Anti-Bim 抗体, prediluted (ab15185)

製品の概要

  • 製品名
    Anti-Bim antibody, prediluted
    Bim 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Bim, prediluted
  • アプリケーション
    適用あり: IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat
  • 免疫原

    Synthetic peptide (Human) (N terminal).

  • ポジティブ・コントロール
    • HeLa cells, Placenta

アプリケーション

Our Abpromise guarantee covers the use of ab15185 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P
  • 追加情報
    IHC-P: 1/1. Perform antigen retrieval before commencing with IHC staining protocol by boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能
      Induces apoptosis. Isoform BimL is more potent than isoform BimEL. Isoform Bim-alpha1, isoform Bim-alpha2 and isoform Bim-alpha3 induce apoptosis, although less potent than the isoforms BimEL, BimL and BimS. Isoform Bim-gamma induces apoptosis.
    • 組織特異性
      Isoform BimEL, isoform BimL and isoform BimS are the predominant isoforms and are ubiquitously expressed with a tissue-specific variation. Isoform Bim-gamma is most abundantly expressed in small intestine and colon, and in lower levels in spleen, prostate, testis, heart, liver and kidney.
    • 配列類似性
      Belongs to the Bcl-2 family.
    • ドメイン
      The BH3 motif is required for Bcl-2 binding and cytotoxicity.
    • 細胞内局在
      Mitochondrion and Endomembrane system. Associated with intracytoplasmic membranes.
    • Information by UniProt
    • 参照データベース
    • 別名
      • BCL2 like 11 antibody
      • B2L11_HUMAN antibody
      • BAM antibody
      • Bcl 2 interacting protein Bim antibody
      • Bcl 2 related ovarian death agonist antibody
      • Bcl-2-like protein 11 antibody
      • BCL2 interacting mediator of cell death antibody
      • BCL2 like 11 (apoptosis facilitator) antibody
      • BCL2 like protein 11 antibody
      • Bcl2-interacting mediator of cell death antibody
      • Bcl2-L-11 antibody
      • Bcl2l11 antibody
      • BIM alpha6 antibody
      • BIM antibody
      • BIM beta6 antibody
      • BIM beta7 antibody
      • BimEL antibody
      • BimL antibody
      • BOD antibody
      see all

    プロトコール

    参考文献

    ab15185 has not yet been referenced specifically in any publications.

    レビューと Q&A

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (pancreas)
    Specification
    pancreas
    Fixative
    Paraformaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 10mm citric
    Permeabilization
    No
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C
    Username

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    Verified customer

    投稿 Sep 12 2007

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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