製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Biotin
  • 特異性
    Reacts with free biotin and biotinylated antibody and proteins
  • アプリケーション
    適用あり: WB, Competitive ELISA, IP, ICC/IFmore details
  • 免疫原

    Full length protein conjugated to KLH

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab53494 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent dilution. Predicted molecular weight: 1 kDa.
Competitive ELISA Use at an assay dependent dilution. (sensitivity: 0.01 ng/mL).
IP Use at an assay dependent dilution.
ICC/IF Use at an assay dependent dilution.

ターゲット情報

  • 関連性
    Biotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da.
  • 別名
    • Vitamin B4 antibody

Anti-Biotin antibody (ab53494) 使用論文

This product has been referenced in:
  • Burgess SG  et al. Allosteric inhibition of Aurora-A kinase by a synthetic vNAR domain. Open Biol 6:N/A (2016). WB . Read more (PubMed: 27411893) »
  • Caron JM & Caron JM Methyl Sulfone Blocked Multiple Hypoxia- and Non-Hypoxia-Induced Metastatic Targets in Breast Cancer Cells and Melanoma Cells. PLoS One 10:e0141565 (2015). Read more (PubMed: 26536104) »

See all 4 Publications for this product

Product Wall

Application
Immunoprecipitation
Immuno-precipitation step
Protein G
Sample
Human Cell lysate - whole cell (RWP1)
Specification
RWP1
Total protein in input
500 µg
Username

Abcam user community

Verified customer

投稿 Feb 14 2014

Thank you for contacting us.

The optimal dilutions or concentrations has to be determined by end user. We can recommend using the antibody at starting dilution 10ug/ml.

I hope this information is helpful to you. Please do not hesitate...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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