製品の概要

  • 製品名Anti-Bim antibody [Y36]
    Bim 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [Y36] to Bim
  • 特異性The antibody can recognize all isoforms of Bim. The antibody does not cross-react with other Bcl-2 family members.
  • アプリケーション適用あり: IHC-FoFr, WB, IHC-P, Flow Cyt, IP, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    A synthetic peptide corresponding to residues in the exons 2 of human Bim.

  • ポジティブ・コントロール
    • WB : Raji, Jurkat, A431, Molt-4, A20, MEF, Raw264.7 and PC-12 cell lysate; Human and mouse thymus, mouse and rat spleen tissue lysate. IHC : breast carcinoma tissue. ICC/IF : A20 and Raji cells. FC : A431 and Raji whole cell lysate. IP : Raji whole cell lysate.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Alternative versions available:

    Anti-Bim antibody (Alexa Fluor® 488) [Y36] (ab200667)

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab32158 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-FoFr 1/100.
WB 1/500 - 1/2000. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).Can be blocked with Bim peptide (ab179844).
IHC-P 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Flow Cyt 1/50 - 1/100.
IP 1/40 - 1/50.
ICC/IF 1/100 - 1/250.

ターゲット情報

  • 機能Induces apoptosis. Isoform BimL is more potent than isoform BimEL. Isoform Bim-alpha1, isoform Bim-alpha2 and isoform Bim-alpha3 induce apoptosis, although less potent than the isoforms BimEL, BimL and BimS. Isoform Bim-gamma induces apoptosis.
  • 組織特異性Isoform BimEL, isoform BimL and isoform BimS are the predominant isoforms and are ubiquitously expressed with a tissue-specific variation. Isoform Bim-gamma is most abundantly expressed in small intestine and colon, and in lower levels in spleen, prostate, testis, heart, liver and kidney.
  • 配列類似性Belongs to the Bcl-2 family.
  • ドメインThe BH3 motif is required for Bcl-2 binding and cytotoxicity.
  • 細胞内局在Mitochondrion and Endomembrane system. Associated with intracytoplasmic membranes.
  • Information by UniProt
  • 参照データベース
  • 別名
    • BCL2 like 11 antibody
    • B2L11_HUMAN antibody
    • BAM antibody
    • Bcl 2 interacting protein Bim antibody
    • Bcl 2 related ovarian death agonist antibody
    • Bcl-2-like protein 11 antibody
    • BCL2 interacting mediator of cell death antibody
    • BCL2 like 11 (apoptosis facilitator) antibody
    • BCL2 like protein 11 antibody
    • Bcl2-interacting mediator of cell death antibody
    • Bcl2-L-11 antibody
    • Bcl2l11 antibody
    • BIM alpha6 antibody
    • BIM antibody
    • BIM beta6 antibody
    • BIM beta7 antibody
    • BimEL antibody
    • BimL antibody
    • BOD antibody
    see all

Anti-Bim antibody [Y36] 画像

  • ab32158 staining Bim in Human breast cancer tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Antigen retrieval was by heat mediated antigen retrieval using Tris/EDTA Buffer, PH9 (ab93684). Samples were incubated with primary antibody (1/100 in blocking buffer) and a Biotin-conjugated Donkey anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody. Cytoplasmic staining can be seen in the human breast cancer cells. Hematoxylin was used as a counter stain.

  • All lanes : Anti-Bim antibody [Y36] (ab32158) at 1/2000 dilution

    Lane 1 : Raji (human Burkitt's lymphoma) whole cell lysate
    Lane 2 : A431 (human epidermoid carcinoma) whole cell lysate
    Lane 3 : Molt-4 (human acute lymphoblastic leukemia) whole cell lysate
    Lane 4 : Human thymus tissue lysate
    Lane 5 : Mouse thymus tissue lysate
    Lane 6 : A20 (mouse reticulum cell sarcoma) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size : 22 kDa

    Observed band size : 22, 18 kDa

    Exposure time : Lane 1- 5: 3 minutes; Lane 6: 2 seconds

    Blocking/Diluting buffer and concentration : 5% NFDM /TBST 

    The observed molecular weight is consistent with the literature (PMID: 24872388)

  • Immunocytochemistry/Immunofluorescence analysis of A20 (Mouse reticulum sarcoma cell line) labeling Bim with ab32158 at a dilution of 1/250. Cells were fixed with 100% methanol. Ab150077 (1/1000) was used as the secondary antibody. Cells were co-stained with ab7291, a mouse anti-tubulin antibody (1/200) using ab150120 as the secondary. Nuclei were counterstained with DAPI (blue). 

    Secondary antibody only control, cells without incubation with the primary antibody was used as negative control.

