製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Bim
  • アプリケーション
    適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    Synthetic peptide (Human) (N terminal).

  • ポジティブ・コントロール
    • HeLa cells. Placenta

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab15184 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 17 kDa.
IHC-P 1/25. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.

ターゲット情報

  • 機能
    Induces apoptosis. Isoform BimL is more potent than isoform BimEL. Isoform Bim-alpha1, isoform Bim-alpha2 and isoform Bim-alpha3 induce apoptosis, although less potent than the isoforms BimEL, BimL and BimS. Isoform Bim-gamma induces apoptosis.
  • 組織特異性
    Isoform BimEL, isoform BimL and isoform BimS are the predominant isoforms and are ubiquitously expressed with a tissue-specific variation. Isoform Bim-gamma is most abundantly expressed in small intestine and colon, and in lower levels in spleen, prostate, testis, heart, liver and kidney.
  • 配列類似性
    Belongs to the Bcl-2 family.
  • ドメイン
    The BH3 motif is required for Bcl-2 binding and cytotoxicity.
  • 細胞内局在
    Mitochondrion and Endomembrane system. Associated with intracytoplasmic membranes.
  • Information by UniProt
  • 参照データベース
  • 別名
    • BCL2 like 11 antibody
    • B2L11_HUMAN antibody
    • BAM antibody
    • Bcl 2 interacting protein Bim antibody
    • Bcl 2 related ovarian death agonist antibody
    • Bcl-2-like protein 11 antibody
    • BCL2 interacting mediator of cell death antibody
    • BCL2 like 11 (apoptosis facilitator) antibody
    • BCL2 like protein 11 antibody
    • Bcl2-interacting mediator of cell death antibody
    • Bcl2-L-11 antibody
    • Bcl2l11 antibody
    • BIM alpha6 antibody
    • BIM antibody
    • BIM beta6 antibody
    • BIM beta7 antibody
    • BimEL antibody
    • BimL antibody
    • BOD antibody
    see all

画像

  • ab15184, at 1:25 dilution, staining Bim in human placenta by Immunohistochemistry (Formalin/ PFA fixed paraffin embedded tissue sections).
  • All lanes : Anti-Bim antibody (ab15184)

    Lane 1 : Total cell protein extracts prepared from CEMC7-14 cells, un-treated.
    Lane 2 : Total cell protein extracts prepared from CEMC7-14 cells, treated with dexamethasone (1µM) for 2 hours.
    Lane 3 : Total cell protein extracts prepared from CEMC7-14 cells, treated with dexamethasone (1µM) for 6 hours.
    Lane 4 : Total cell protein extracts prepared from CEMC7-14 cells, treated with dexamethasone (1µM) for 24 hours.

    Developed using the ECL technique

    Predicted band size : 17 kDa
    Observed band size : 22 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 13 kDa (possible isoform),16 kDa (possible isoform).

    Image from Dr JT Lynch et al, Mol Cancer. 2010 Feb 15;9:38, Fig 5.

参考文献

This product has been referenced in:
  • Li Y  et al. IL-7 Receptor Mutations and Steroid Resistance in Pediatric T cell Acute Lymphoblastic Leukemia: A Genome Sequencing Study. PLoS Med 13:e1002200 (2016). WB ; Human . Read more (PubMed: 27997540) »
  • Lynch JT  et al. The role of glucocorticoid receptor phosphorylation in Mcl-1 and NOXA gene expression. Mol Cancer 9:38 (2010). WB ; Human . Read more (PubMed: 20156337) »

See all 3 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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