

Ubiquitin chains poster

An Introduction to Post-Translational Modifications Webinar


Alexa Fluor® is a registered trademark of Life Technologies. Alexa Fluor® dye conjugates contain(s) technology licensed to Abcam by Life Technologies.

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