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Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Mouse BLBP.
(Peptide available as ab32422.)
Our Abpromise guarantee covers the use of ab32423 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/200 - 1/1000.|
|ICC/IF||Use a concentration of 5 µg/ml.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 15 kDa (predicted molecular weight: 15 kDa).Can be blocked with Mouse BLBP peptide (ab32422).
Abcam recommends using milk as the blocking agent.
|IHC-Fr||Use at an assay dependent concentration. PubMed: 23675446|
IHC-Fr image of Pax2 staining on zebrafish retina sections using ab32423 (1:100). The sections were paraformaldehyde and permeabilized using Triton-X. Antigen retrieval was performed using Tris-HCl and blocking was perfomed using 5% BSA for 1 hour at 23°C. ab32423 was diluted 1:100 and incubated with the sections for 16 hours at 4°C. The secondary antibody was goat polyclonal to rabbit IgG conjugated to Alexa Fluor 488 (1:1000).