Anti-Biotin Ligase Epitope Tag 抗体 (ab106159)

製品の概要

  • 製品名Anti-Biotin Ligase Epitope Tag antibody
  • 製品の詳細
    Rabbit polyclonal to Biotin Ligase Epitope Tag
  • 特異性ab106159 detects the minimal peptide substrate (GLNDIFEAQKIEWH) for the E. coli biotin ligase tag (BirA). The antibody will recognize the BirA tag irrespective of whether the tag is biotinylated.
    Western blot analysis of Bacillus subtilis strains producing either BirA-tagged SpoIIIAH or BirA-tagged SpoIIQ showed strong and specific reactivity to the antisera.
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Other
  • 免疫原

    Synthetic peptide corresponding to Escherichia coli Biotin Ligase Epitope Tag.
    Sequence:

    GLNDIFEAQKIEWH

  • ポジティブ・コントロール
    • Whole cell lysates from Bacillus subtilis producing BirA-tagged SpoIIIAH or BirA-tagged SpoIIQ
  • 特記事項Dilute only prior to immediate use.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • バッファーpH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.88% Sodium chloride, Whole serum
  • 精製度Whole antiserum
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab106159 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/5000.

Anti-Biotin Ligase Epitope Tag antibody (ab106159) 使用論文

ab106159 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Human Cell lysate - nuclear (Hek293T)
Gel Running Conditions Reduced Denaturing (4–15% Mini Protean TGX (Bio-Rad))
Loading amount 30 µg
Treatment Biotin 50 uM for 24 h
Specification Hek293T
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
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投稿 Oct 30 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"