    Confocal image showing cytoplasmic staining on A20 cell line

  • Immunohistochemical analysis of paraffin-embedded breast carcinoma using ab32158 at 1/100 dilution.
  • All lanes : Anti-Bim antibody [Y36] (ab32158) at 1/2000 dilution

    Lane 1 : Mouse spleen tissue lysate
    Lane 2 : Rat spleen tissue lysate
    Lane 3 : PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate
    Lane 4 : Raw264.7 (mouse abelson murine leukemia virus-induced tumor) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size : 22 kDa

    Observed band size : 22, 18 kDa

    Exposure time : Lane 1-3: 3 minutes; Lane 4: 10 seconds

    Blocking/Diluting buffer and concentration : 5% NFDM /TBST

    The observed molecular weight is consistent with the literature (PMID: 24872388)

  • Immunocytochemistry/Immunofluorescence analysis of Raji (Human Burkitt's lymphoma cell line) labeling Bim with ab32158 at a dilution of 1/250. Cells were fixed with 100% methanol. Ab150077 (1/1000) was used as the secondary antibody. Cells were co-stained with ab7291, a mouse anti-tubulin antibody (1/200) using ab150120 as the secondary. Nuclei were counterstained with DAPI (blue).

    Secondary antibody only control, cells without incubation with the primary antibody was used as negative control.

    Confocal image showing cytoplasmic staining on Raji cell line

  • All lanes : Anti-Bim antibody [Y36] (ab32158) at 1/1000 dilution

    Lane 1 : A20 (mouse reticulum cell sarcoma) whole cell lysate
    Lane 2 : Raji (human Burkitt's lymphoma) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size : 22 kDa

    Observed band size: 22,18,12 kDa

    Exposure time : 1 minute

    Blocking/ Diluting buffer and concentration : 5% NFDM/TBST

    The observed molecular weight is consistent with the literature (PMID: 24872388)

  • Flow Cytometry analysis of Raji (human Burkitt's lymphoma) whole cell lysate labeling Bim with ab32158 at 1/100 (red). Cells were fixed with 4% paraformaldehyde. An Alexa Fluor® 488)-conjugated goat anti-rabbit IgG (1/2000) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG (ab172730). Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.

  • Ab32158 at 1/50 immunoprecipitating Bim in Raji (human Burkitt's lymphoma) whole cell lysate.

    Lane 1 (input): Raji whole cell lysate (10µg)

    Lane 2 (+): ab32158 + Raji whole cell lysate.

    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab32158 in Raji whole cell lysate.

    For western blotting, ab32158 (1/1000) was used as the primary antibody and ab131366 VeriBlot for IP (HRP) was used as the secondary antibody (1/10 000).

     

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Flow Cytometry analysis of A431 (human epidermoid carcinoma) cells labelling Bim with ab32158 at 1/50 (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/2000) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.

  • Anti-Bim antibody [Y36] (ab32158) at 1/500 dilution + Whole cell lysates prepared from human jurkat cells at 200000 cells

    Secondary
    HRP conjugated Donkey polyclonal to rabbit IgG at 1/2000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 22 kDa
    Observed band size : 22 kDa


    Exposure time : 30 seconds

    This image is courtesy of an anonymous abreview.

    Primary diluted in PBS (5% BSA + 0.1% tween20) and incubated with sample for 1 hour and 30 minutes at 20°C.

  • All lanes : Anti-Bim antibody [Y36] (ab32158) at 1/1000 dilution

    Lane 1 : MEF DMSO control whole cell lysate
    Lane 2 : MEF cells treated with 2 µM SAHA for 24 hours whole cell lysate

    Lysates/proteins at 60 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Predicted band size : 22 kDa

    This image is courtesy of an abreview submitted by Christian Marx

    Observed bands : 24, 18 kDa

    Blocking buffer : 5% milk.

    Dilutiing buffer : 1% Milk in PBS-T buffer

    See Abreview

Anti-Bim antibody [Y36] (ab32158) 使用論文

This product has been referenced in:
  • Sheng YH  et al. MUC13 protects colorectal cancer cells from death by activating the NF-?B pathway and is a potential therapeutic target. Oncogene N/A:N/A (2016). Read more (PubMed: 27399336) »
  • Chi HC  et al. Chemotherapy resistance and metastasis-promoting effects of thyroid hormone in hepatocarcinoma cells are mediated by suppression of FoxO1 and Bim pathway. Cell Death Dis 7:e2324 (2016). WB . Read more (PubMed: 27490929) »

See all 13 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Immuno-precipitation step Protein A/G
Sample Human Cell lysate - whole cell (HCT116 p53-/- cell line)
Specification HCT116 p53-/- cell line
Total protein in input 100 µg
Username

Mr. Christian Marx

Verified customer

投稿 Aug 27 2013

Application Western blot
Sample Mouse Cell lysate - whole cell (MEF cell line)
Loading amount 60 µg
Specification MEF cell line
Treatment DMSO control and 2 µM SAHA for 24 hrs
Gel Running Conditions Reduced Denaturing (13%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Mr. Christian Marx

Verified customer

投稿 Feb 13 2013

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - other (HCT116 colon cancer cell line wt and p53 negative)
Loading amount 60 µg
Specification HCT116 colon cancer cell line wt and p53 negative
Gel Running Conditions Reduced Denaturing (10)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Mr. Christian Marx

Verified customer

投稿 Oct 26 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Jurkat cells)
Loading amount 200000 cells
Specification Jurkat cells
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

投稿 Nov 24 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"