製品の概要

  • 製品名Anti-BLBP antibody
    BLBP 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to BLBP
  • アプリケーション適用あり: IHC-Pmore details
  • 種交差性
    交差種: Human, Xenopus laevis
    交差が予測される動物種: Cow, Pig
  • 免疫原

    Synthetic peptide corresponding to Human BLBP (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).

  • ポジティブ・コントロール
    • Human cerebellum, heart or skeletal muscle tissue.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab110099 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能B-FABP could be involved in the transport of a so far unknown hydrophobic ligand with potential morphogenic activity during CNS development. It is required for the establishment of the radial glial fiber system in developing brain, a system that is necessary for the migration of immature neurons to establish cortical layers.
  • 組織特異性Expressed in brain and other neural tissues.
  • 配列類似性Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.
  • ドメインForms a beta-barrel structure that accommodates the hydrophobic ligand in its interior.
  • 細胞内局在Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • B FABP antibody
    • B-FABP antibody
    • BFABP antibody
    • BLBP antibody
    • Brain lipid binding protein antibody
    • Brain lipid-binding protein antibody
    • Brain-type fatty acid-binding protein antibody
    • DKFZp547J2313 antibody
    • FABP 7 antibody
    • FABP7 antibody
    • FABP7_HUMAN antibody
    • FABPB antibody
    • Fatty Acid Binding Protein 7 antibody
    • Fatty acid binding protein 7 brain antibody
    • Fatty acid binding protein brain antibody
    • Fatty acid-binding protein 7 antibody
    • Fatty acid-binding protein, brain antibody
    • Mammary derived growth inhibitor related antibody
    • Mammary-derived growth inhibitor related antibody
    • MRG antibody
    • OTTHUMP00000017119 antibody
    see all

Anti-BLBP antibody 画像

  • ab110099 at 2.5µg/ml staining BLBP in formalin-fixed, paraffin-embedded Human cerebellum tissue after heat induced antigen retrieval in pH 6.0 citrate buffer.
    After incubation with the primary antibody, the slides were incubated with a biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.
  • ab110099 at 2.5µg/ml staining BLBP in formalin-fixed, paraffin-embedded Human heart tissue after heat induced antigen retrieval in pH 6.0 citrate buffer.
    After incubation with the primary antibody, the slides were incubated with a biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.
  • ab110099 at 2.5µg/ml staining BLBP in formalin-fixed, paraffin-embedded Human skeletal muscle tissue after heat induced antigen retrieval in pH 6.0 citrate buffer.
    After incubation with the primary antibody, the slides were incubated with a biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.
  • ab110099 staining BLBP in Xenopus laevis gindbrain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with Bouin's solution, permeabilized with 0.4% Triton X-100 in 3% normal horse serum and blocked with 3% serum for 2 hours at room temperature; antigen retrieval was by heat mediation in 10mM citrate buffer. Samples were incubated with primary antibody (1/100 in 0.4% Triton X-100 in 3% normal horse serum) for 12 hours at 4°C. An undiluted HRP-conjugated horse anti-goat IgG polyclonal was used as the secondary antibody.

    See Abreview

Anti-BLBP antibody (ab110099) 使用論文

ab110099 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10mM citrate buffer
Sample Xenopus laevis Tissue sections (hindbrain)
Specification hindbrain
Permeabilization Yes - 0.4% Triton X in 3% normal horse serum
Fixative Bouin's
Username

Bharti Katbamna

Verified customer

投稿 Sep 17 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"