製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to BIRC6
  • アプリケーション
    適用あり: ICC/IF, WB, IP, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Chimpanzee, Rhesus monkey, Orangutan
  • 免疫原

    corresponding to BIRC6 (C terminal).
    Database link: Q9NR09

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab19609 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml.
WB 1/5000 - 1/15000. Predicted molecular weight: 528 kDa.
IP Use at 1-4 µg/mg of lysate.
IHC-P 1/2000 - 1/10000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能
    Protects cells from undergoing apoptosis.
  • 組織特異性
    Expressed in brain cancer cells.
  • 配列類似性
    In the C-terminal section; belongs to the ubiquitin-conjugating enzyme family.
    Contains 1 BIR repeat.
  • 翻訳後修飾
    Ubiquitinated. Ubiquitination is mediated by the RNF41 E3 ligase and leads to proteasomal degradation, impairing inhibition of apoptosis.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Apollon antibody
    • Apollon protein antibody
    • Baculoviral IAP repeat containing 6 antibody
    • Baculoviral IAP repeat containing protein 6 antibody
    • Baculoviral IAP repeat-containing protein 6 antibody
    • BIR repeat containing ubiquitin conjugating enzyme antibody
    • BIRC 6 antibody
    • BIRC6 antibody
    • BIRC6_HUMAN antibody
    • BRUCE antibody
    • FLJ13726 antibody
    • FLJ13786 antibody
    • KIAA1289 antibody
    • Ubiquitin conjugating BIR domain enzyme apollon antibody
    • Ubiquitin-conjugating BIR domain enzyme apollon antibody
    see all

画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling BIRC6 with ab19609 at 1/5000 (0.2µg/ml). Detection: DAB.
  • All lanes : Anti-BIRC6 antibody (ab19609) at 1/10000 dilution

    Lane 1 : HeLa whole cell lysate at 20 µg
    Lane 2 : HeLa whole cell lysate at 50 µg
    Lane 3 : HeLa S100 extract at 20 µg
    Lane 4 : HeLa S100 extract at 50 µg


    Predicted band size : 528 kDa

    Detection of Human BIRC6 by western blot.
    Lane 1:  20 µg of HeLa whole cell lysate.
    Lane 2:  50 µg of HeLa whole cell lysate.
    Lane 3:  20 µg of HeLa S100 extract.
    Lane 4:  50 µg of HeLa S100 extract.

    Affinity purified anti-BIRC antibody (ab19609) was used at a 1/10,000 dilution.

  • Detection of human BIRC6 by Immunoprecipitation. Affinity purified ab19609 used at 2mcg/mg lysate (whole cell lysate (10mg) from HeLa cells. The immunoprecipitate was resolved by SDS-PAGE and stained with Coomassie Blue. The band that is BIRC6 was confirmed by mass spectrometry.
  • ICC/IF image of ab19609 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab19609, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

参考文献

This product has been referenced in:

See all 6 Publications for this product

レビューと Q&A

Application
Western blot
Sample
Human Cell lysate - whole cell (Patient fibroblasts)
Gel Running Conditions
Reduced Denaturing (6 %)
Loading amount
15 µg
Specification
Patient fibroblasts
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 Jan 30 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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