Anti-Biotin Ligase Epitope Tag 抗体 (ab106159)
Key features and details
- Rabbit polyclonal to Biotin Ligase Epitope Tag
- Suitable for: WB
- Isotype: IgG
製品の概要
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製品名
Anti-Biotin Ligase Epitope Tag antibody -
製品の詳細
Rabbit polyclonal to Biotin Ligase Epitope Tag -
由来種
Rabbit -
特異性
ab106159 detects the minimal peptide substrate (GLNDIFEAQKIEWH) of biotin ligase (BirA): E. coli biotin ligase tag (BLT) and biotin acceptor peptide (BAP). The antibody will recognize these tags irrespective of whether the tag is biotinylated.
Western blot analysis of Bacillus subtilis strains producing either BAP-tagged SPoIIIAH or BAP-tagged SPoIIQ showed strong and specific reactivity to the antisera. -
アプリケーション
適用あり: WBmore details -
免疫原
Synthetic peptide corresponding to Escherichia coli Biotin Ligase Epitope Tag.
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ポジティブ・コントロール
- Whole cell lysates from Bacillus subtilis producing BAP-tagged SPoIIIAH or BAP-tagged SPoIIQ
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.88% Sodium chloride, Whole serum -
Concentration information loading...
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精製度
Whole antiserum -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab106159の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (1) |
1/5000.
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特記事項 |
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WB
1/5000. |
データシートおよび資料
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Datasheet download
参考文献 (2)
ab106159 は 2 報の論文で使用されています。
- Chatterjee S et al. Endoplasmic reticulum chaperone genes encode effectors of long-term memory. Sci Adv 8:eabm6063 (2022). PubMed: 35319980
- Johnson BS et al. Biotin tagging of MeCP2 in mice reveals contextual insights into the Rett syndrome transcriptome. Nat Med 23:1203-1214 (2017). WB . PubMed: 28920956