製品の概要

  • 製品名Anti-BLAP75 antibody
    BLAP75 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to BLAP75
  • 特異性We have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • アプリケーション適用あり: ICC/IF, IHC-P, WB, IPmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    A region between residues 275 and 300 of human BLAP75 (NP_079221.1)

  • ポジティブ・コントロール
    • Whole cell lysate from HeLa cells. This antibody gave a positive result when used in the following methanol fixed cell lines: HeLa.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab70525 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/2000 - 1/10000. Detects a band of approximately 74 kDa (predicted molecular weight: 70 kDa).
IP Use at 1-4 µg/mg of lysate.

ターゲット情報

  • 機能Essential component of the RMI complex, a complex that plays an important role in the processing of homologous recombination intermediates to limit DNA crossover formation in cells. Promotes TOP3A binding to double Holliday junctions (DHJ) and hence stimulates TOP3A-mediated dissolution. Required for BLM phosphorylation during mitosis. Within the BLM complex, required for BLM and TOP3A stability.
  • 配列類似性Belongs to the RMI1 family.
  • 細胞内局在Nucleus. Forms foci in response to DNA damage.
  • Information by UniProt
  • 参照データベース
  • 別名
    • BLAP75 antibody
    • BLM associated polypeptide 75 kDa antibody
    • BLM associated protein 75 kDa antibody
    • BLM-associated protein of 75 kDa antibody
    • C9orf76 antibody
    • FAAP75 antibody
    • FLJ12888 antibody
    • Homolog of yeast RecQ mediated genome instability 1 antibody
    • RecQ mediated genome instability 1 antibody
    • RecQ-mediated genome instability protein 1 antibody
    • Rmi1 antibody
    • RMI1 homolog antibody
    • RMI1 RecQ mediated genome instability 1 homolog antibody
    • RMI1_HUMAN antibody
    • RP11-346I8 antibody
    see all

Anti-BLAP75 antibody 画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling BLAP75 with ab70525 at 1/200 (1µg/ml). Detection: DAB.
  • ICC/IF image of ab70525 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab70525 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-BLAP75 antibody (ab70525) at 0.04 µg/ml

    Lane 1 : Whole cell lysate from HeLa cells at 50 µg
    Lane 2 : Whole cell lysate from HeLa cells at 15 µg
    Lane 3 : Whole cell lysate from HeLa cells at 5 µg

    Developed using the ECL technique

    Predicted band size : 70 kDa
    Observed band size : 74 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 110 kDa,170 kDa,190 kDa,238 kDa,32 kDa,60 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 3 minutes
  • Detection of Human BLAP75 by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab70525 at 3 µg/mg for IP and at 1 µg/ml for subsequent WB detection.

Anti-BLAP75 antibody (ab70525) 使用論文

This product has been referenced in:
  • Ke Y  et al. PICH and BLM limit histone association with anaphase centromeric DNA threads and promote their resolution. EMBO J 30:3309-21 (2011). WB ; Human . Read more (PubMed: 21743438) »

See 1 Publication for this product

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