FITC Anti-Biotin 抗体 (ab53469)
Key features and details
- FITC Rabbit polyclonal to Biotin
- Suitable for: ICC/IF
- Reacts with: Species independent
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG
製品の概要
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製品名
FITC Anti-Biotin antibody
Biotin 一次抗体 製品一覧 -
製品の詳細
FITC Rabbit polyclonal to Biotin -
由来種
Rabbit -
標識
FITC. Ex: 493nm, Em: 528nm -
アプリケーション
適用あり: ICC/IFmore details -
種交差性
交差種: Species independent -
免疫原
d-Biotin conjugated to KLH
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特記事項
Fluorescein isothionate; Ab/FITC ratio=approximately 1/20.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Lyophilized:1. Centrifuge the tube at 5000 rpm for 30 seconds to deposit the philosophized powder to the tube bottom; 2. Add 100 uL of distilled water and shake gently for 10 minutes until the powder is completely dissolved; 3. Add 900 uL of 50% glycerol (in distilled water) and shake gently for 5 minutes until all liquid is completely mixed. -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 6
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab53469の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF | (1) |
1/100.
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特記事項 |
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ICC/IF
1/100. |
ターゲット情報
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関連性
Biotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da. -
別名
- Vitamin B4 antibody
画像
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ab53469 staining Biotin in human ovarian cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed with formaldehyde, permeabilized with 0.1% Triton ×100 in PBS and blocking with 5% serum for 30min at 230C was performed. Samples were incubated with primary antibody (1/100) for 1 hour at 23°C.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab53469 は 4 報の論文で使用されています。
- Dziasek K et al. Hybrid seed incompatibility in Capsella is connected to chromatin condensation defects in the endosperm. PLoS Genet 17:e1009370 (2021). PubMed: 33571184
- Rubio K et al. Inactivation of nuclear histone deacetylases by EP300 disrupts the MiCEE complex in idiopathic pulmonary fibrosis. Nat Commun 10:2229 (2019). PubMed: 31110176
- Ye Q et al. Frenolicin B Targets Peroxiredoxin 1 and Glutaredoxin 3 to Trigger ROS/4E-BP1-Mediated Antitumor Effects. Cell Chem Biol 26:366-377.e12 (2019). PubMed: 30661989
- Yamamoto-Fukuda T et al. Keratinocyte Growth Factor (KGF) Modulates Epidermal Progenitor Cell Kinetics through Activation of p63 in Middle Ear Cholesteatoma. J Assoc Res Otolaryngol 19:223-241 (2018). PubMed: 29549594