製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to BLNK
  • アプリケーション
    適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide: YNIPVRFIEA TKQYALGRKK NGEEYFGSVA EIIRNHQHSP LVLIDSQNNT KDSTRLKYAV KVS, corresponding to a sequence within amino acids 394-456 of Human BLNK (NP_037446).

  • ポジティブ・コントロール
    • HeLa, HepG2, MOLT4 and Raji cell lysates. HeLa cells (IF)

法規制情報

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 40% Glycerol, 1X PBS, pH 7.0
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab97407 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/3000. Predicted molecular weight: 50 kDa.
ICC/IF 1/100 - 1/200.

ターゲット情報

  • 機能
    Functions as a central linker protein that bridges kinases associated with the B-cell receptor (BCR) with a multitude of signaling pathways, regulating biological outcomes of B-cell function and development. Plays a role in the activation of ERK/EPHB2, MAP kinase p38 and JNK. Modulates AP1 activation. Important for the activation of NF-kappa-B and NFAT. Plays an important role in BCR-mediated PLCG1 and PLCG2 activation and Ca(2+) mobilization and is required for trafficking of the BCR to late endosomes. However, does not seem to be required for pre-BCR-mediated activation of MAP kinase and phosphatidyl-inositol 3 (PI3) kinase signaling. May be required for the RAC1-JNK pathway. Plays a critical role in orchestrating the pro-B cell to pre-B cell transition (By similarity). Plays an important role in BCR-induced B-cell apoptosis.
  • 組織特異性
    Expressed in B-cell lineage and fibroblast cell lines (at protein level). Highest levels of expression in the spleen, with lower levels in the liver, kidney, pancreas, small intestines and colon.
  • 関連疾患
    Defects in BLNK are the cause of agammaglobulinemia type 4 (AGM4) [MIM:613502]. It is a primary immunodeficiency characterized by profoundly low or absent serum antibodies and low or absent circulating B cells due to an early block of B-cell development. Affected individuals develop severe infections in the first years of life.
  • 配列類似性
    Contains 1 SH2 domain.
  • 翻訳後修飾
    Following BCR activation, phosphorylated on tyrosine residues by SYK and LYN. When phosphorylated, serves as a scaffold to assemble downstream targets of antigen activation, including PLCG1, VAV1, GRB2 and NCK1. Phosphorylation of Tyr-84, Tyr-178 and Tyr-189 facilitates PLCG1 binding. Phosphorylation of Tyr-96 facilitates BTK binding. Phosphorylation of Tyr-72 facilitates VAV1 and NCK1 binding. Phosphorylation is required for both Ca(2+) and MAPK signaling pathways.
  • 細胞内局在
    Cytoplasm. Cell membrane. BCR activation results in the translocation to membrane fraction.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AGM4 antibody
    • B cell adapter containing SH2 domain protein antibody
    • B cell adapter containing Src homology 2 domain protein antibody
    • B cell adaptor containing SH2 domain antibody
    • B cell linker antibody
    • B cell linker protein antibody
    • B cell-specific adaptor protein antibody
    • B-cell activation antibody
    • B-cell adapter containing a SH2 domain protein antibody
    • B-cell adapter containing a Src homology 2 domain protein antibody
    • B-cell linker protein antibody
    • BASH antibody
    • Bca antibody
    • BLNK antibody
    • BLNK s antibody
    • BLNK_HUMAN antibody
    • Cytoplasmic adapter protein antibody
    • Ly 57 antibody
    • Ly57 antibody
    • Lymphocyte antigen 57 antibody
    • Lyw 57 antibody
    • Lyw57 antibody
    • MGC111051 antibody
    • SH2 domain-containing leukocyte protein, 65-KD antibody
    • SLP 65 antibody
    • SLP-65 antibody
    • SLP65 antibody
    • Src homology [SH2] domain-containing leukocyte protein of 65 kD antibody
    • Src homology 2 domain containing leukocyte protein of 65 kDa antibody
    • Src homology 2 domain-containing leukocyte protein of 65 kDa antibody
    see all

画像

  • Anti-BLNK antibody (ab97407) at 1/500 dilution + HeLa whole cell lysate at 30 µg

    Predicted band size : 50 kDa
  • ab97407 at 1/200 dilution staining BLNK in HeLa cells by Immunofluorescence, Paraformaldehyde fixed. Lower image is merged with DNA probe.

参考文献

ab97407 has not yet been referenced specifically in any publications.

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